scholarly journals Enhanced production of withaferin-A in shoot cultures of Withania somnifera (L) Dunal

2014 ◽  
Vol 23 (4) ◽  
pp. 430-434 ◽  
Author(s):  
Bilal Ahmad Mir ◽  
Jabeena Khazir ◽  
Khalid R. Hakeem ◽  
Sushma Koul ◽  
Don A. Cowan
Keyword(s):  
Withania Somnifera ◽  
Withaferin A ◽  
Shoot Cultures ◽  
Enhanced Production

Traditional Horticulture Practices Increase the Production of Selected Withanolides in Withania Somnifera (L.) Dunal—A RP-UFLC Analysis

2020 ◽  
Vol 58 (10) ◽  
pp. 899-906
Author(s):  
Gireesh M Ankad ◽  
Sandeep R Pai ◽  
Jagadishchandra Hiremath ◽  
Harsha V Hegde
Keyword(s):  
Withania Somnifera ◽  
Farmyard Manure ◽  
Simultaneous Detection ◽  
Withaferin A ◽  
Reverse Phase ◽  
Ancient India ◽  
Enhanced Production ◽  
Plant Life ◽  
Multiple Comparison Test ◽  
Withanolide A

Abstract The study evaluates the effect of two traditional horticulture treatments mentioned in Vrikshayurveda, a text from ancient India on the science of plant life, namely Kunapa jala (KJ) and Pancha gavya (PG) on the production of Withaferin A (WFA), withanolide A (WIA) and Withanolide B (WIB) in Withania somnifera (L) Dunal. Leaves and roots of W. somnifera were collected from different treated groups viz. control, KJ, PG, farmyard manure (FYM) and inorganic fertilizer (NPK). Reverse phase ultra-flow liquid chromatography (RP-UFLC) method was developed, validated for simultaneous detection of WFA, WIA and WIB. Statistical analysis of data was performed by ANOVA and tested for significance by the Dunnett multiple comparison test and data were expressed as mean ± standard deviation (SD). Results revealed, leaves possessed highest WFA content and roots possessed highest content of WIA and WIB. PG treated leaves were observed highest WFA (18.29 mg/g) and roots were observed highest WIA (19.63 mg/g) and WIB (1.36 mg/g). Conclusively, RP-UFLC method for simultaneous detection of withanolides has been developed and validated to evaluate the effect of traditional horticulture treatments. It is concluded that the enhanced production of withanolides can be achieved by the application of PG when compared to NPK application.

Production of Withaferin A in Shoot Cultures of Withania somnifera

Planta Medica ◽  
2001 ◽  
Vol 67 (5) ◽  
pp. 432-436 ◽  
Author(s):  
Swagata Ray ◽  
Sumita Jha*
Keyword(s):  
Withania Somnifera ◽  
Withaferin A ◽  
Shoot Cultures

scholarly journals Pharmacological Levels of Withaferin A (Withania somnifera) Trigger Clinically Relevant Anticancer Effects Specific to Triple Negative Breast Cancer Cells

PLoS ONE ◽  
2014 ◽  
Vol 9 (2) ◽  
pp. e87850 ◽  
Author(s):  
Katarzyna Szarc vel Szic ◽  
Ken Op de Beeck ◽  
Dariusz Ratman ◽  
An Wouters ◽  
Ilse M. Beck ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Triple Negative Breast Cancer ◽  
Breast Cancer Cells ◽  
Withania Somnifera ◽  
Triple Negative ◽  
Withaferin A ◽  
Anticancer Effects

scholarly journals Neuroprotective Effect of CR-777, a Glutathione Derivative of Withaferin A, Obtained through the Bioconversion of Withania somnifera (L.) Dunal Extract by the Fungus Beauveria bassiana

Molecules ◽  
2019 ◽  
Vol 24 (24) ◽  
pp. 4599 ◽  
Author(s):  
Chérif Rabhi ◽  
Guillaume Arcile ◽  
Géraldine Le Goff ◽  
Christian Da Costa Noble ◽  
Jamal Ouazzani
Keyword(s):  
Beauveria Bassiana ◽  
Cortical Neurons ◽  
Withania Somnifera ◽  
Neuroprotective Effect ◽  
2D Nmr ◽  
Withaferin A ◽  
Protective Agent ◽  
Specific Staining ◽  
6 Hydroxydopamine ◽  
Fungus Beauveria Bassiana

The bioconversion of Withania somnifera extract by the fungus Beauveria bassiana leads to cysteine and glutathione derivatives of withaferin A at the C-6 position. The compounds were purified and fully characterized by 1D-NMR, 2D-NMR, and HRMS analysis. The glutathione derivative CR-777 was evaluated as a neuroprotective agent from damage caused by different neurotoxins mimicking molecular symptoms in Parkinson´s disease (PD), including 1-methyl-4-phenylpyridinium (MPP+), 6-hydroxydopamine (6-OHDA), and α-synuclein (α-Syn). CR-777, at nanomolar concentrations, protected dopaminergic and cortical neurons. In 6-OHDA-treated neurons, CR-777 increased cell survival and neurite network and decreased the expression of α-Syn. Using specific inhibitors of cell toxicity signaling pathways and specific staining experiments, the observed role of CR-777 seemed to involve the PI3K/mTOR pathway. CR-777 could be considered as a protective agent against a large panel of neuronal stressors and was engaged in further therapeutic development steps.

scholarly journals Production of the Anticancer Compound Withaferin A from Genetically Transformed Hairy Root Cultures of Withania Somnifera

2018 ◽  
Vol 13 (8) ◽  
pp. 1934578X1801300
Author(s):  
Zeynab Yousefian ◽  
Behnaz Hosseini ◽  
Hassan Rezadoost ◽  
Javier Palazón ◽  
Mohammad Hossein Mirjalili
Keyword(s):  
Hairy Roots ◽  
Withania Somnifera ◽  
Growth Index ◽  
Dry Weight ◽  
Squalene Synthase ◽  
Withaferin A ◽  
Farnesyl Pyrophosphate ◽  
Transformed Hairy Roots ◽  
Transformed Hairy Root ◽  
Anticancer Compound

Withanolides (WTDs), well-known medicinally important compounds of Withania somnifera, including the anticancer compound withaferin A (WFA), are biosynthesized from their dedicated precursor squalene. Condensation of farnesyl pyrophosphate (FPP) molecules to produce squalene is catalyzed by squalene synthase (SQS). In the present study, the Arabidopsis thaliana squalene synthase gene ( AtSQS1) was transformed in W. somnifera by Agrobacterium tumefaciens C58C1 (pRiA4). The AtSQS1 gene was detected in 87.88 % of induced transformed hairy roots (THRs). The results showed that the growth index (GI) of THRs after five weeks of culture was 2-fold higher than that of adventitious hairy roots (AHRs). The biomass doubling time of THRs and AHRs was 18 and 30 days, respectively. Quantitative evaluation of WFA in the studied roots showed that THRs contain 1.51-fold more WFA (330±0.87μg g−1 dry weight (DW)) than AHRs (218±0.17μg g−1 DW). These findings can be used for the production of WFA as a valuable anticancer compound through controlled and scaled up cultures of W. somnifera THRs.

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