Crude glycerine characterization: analysis of free fatty acids, fatty acid methyl esters, and acylglycerides

2020 ◽  
Author(s):  
Maira Alejandra Maquirriain ◽  
Lucas Gabriel Tonutti ◽  
Carlos Alberto Querini ◽  
María Laura Pisarello
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Free Fatty Acids ◽  
Fatty Acid Methyl Esters ◽  
Methyl Esters ◽  
Crude Glycerine ◽  
Acid Methyl

scholarly journals Synthesis of Fatty Acid Methyl Esters from Jatropha curcas Oil and Its Purification Using Solvent Fractionation

2014 ◽  
Vol 13 (1) ◽  
pp. 31
Author(s):  
Avita Kusuma Wardhani ◽  
Chusnul Hidayat
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Free Fatty Acids ◽  
Jatropha Curcas ◽  
Fatty Acid Methyl Esters ◽  
Methyl Esters ◽  
Solvent Fractionation ◽  
Jatropha Curcas Oil ◽  
Acid Methyl ◽  
Purification Factor

Fatty acid methyl esters (FAME) are produced by transesterification. The problem in the product of transesterification is the presence of impurities such as mono-, di-, triglycerides, and free fatty acids. So that, the purification using solvent fractionation is needed to separate them from FAME. The objective of this research were to determine the effects of crude fatty acid methyl esters-to-acetone (CFAME/acetone) ratio on yield, purity, purification factor, and recovery of FAME after fractionation and to evaluate the impurities which were separated in each step of fractionation. FAME were produced from Jatropha curcas oil using Berchmans’s and Tiwari’s methods. The impurities were separated by solvent fractionation using acetone. CFAME/acetone ratios were 1, 2, 3, 4, and 5. Fractionation was done stepwise namely 21°C, 16°C, 12°C, and 5°C. The results showed that the conversion of FAME using Tiwari’s method was 1.7-fold higher than Berchmans’s method. Purification of FAME using solvent fractionation resulted that the best CFAME/acetone ratio was 1. Yield decreased 1.6-fold at CFAME/acetone ratio 4. Purity decreased 8.74% with an increase in CFAME/acetone ratio 1 to 5. Purification factor decreased 2-fold at CFAME/acetone 1 to 3. Recovery decreased 1.3-fold at CFAME/acetone ratio 1 to 4. The impurities which were separated from FAME were mono-, di-, triglycerides, and free fatty acids and the major component of impurities was triglycerides (>59%). The results indicated that solvent fractionation could be used as an alternative method for purifying FAME and further study to optimize this method was needed.

scholarly journals Composition Characterization of Fatty Acid Zinc Salts by Chromatographic and NMR Spectroscopic Analyses on Their Fatty Acid Methyl Esters

2019 ◽  
Vol 2019 ◽  
pp. 1-11
Author(s):  
Kwang Seo Park ◽  
Yun Ju Kim ◽  
Eun Kyung Choe
Keyword(s):  
Mass Spectrometry ◽  
Fatty Acids ◽  
Gas Chromatography ◽  
Fatty Acid ◽  
Free Fatty Acids ◽  
Fatty Acid Methyl Esters ◽  
Methyl Esters ◽  
Gas Chromatography Mass Spectrometry ◽  
Acid Methyl ◽  
Zinc Salts

To implement EU REACH- (Registration, Evaluation, and Authorization of Chemicals-) like chemical legislations in various countries of which the purpose is human and environment safety, the first step is substance identification followed by the hazard and risk assessments. Although both structural and composition identifications are required, the latter can more importantly result in the essential data to fill out the required substance information such as purity and concentrations of constituents, as well as impurities. With fatty acid zinc salts (FAZSs) as an exemplary industrial chemical of which chromatographic and nuclear magnetic resonance (NMR) analyses were impossible due to their insolubility in water and any organic solvents, the composition characterization was tried by preparing their fatty acid methyl esters (FAMEs) using the conc. HCl/methanol/toluene method. This acid-catalyzed methyl esterification was optimized with zinc stearate as a surrogate substance. Gas chromatography-mass spectrometry (GC-MS) and NMR analyses on methyl-esterified products revealed that the optimum conditions were at 90°C for 10 min or 45°C for 30 min with two equivalent HCl as well as at 45°C for 10 min with five equivalent HCl. Almost all zinc stearates were converted into the corresponding fatty acids with 97–99% conversion rates. Free fatty acids (FFAs) were detected in extracted ion chromatograms of pyrolysis-gas chromatography-mass spectrometry (Py-GC-MS) in the methyl-esterified products with incomplete conversions of 73∼79%. The optimized conc. HCl/methanol/toluene method of direct one-step reaction from FAZSs was compared with the two-step NaOH saponification/BF3-methanol method after acidic hydrolysis of FAZSs. The mechanism of fatty acid zinc salts into free fatty acids and fatty acid methyl esters was suggested with the evidence of the formation of Zn(OH)2.

scholarly journals Engineering Escherichia coli for Biodiesel Production Utilizing a Bacterial Fatty Acid Methyltransferase

2011 ◽  
Vol 77 (22) ◽  
pp. 8052-8061 ◽  
Author(s):  
Parwez Nawabi ◽  
Stefan Bauer ◽  
Nikos Kyrpides ◽  
Athanasios Lykidis
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Free Fatty Acids ◽  
Fatty Acid Methyl Esters ◽  
Methyl Esters ◽  
Acyl Chain ◽  
Biodiesel Production ◽  
Common Component ◽  
Acid Methyl ◽  
Bacterial Fatty Acid

ABSTRACTThe production of low-cost biofuels in engineered microorganisms is of great interest due to the continual increase in the world's energy demands. Biodiesel is a renewable fuel that can potentially be produced in microbes cost-effectively. Fatty acid methyl esters (FAMEs) are a common component of biodiesel and can be synthesized from either triacylglycerol or free fatty acids (FFAs). Here we report the identification of a novel bacterial fatty acid methyltransferase (FAMT) that catalyzes the formation of FAMEs and 3-hydroxyl fatty acid methyl esters (3-OH-FAMEs) from the respective free acids andS-adenosylmethionine (AdoMet). FAMT exhibits a higher specificity toward 3-hydroxy free fatty acids (3-OH-FFAs) than FFAs, synthesizing 3-hydroxy fatty acid methyl esters (3-OH-FAMEs)in vivo. We have also identified bacterial members of the fatty acyl-acyl carrier protein (ACP) thioesterase (FAT) enzyme family with distinct acyl chain specificities. These bacterial FATs exhibit increased specificity toward 3-hydroxyacyl-ACP, generating 3-OH-FFAs, which can subsequently be utilized by FAMTs to produce 3-OH-FAMEs. PhaG (3-hydroxyacyl ACP:coenzyme A [CoA] transacylase) constitutes an alternative route to 3-OH-FFA synthesis; the coexpression of PhaG with FAMT led to the highest level of accumulation of 3-OH-FAMEs and FAMEs. The availability of AdoMet, the second substrate for FAMT, is an important factor regulating the amount of methyl esters produced by bacterial cells. Our results indicate that the deletion of the global methionine regulatormetJand the overexpression of methionine adenosyltransferase result in increased methyl ester synthesis.

Producing Fatty Acid Methyl Esters from Free Fatty Acids with Ionic Liquids as Catalyst

2018 ◽  
Vol 42 (1) ◽  
pp. 174-181 ◽  
Author(s):  
Fangxia Yang ◽  
Junhua Zhang ◽  
Yingjie Guo ◽  
Lele Feng ◽  
Shijun Wei ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Ionic Liquids ◽  
Fatty Acid ◽  
Free Fatty Acids ◽  
Fatty Acid Methyl Esters ◽  
Methyl Esters ◽  
Acid Methyl
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