Assessment of wound healing activity of ethanolic extracts of Pistacia lentiscus L. leaves and Quercus ilex L. bark in full thickness skin excision in rats

2021 ◽  
Author(s):  
Houari Hemida ◽  
Koula Doukani ◽  
Abdelkader Zitouni ◽  
Brahim Miloud ◽  
Houcine Beggar ◽  
...  
Keyword(s):  
Wound Healing ◽  
Quercus Ilex ◽  
Pistacia Lentiscus ◽  
Full Thickness ◽  
Full Thickness Skin ◽  
Thickness Skin ◽  
Ethanolic Extracts ◽  
Skin Excision ◽  
Wound Healing Activity

scholarly journals Heparinized silk fibroin hydrogels loading FGF1 promote the wound healing in rats with full-thickness skin excision

2019 ◽  
Vol 18 (1) ◽  
Author(s):  
Sirong He ◽  
Dan Shi ◽  
Zhigang Han ◽  
Zhaoming Dong ◽  
Yajun Xie ◽  
...  
Keyword(s):  
Wound Healing ◽  
Silk Fibroin ◽  
Wound Repair ◽  
Histological Evaluation ◽  
Full Thickness ◽  
Acidic Fibroblast Growth Factor ◽  
Full Thickness Skin ◽  
Thickness Skin ◽  
Skin Excision

Abstract Background Silk fibroin hydrogel, derived from Bombyx mori cocoons, has been shown to have potential effects on wound healing due to its excellent biocompatibility and less immunogenic and biodegradable properties. Many studies suggest silk fibroin as a promising material of wound dressing and it can support the adhesion and proliferation of a variety of human cells in vitro. However, lack of translational evidence has hampered its clinical applications for skin repair. Herein, a heparin-immobilized fibroin hydrogel was fabricated to deliver FGF1 (human acidic fibroblast growth factor 1) on top of wound in rats with full-thickness skin excision by performing comprehensive preclinical studies to fully evaluate its safety and effectiveness. The wound-healing efficiency of developed fibroin hydrogels was evaluated in full-thickness wound model of rats, compared with the chitosan used clinically. Results The water absorption, swelling ratio, accumulative FGF1 releasing rate and biodegradation ratio of fabricated hydrogels were measured. The regenerated fibroin hydrogels with good water uptake properties rapidly swelled to a 17.3-fold maximum swelling behavior over 12 h and a total amount of 40.48 ± 1.28% hydrogels was lost within 15 days. Furthermore, accumulative releasing data suggested that heparinized hydrogels possessed effective release behavior of FGF1. Then full-thickness skin excision was created in rats and left untreated or covered with heparinized fibroin hydrogels-immobilized recombinant human FGF1. The histological evaluation using hematoxylin and eosin (HE) and Masson’s trichrome (MT) staining was performed to observe the dermic formation and collagen deposition on the wound-healing site. To evaluate the wound-healing mechanisms induced by fibroin hydrogel treatment, wound-healing scratch and cell proliferation assay were performed. it was found that both fibroin hydrogels and FGF1 can facilitate the migration of fibroblast L929 cells proliferation and migration. Conclusion This study provides systematic preclinical evidence that the silk fibroin promotes wound healing as a wound-healing dressing, thereby establishing a foundation toward its further application for new treatment options of wound repair and regeneration.

scholarly journals Development of a Novel Pre-Vascularized Three-Dimensional Skin Substitute Using Blood Plasma Gel

2018 ◽  
Vol 27 (10) ◽  
pp. 1535-1547 ◽  
Author(s):  
Niann-Tzyy Dai ◽  
Wen-Shyan Huang ◽  
Fang-Wei Chang ◽  
Lin-Gwei Wei ◽  
Tai-Chun Huang ◽  
...  
Keyword(s):  
Wound Healing ◽  
Human Plasma ◽  
Three Dimensional ◽  
Skin Substitute ◽  
Strength Analysis ◽  
Full Thickness ◽  
Skin Substitutes ◽  
Full Thickness Skin ◽  
Post Surgery ◽  
Thickness Skin

Skin substitutes with existing vascularization are in great demand for the repair of full-thickness skin defects. In the present study, we hypothesized that a pre-vascularized skin substitute can potentially promote wound healing. Novel three-dimensional (3D) skin substitutes were prepared by seeding a mixture of human endothelial progenitor cells (EPCs) and fibroblasts into a human plasma/calcium chloride formed gel scaffold, and seeding keratinocytes onto the surface of the plasma gel. The capacity of the EPCs to differentiate into a vascular-like tubular structure was evaluated using immunohistochemistry analysis and WST-8 assay. Experimental studies in mouse full-thickness skin wound models showed that the pre-vascularized gel scaffold significantly accelerated wound healing 7 days after surgery, and resembled normal skin structures after 14 days post-surgery. Histological analysis revealed that pre-vascularized gel scaffolds were well integrated in the host skin, resulting in the vascularization of both the epidermis and dermis in the wound area. Moreover, mechanical strength analysis demonstrated that the healed wound following the implantation of the pre-vascularized gel scaffolds exhibited good tensile strength. Taken together, this novel pre-vascularized human plasma gel scaffold has great potential in skin tissue engineering.

scholarly journals Pharmaceutical application of frog skin on full-thickness skin wound healing in mice

2013 ◽  
Vol 51 (12) ◽  
pp. 1600-1606 ◽  
Author(s):  
Mahere Rezazade Bazaz ◽  
Mohammad Mashreghi ◽  
Nasser Mahdavi Shahri ◽  
Mansour Mashreghi ◽  
Ahmad Asoodeh ◽  
...  
Keyword(s):  
Wound Healing ◽  
Frog Skin ◽  
Skin Wound ◽  
Full Thickness ◽  
Skin Wound Healing ◽  
Full Thickness Skin ◽  
Pharmaceutical Application ◽  
Thickness Skin

Effects of Nanofiber/Stem Cell Composite on Wound Healing in Acute Full-Thickness Skin Wounds

2011 ◽  
Vol 17 (9-10) ◽  
pp. 1413-1424 ◽  
Author(s):  
Kun Ma ◽  
Susan Liao ◽  
Liumin He ◽  
Jia Lu ◽  
Seeram Ramakrishna ◽  
...  
Keyword(s):  
Wound Healing ◽  
Stem Cell ◽  
Skin Wounds ◽  
Full Thickness ◽  
Full Thickness Skin ◽  
Thickness Skin

Comparison of five dermal substitutes in full-thickness skin wound healing in a porcine model

Burns ◽  
2012 ◽  
Vol 38 (6) ◽  
pp. 820-829 ◽  
Author(s):  
Cécile Philandrianos ◽  
Lucile Andrac-Meyer ◽  
Serge Mordon ◽  
Jean-Marc Feuerstein ◽  
Florence Sabatier ◽  
...  
Keyword(s):  
Wound Healing ◽  
Porcine Model ◽  
Skin Wound ◽  
Full Thickness ◽  
Skin Wound Healing ◽  
Dermal Substitutes ◽  
Full Thickness Skin ◽  
Thickness Skin

Verapamil, a Calcium-Channel Blocker, Improves the Wound Healing Process in Rats with Excisional Full-Thickness Skin Wounds Based on Stereological Parameters

2016 ◽  
Vol 29 (8) ◽  
pp. 371-374 ◽  
Author(s):  
Soheil Ashkani-Esfahani ◽  
Omid Koohi Hosseinabadi ◽  
Parinaz Moezzi ◽  
Yalda Moafpourian ◽  
Sina Kardeh ◽  
...  
Keyword(s):  
Wound Healing ◽  
Calcium Channel ◽  
Calcium Channel Blocker ◽  
Channel Blocker ◽  
Healing Process ◽  
Wound Healing Process ◽  
Skin Wounds ◽  
Full Thickness ◽  
Full Thickness Skin ◽  
Thickness Skin
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