scholarly journals Analysis of miRNA expression associated with the Lr46 gene responsible for APR resistance in wheat (Triticum aestivum L.)

2020 ◽  
Vol 61 (4) ◽  
pp. 503-511 ◽  
Author(s):  
Agnieszka Tomkowiak ◽  
Tomasz Jędrzejewski ◽  
Julia Spychała ◽  
Jakub Kuczyński ◽  
Michał T. Kwiatek ◽  
...  
Keyword(s):  
Leaf Rust ◽  
Mirna Expression ◽  
Rust Resistance ◽  
Fungal Spores ◽  
Puccinia Triticina ◽  
Leaf Rust Resistance ◽  
Triticum Aestivum L ◽  
Resistance Mechanisms ◽  
Wheat Cultivars ◽  
Slow Rusting

Abstract Lr46/Yr29/Pm39 (Lr46) is a gene for slow rusting resistance in wheat. The aim of the study was to analyze the miRNA expression in selected common wheat cultivars carrying resistance genes, Lr46 among others (HN Rod, Pavon‘S’, Myna‘S’, Frontana‘S’, and Sparrow’S’) in response to leaf rust infection caused by Puccinia triticina Erikss. In the Pavon ‘S’, Myna ‘S’, Frontana‘S’, and Sparow‘S’ varieties a product with a length of 242 bp has been identified, which is specific to the Xwmc44 marker linked to the brown rust resistance gene Lr46. In the next step, the differences in the expression of microRNA (miR5085 and miR164) associated with the Lr46 gene, which is responsible for different resistance of selected wheat cultivars to leaf rust, were examined using emulsion PCR (ddPCR). In the experiment, biotic stress was induced in mature plants by infecting them with fungal spores under controlled conditions in a growth chamber. For analysis the plant material was collected before inoculation and 6, 12, 24, and 48 h after inoculation. The experiments also showed that plant infection with Puccinia triticina resulted in an increase in miR164 expression in cultivars carrying the Lr46 gene. The expression of miR164 remained stable in a control cultivar (HN ROD) lacking this gene. This has proved that miR164 can be involved in leaf rust resistance mechanisms.

scholarly journals Seedling and Slow Rusting Resistance to Leaf Rust in Chinese Wheat Cultivars

Plant Disease ◽  
2010 ◽  
Vol 94 (1) ◽  
pp. 45-53 ◽  
Author(s):  
Z. F. Li ◽  
X. C. Xia ◽  
Z. H. He ◽  
X. Li ◽  
L. J. Zhang ◽  
...  
Keyword(s):  
Leaf Rust ◽  
Resistance Genes ◽  
Rust Resistance ◽  
Puccinia Triticina ◽  
Leaf Rust Resistance ◽  
Wheat Cultivars ◽  
Slow Rusting ◽  
Cropping Seasons ◽  
Rust Resistance Genes ◽  
Leaf Rust Resistance Genes

Identification of resistance genes is important for developing leaf rust resistant wheat (Triticum aestivum) cultivars. A total of 102 Chinese winter wheat cultivars and advanced lines were inoculated with 24 pathotypes of Puccinia triticina for postulation of leaf rust resistance genes effective at the seedling stage. These genotypes were also planted in the field for characterization of slow rusting responses to leaf rust in the 2006–07 and 2007–08 cropping seasons. Fourteen leaf rust resistance genes—Lr1, Lr2a, Lr3bg, Lr3ka, Lr14a, Lr16, Lr17a, Lr18, Lr20, Lr23, Lr24, Lr26, Lr34, and LrZH84—either singly or in combinations, were postulated in 65 genotypes, whereas known resistance genes were not identified in the other 37 accessions. Resistance gene Lr26 was present in 44 accessions. Genes Lr14a and Lr34 were each detected in seven entries. Lr1 and Lr3ka were each found in six cultivars, and five lines possessed Lr16. Lr17a and Lr18 were each identified in four lines. Three cultivars were postulated to possess Lr3bg. Genes Lr20, Lr24, and LrZH84 were each present in two cultivars. Each of the genes Lr2a and Lr23 may exist in one line. Fourteen genotypes showed slow leaf rusting resistance in two cropping seasons.

scholarly journals Genetic analysis and molecular mapping of leaf rust resistance genes in the wheat line 5R618

2014 ◽  
Vol 50 (No. 4) ◽  
pp. 262-267 ◽  
Author(s):  
J. Wang ◽  
L. Shi ◽  
L. Zhu ◽  
X. Li ◽  
D. Liu
Keyword(s):  
Leaf Rust ◽  
Resistance Gene ◽  
Rust Resistance ◽  
Molecular Mapping ◽  
Dominant Gene ◽  
Puccinia Triticina ◽  
Leaf Rust Resistance ◽  
Triticum Aestivum L ◽  
Rust Resistance Gene ◽  
Leaf Rust Resistance Gene

The wheat (Triticum aestivum L.) line 5R618, bred at the China Agricultural University, is resistant in the seedling stage to the majority of the current Chinese pathotypes of wheat leaf rust (Puccinia triticina). To identify and map the leaf rust resistance gene in the 5R618 line, F<sub>2</sub> plants and F<sub>2:3</sub> families from a cross between 5R618 and Zhengzhou5389 (susceptible) were inoculated in the greenhouse with the Chinese P. triticina pathotype THJP. Results from the F<sub>2</sub> and F<sub>2:3</sub> populations indicate that a single dominant gene, temporarily designated&nbsp;Lr5R, conferred resistance. Using the molecular marker method, Lr5R was located on the 3DL chromosome. It was closely linked to the markers Xbarc71 and OPJ-09 with genetic distances of 0.9 cM and 1.0 cM, respectively. At present only one designated gene (Lr24) is located on the 3DL chromosome. The genetic distance between Lr5R&nbsp;and Lr24 confirms that Lr5R is a new leaf rust resistance gene.

INHERITANCE OF LEAF RUST RESISTANCE IN WHEAT CULTIVARS RAFAELA AND EAP 26127 AND CHROMOSOME LOCATION.OF GENE Lr17

1977 ◽  
Vol 19 (2) ◽  
pp. 355-358 ◽  
Author(s):  
P. L. Dyck ◽  
E. R. Kerber
Keyword(s):  
Triticum Aestivum ◽  
Leaf Rust ◽  
Rust Resistance ◽  
Leaf Rust Resistance ◽  
Triticum Aestivum L ◽  
Puccinia Recondita ◽  
Wheat Cultivars ◽  
Seedling Resistance

The inheritance of seedling resistance to leaf rust (Puccinia recondita) was studied in wheat (Triticum aestivum L.) cultivars Rafaela and EAP 26127. Rafaela has genes Lr14b and Lr17 while EAP 26127 has Lr17. Lr17 was located on chromosome 2A, possibly the short arm, and was independent of Lr11.

scholarly journals Identification of Leaf Rust Resistance Genes in Chinese Common Wheat Cultivars

Plant Disease ◽  
2017 ◽  
Vol 101 (10) ◽  
pp. 1729-1737 ◽  
Author(s):  
Takele Weldu Gebrewahid ◽  
Zhan-Jun Yao ◽  
Xiao-Cui Yan ◽  
Pu Gao ◽  
Zai-Feng Li
Keyword(s):  
Leaf Rust ◽  
Common Wheat ◽  
Resistance Genes ◽  
Rust Resistance ◽  
Field Tests ◽  
Puccinia Triticina ◽  
Leaf Rust Resistance ◽  
Adult Plant ◽  
Wheat Cultivars ◽  
Lr Genes

Puccinia triticina Eriks. (Pt), the causal agent of wheat (Triticum aestivum L.) leaf rust, is the most widespread disease of common wheat worldwide. In the present study, 83 wheat cultivars from three provinces of China and 36 tester lines with known leaf rust resistance (Lr) genes were inoculated in the greenhouse with 18 Pt pathotypes to identify seedling effective Lr genes. Field tests were also performed to characterize slow leaf rusting responses at the adult plant growth stage in Baoding and Zhoukou in the 2014–15 and 2015–16 cropping seasons. Twelve Lr genes, viz. Lr1, Lr26, Lr3ka, Lr11, Lr10, Lr2b, Lr13, Lr21, Lr34, Lr37, Lr44, and Lr46 either singly or in combination were identified in 41 cultivars. Known Lr genes were not detected in the remaining 42 cultivars. The most commonly identified resistance genes were Lr26 (20 cultivars), Lr46 (18 cultivars), and Lr1 (eight cultivars). Less frequently detected genes included Lr13, Lr34, and Lr37 (each present in four cultivars), Lr10 (three cultivars), and Lr3ka and Lr44 (each in two cultivars). Evidence for the presence of genes Lr11, Lr2b, and Lr21 (each in one cultivar) was also obtained. Seventeen cultivars were found to have slow rusting resistance in both field growing seasons.

scholarly journals Physiologic Specialization of Puccinia triticina in Canada in 1998

Plant Disease ◽  
2001 ◽  
Vol 85 (2) ◽  
pp. 155-158 ◽  
Author(s):  
J. A. Kolmer
Keyword(s):  
Leaf Rust ◽  
Bread Wheat ◽  
Resistance Genes ◽  
Rust Resistance ◽  
Puccinia Triticina ◽  
Leaf Rust Resistance ◽  
Adult Plant ◽  
Wheat Cultivars ◽  
Rust Resistance Genes ◽  
Leaf Rust Resistance Genes

In 1998, leaf rust of wheat (Triticum aestivum), caused by Puccinia triticina, was widespread throughout the prairies of western Canada. Warm summer temperatures with frequent dew periods favored spread of the disease in wheat fields in Manitoba and Saskatchewan. The Canada Prairie Spring wheat cultivars (AC Vista, AC Foremost, AC Crystal) were susceptible to leaf rust, while the bread wheat cultivars with leaf rust resistance genes Lr16 and Lr13 or Lr34 (AC Majestic, AC Domain, AC Barrie) had high to moderate levels of leaf rust infections. Bread wheat cultivars AC Cora, AC Minto, Pasqua, and McKenzie had trace to low levels of leaf rust infection. Thirty-four virulence phenotypes of P. triticina were identified on 16 Thatcher lines, which are near-isogenic for leaf rust resistance genes. Phenotypes with virulence to Lr16 increased to 25% of isolates in Manitoba and Saskatchewan in 1998. Forty-three isolates were also tested for virulence to plants with the adult plant resistance genes Lr12, Lr13, Lr34, and Lr13,34. Most isolates had virulence to Lr12 and Lr13. All isolates had lower infection type on adult plants with Lr34 compared with Thatcher.

Virulence of Puccinia triticina in Turkey and leaf rust resistance in Turkish wheat cultivars

2012 ◽  
Vol 135 (4) ◽  
pp. 703-716 ◽  
Author(s):  
J. A. Kolmer ◽  
Z. Mert ◽  
K. Akan ◽  
L. Demir ◽  
R. Ünsal ◽  
...  
Keyword(s):  
Leaf Rust ◽  
Rust Resistance ◽  
Puccinia Triticina ◽  
Leaf Rust Resistance ◽  
Wheat Cultivars

scholarly journals Identification of Leaf Rust Resistance Genes in Selected Egyptian Wheat Cultivars by Molecular Markers

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
I. A. Imbaby ◽  
M. A. Mahmoud ◽  
M. E. M. Hassan ◽  
A. R. M. Abd-El-Aziz
Keyword(s):  
Molecular Markers ◽  
Leaf Rust ◽  
Resistance Genes ◽  
Rust Resistance ◽  
Puccinia Triticina ◽  
Leaf Rust Resistance ◽  
Wheat Cultivars ◽  
Rust Disease ◽  
Rust Resistance Genes ◽  
Leaf Rust Resistance Genes

Leaf rust, caused byPuccinia triticinaEriks., is a common and widespread disease of wheat (Triticum aestivumL.) in Egypt. Host resistance is the most economical, effective, and ecologically sustainable method of controlling the disease. Molecular markers help to determine leaf rust resistance genes (Lrgenes). The objective of this study was to identifyLrgenes in fifteen wheat cultivars from Egypt. Ten genes,Lr13,Lr19,Lr24,Lr26,Lr34,Lr35 Lr36,Lr37,Lr39, andLr46, were detected in fifteen wheat cultivars using various molecular markers. The most frequently occurring genes in fifteen Egyptian wheat cultivars wereLr13,Lr24,Lr34, andLr36identified in all the cultivars used, followed byLr26andLr35(93%),Lr39(66%),Lr37(53%), andLr46(26.6%) of the cultivars, and finallyLr19was present in 33.3% of cultivars. It is concluded that there was a good variation inLrgenes carried by wheat cultivars commercially grown in Egypt. Therefore, strategies for deploying resistance genes to prolong effective disease resistance are suggested to control wheat leaf rust disease.

scholarly journals Leaf Rust Resistance of Spring, Facultative, and Winter Wheat Cultivars from China

Plant Disease ◽  
1999 ◽  
Vol 83 (7) ◽  
pp. 644-651 ◽  
Author(s):  
R. P. Singh ◽  
W. Q. Chen ◽  
Z. H. He
Keyword(s):  
Leaf Rust ◽  
Puccinia Triticina ◽  
Leaf Rust Resistance ◽  
Adult Plant ◽  
Plant Responses ◽  
Wheat Cultivars ◽  
Slow Rusting ◽  
Winter Wheat Cultivars ◽  
Infection Types ◽  
Winter Growth Habit

Leaf rust, caused by Puccinia triticina, is an important disease of wheat (Triticum aestivum) in China. Sixty-one spring and 102 facultative or winter growth habit wheat cultivars from China and a set of testers, carrying named Lr genes, were evaluated for resistance at the seedling growth stage with an array of Mexican Puccinia triticina races. Variation in seedling infection types of the cultivars was compared with that of the testers, and genes conferring low infection types were postulated. In total, nine named genes, Lr1 (in 13 cultivars), Lr3 (12), Lr3bg (2), Lr10 (1), Lr13 (4), Lr14a (1), Lr16 (49), Lr23 (9), and Lr26 (81), were identified. Thirty-one cultivars displayed intermediate reactions to one or more races that could not be attributed to any named gene. Twenty-eight spring cultivars were also evaluated at two field sites in Mexico using two common races. About half of them displayed good to moderate adult resistance that may be partly due to the presence of slow rusting gene Lr34 in at least seven cultivars. Diversity in adult plant responses of these wheats indicated the presence of additional slow rusting genes. Presence of 1B.1R translocation in 12 wheat cultivars, supposedly derived from intergeneric crosses involving T. durum, Haynaldia villosa, and Avena fatua, indicated that their pedigrees were incorrect.

scholarly journals Lr46: A Gene Conferring Slow-Rusting Resistance to Leaf Rust in Wheat

1998 ◽  
Vol 88 (9) ◽  
pp. 890-894 ◽  
Author(s):  
R. P. Singh ◽  
A. Mujeeb-Kazi ◽  
J. Huerta-Espino
Keyword(s):  
Leaf Rust ◽  
Rust Resistance ◽  
Leaf Rust Resistance ◽  
Adult Plant ◽  
Monosomic Analysis ◽  
Additive Effects ◽  
Wheat Cultivars ◽  
Plant Leaf ◽  
Slow Rusting ◽  
Different Seasons

Wheat (Triticum aestivum) cultivar Pavon 76 carries slow-rusting resistance to leaf rust that has remained effective in Mexico since its release in 1976. ‘Pavon 76’ was crossed with two leaf rust-susceptible wheat cultivars, Jupateco 73S and Avocet S, and between 118 and 148 individual F2 plant-derived F3 and F5 lines were evaluated for adult-plant leaf rust resistance at two field sites in Mexico during different seasons. Evaluation of F1 plants and parents indicated that the slow-rusting resistance was partially dominant. Segregation in the F3 and F5 indicated that the resistance was based on two genes with additive effects. Monosomic analysis was carried out to determine the chromosomal locations of the resistance genes. For this purpose, two or three backcross-derived cytogenetic populations were developed by crossing ‘Pavon 76’ with a monosomic series of adult-plant leaf rust-susceptible cultivar Lal-bahadur. Evaluation of such BC2F3 and BC3F3 lines from 16 confirmed ‘Lalbahadur’ monosomics indicated that one slow-rusting gene was located in chromosome 1B of ‘Pavon 76’. This gene, designated as Lr46, is the second named gene involved in slow-rusting resistance to leaf rust in wheat.

scholarly journals Non-hypersensitive leaf rust resistance of bread wheat cultivar PBW65 conditioned by genes different fromLr34

2009 ◽  
Vol 45 (No. 1) ◽  
pp. 26-30 ◽  
Author(s):  
M.A. Khan ◽  
R.G. Saini
Keyword(s):  
Leaf Rust ◽  
Bread Wheat ◽  
Rust Resistance ◽  
Wheat Cultivar ◽  
Infection Type ◽  
Puccinia Triticina ◽  
Leaf Rust Resistance ◽  
Triticum Aestivum L ◽  
Resistance Level ◽  
Highly Virulent

: The bread wheat (Triticum aestivum L.) cultivar PBW65 has shown hight levels of resistance to the most frequent and highly virulent Indian race 77-5 of leaf rust (Puccinia triticina). The infection type and disease severity indicated a non-hypersensitive type of resistance against the race 77-5 in PBW65. The cultivar PBW65 was crossed with the leaf rust susceptible cultivar WL711 to determine the mode of inheritance of the resistance. The segregation for resistant and susceptible plants in the F<sub>2</sub> and F<sub>3</sub> generations revealed, that two genes, each showing additive effects, were likely to confer resistance to leaf rust in PBW65. Intercrossing of PBW65 with Cook (Lr34), RL6058 (Lr34) and HD2009, possessing a similar resistance level like PBW65, revealed that the genes for leaf rust resistance in PBW65 were non-allelic to Cook (Lr34), RL6058 (Lr34) as well as to the gene(s) in HD2009. It is concluded that the cultivar PBW65 is a novel source of non-hypersensitive leaf rust resistance.

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