Characterization of proliferation, differentiation potential, and gene expression among clonal cultures of human dental pulp cells

In this study, hydrogels based on chitosan cross-linked by glyoxal have been investigated for potential medical applications. Hydrogels were loaded with tannic acid at different concentrations. The thermal stability and the polyphenol-releasing rate were determined. For a preliminary assessment of the clinical usefulness of the hydrogels, they were examined for blood compatibility and in the culture of human dental pulp cells (hDPC). The results showed that after immersion in a polyphenol solution, chitosan/glyoxal hydrogels remain nonhemolytic for erythrocytes, and we also did not observe the cytotoxic effect of hydrogels immersed in tannic acid (TA) solutions with different concentration. Tannic acid was successfully released from hydrogels, and its addition improved material thermal stability. Thus, the current findings open the possibility to consider such hydrogels in clinics.

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In vitro characterization of human dental pulp cells: various isolation methods and culturing environments

Cell and Tissue Research ◽

10.1007/s00441-005-0117-9 ◽

2006 ◽

Vol 324 (2) ◽

pp. 225-236 ◽

Cited By ~ 118

Author(s):

George T.-J. Huang ◽

Wataru Sonoyama ◽

James Chen ◽

Sang Hyuk Park

Keyword(s):

Dental Pulp ◽

Dental Pulp Cells ◽

Human Dental Pulp Cells ◽

In Vitro Characterization ◽

Isolation Methods ◽

Human Dental Pulp ◽

Pulp Cells

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Sclerostin promotes human dental pulp cells senescence

PeerJ ◽

10.7717/peerj.5808 ◽

2018 ◽

Vol 6 ◽

pp. e5808 ◽

Cited By ~ 4

Author(s):

Yanjing Ou ◽

Yi Zhou ◽

Shanshan Liang ◽

Yining Wang

Keyword(s):

Dental Pulp ◽

Wnt Signaling Pathway ◽

Dental Pulp Cells ◽

Differentiation Potential ◽

Human Dental Pulp Cells ◽

Qrt Pcr ◽

Odontoblastic Differentiation ◽

Human Dental Pulp ◽

Pulp Cells

Background Senescence-related impairment of proliferation and differentiation limits the use of dental pulp cells for tissue regeneration. Deletion of sclerostin improves the dentinogenesis regeneration, while its role in dental pulp senescence is unclear. We investigated the role of sclerostin in subculture-induced senescence of human dental pulp cells (HDPCs) and in the senescence-related decline of proliferation and odontoblastic differentiation. Methods Immunohistochemical staining and qRT-PCR analyses were performed to examine the expression pattern of sclerostin in young (20–30-year-old) and senescent (45–80-year-old) dental pulps. HDPCs were serially subcultured until senescence, and the expression of sclerostin was examined by qRT-PCR analysis. HDPCs with sclerostin overexpression and knockdown were constructed to investigate the role of sclerostin in HDPCs senescence and senescence-related impairment of odontoblastic differentiation potential. Results By immunohistochemistry and qRT-PCR, we found a significantly increased expression level of sclerostin in senescent human dental pulp compared with that of young human dental pulp. Additionally, elevated sclerostin expression was found in subculture-induced senescent HDPCs in vitro. By sclerostin overexpression and knockdown, we found that sclerostin promoted HDPCs senescence-related decline of proliferation and odontoblastic differentiation potential with increased expression of p16, p53 and p21 and downregulation of the Wnt signaling pathway. Discussion The increased expression of sclerostin is responsible for the decline of proliferation and odontoblastic differentiation potential of HDPCs during cellular senescence. Anti-sclerostin treatment may be beneficial for the maintenance of the proliferation and odontoblastic differentiation potentials of HDPCs.

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Highly Proliferative Immortalized Human Dental Pulp Cells Retain the Odontogenic Phenotype when Combined with a Beta-Tricalcium Phosphate Scaffold and BMP2

Stem Cells International ◽

10.1155/2020/4534128 ◽

2020 ◽

Vol 2020 ◽

pp. 1-18 ◽

Cited By ~ 3

Author(s):

Xiangfen Li ◽

Liu Wang ◽

Qin Su ◽

Ling Ye ◽

Xuedong Zhou ◽

...

Keyword(s):

Tricalcium Phosphate ◽

Dental Pulp ◽

Biological Behavior ◽

Dental Pulp Cells ◽

Differentiation Potential ◽

Human Dental Pulp Cells ◽

Beta Tricalcium Phosphate ◽

Human Dental Pulp ◽

Pulp Cells

Human dental pulp cells (HDPCs) play a vital role in dentin formation and reparative dentinogenesis, which indicated their potential application in regenerative medicine. However, HDPCs, which can only be obtained from scarce human pulp tissues, also have a limited lifespan in vitro, and stem cells usually lose their original characteristics over a large number of passages. To overcome these challenges, we successfully immortalized human dental pulp cells using the piggyBac system which was employed to efficiently overexpress the SV40 T-Ag, and we then comprehensively described the cell biological behavior. The immortalized human dental pulp cells (iHDPCs) acquired long-term proliferative activity and expressed most HDPC markers. The iHDPCs maintained multiple differentiation potential and could be induced to differentiate into chondrogenic, osteogenic, and adipogenic cells in vitro. We also proved that the iHDPCs gained a stronger ability to migrate than the primary cells, while apoptosis was inhibited. Furthermore, highly proliferative iHDPCs displayed no oncogenicity when subcutaneously implanted into athymic nude mice. Finally, iHDPCs exhibited odontogenic differentiation ability and secreted dentin sialophosphoprotein (DSPP) when combined with a beta-tricalcium phosphate scaffold and bone morphogenetic protein-2 (BMP2) in vivo. Conclusively, the established iHDPCs are a valuable resource for mechanistic study of dental pulp cell differentiation and dental pulp injury repair, as well as for applications in tooth regeneration.

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