scholarly journals Heparinized silk fibroin hydrogels loading FGF1 promote the wound healing in rats with full-thickness skin excision

2019 ◽  
Vol 18 (1) ◽  
Author(s):  
Sirong He ◽  
Dan Shi ◽  
Zhigang Han ◽  
Zhaoming Dong ◽  
Yajun Xie ◽  
...  
Keyword(s):  
Wound Healing ◽  
Silk Fibroin ◽  
Wound Repair ◽  
Histological Evaluation ◽  
Full Thickness ◽  
Acidic Fibroblast Growth Factor ◽  
Full Thickness Skin ◽  
Thickness Skin ◽  
Skin Excision

Abstract Background Silk fibroin hydrogel, derived from Bombyx mori cocoons, has been shown to have potential effects on wound healing due to its excellent biocompatibility and less immunogenic and biodegradable properties. Many studies suggest silk fibroin as a promising material of wound dressing and it can support the adhesion and proliferation of a variety of human cells in vitro. However, lack of translational evidence has hampered its clinical applications for skin repair. Herein, a heparin-immobilized fibroin hydrogel was fabricated to deliver FGF1 (human acidic fibroblast growth factor 1) on top of wound in rats with full-thickness skin excision by performing comprehensive preclinical studies to fully evaluate its safety and effectiveness. The wound-healing efficiency of developed fibroin hydrogels was evaluated in full-thickness wound model of rats, compared with the chitosan used clinically. Results The water absorption, swelling ratio, accumulative FGF1 releasing rate and biodegradation ratio of fabricated hydrogels were measured. The regenerated fibroin hydrogels with good water uptake properties rapidly swelled to a 17.3-fold maximum swelling behavior over 12 h and a total amount of 40.48 ± 1.28% hydrogels was lost within 15 days. Furthermore, accumulative releasing data suggested that heparinized hydrogels possessed effective release behavior of FGF1. Then full-thickness skin excision was created in rats and left untreated or covered with heparinized fibroin hydrogels-immobilized recombinant human FGF1. The histological evaluation using hematoxylin and eosin (HE) and Masson’s trichrome (MT) staining was performed to observe the dermic formation and collagen deposition on the wound-healing site. To evaluate the wound-healing mechanisms induced by fibroin hydrogel treatment, wound-healing scratch and cell proliferation assay were performed. it was found that both fibroin hydrogels and FGF1 can facilitate the migration of fibroblast L929 cells proliferation and migration. Conclusion This study provides systematic preclinical evidence that the silk fibroin promotes wound healing as a wound-healing dressing, thereby establishing a foundation toward its further application for new treatment options of wound repair and regeneration.

Wound healing effect of silk fibroin/alginate-blended sponge in full thickness skin defect of rat

2006 ◽  
Vol 17 (6) ◽  
pp. 547-552 ◽  
Author(s):  
Dae-Hyun Roh ◽  
Seuk-Yun Kang ◽  
Jeom-Yong Kim ◽  
Young-Bae Kwon ◽  
Hae Young Kweon ◽  
...  
Keyword(s):  
Wound Healing ◽  
Silk Fibroin ◽  
Skin Defect ◽  
Full Thickness ◽  
Full Thickness Skin ◽  
Healing Effect ◽  
Thickness Skin ◽  
Wound Healing Effect

scholarly journals Injectable Self-Healing Ceria-based Nanocomposite Hydrogel with ROS-scavenging Activity for Skin Wound Repair

2021 ◽  
Author(s):  
Xueyun Gong ◽  
Meng Luo ◽  
Min Wang ◽  
Wen Niu ◽  
Yidan Wang ◽  
...  
Keyword(s):  
Wound Healing ◽  
Wound Repair ◽  
Skin Wound ◽  
Scavenging Activity ◽  
Self Healing ◽  
Nanocomposite Hydrogel ◽  
Full Thickness ◽  
Ros Scavenging ◽  
Full Thickness Skin ◽  
Thickness Skin

Abstract Excessive reactive oxygen species (ROS) in the injured skin may impede the wound repair and skin regeneration. Herein, we develop an injectable self-healing ceria-based nanocomposite hydrogel with ROS-scavenging activity to accelerate wound healing. The nanocomposite hydrogels were successfully prepared by coating cerium oxide nanorods with polyethylenimine (PEI) and crosslinked with benzaldehyde-terminated F127 (F127-CHO) through the dynamic Schiff-base reaction (FVEC hydrogel). The results showed that the FVEC hydrogel possessed the good thermosensitivity, injectability, self-healing ability and ROS scavenging activity. The subcutaneous implantation experiments in mice confirmed that FVEC hydrogels are biocompatible and biodegradable in vivo. The full-thickness skin wound studies showed that FVEC hydrogel could significantly enhance the wound healing and epithelium regeneration with the formation of hair follicle and adipocyte tissue. This work provides a new strategy for the development of multifunctional Ce-based nanocomposite hydrogel for full-thickness skin wound healing and regeneration.

Effect of locally injected autologous platelet-rich plasma on second intention wound healing of acute full-thickness skin defects in dogs

2015 ◽  
Vol 28 (03) ◽  
pp. 172-178 ◽  
Author(s):  
D. Psalla ◽  
G. Kazakos ◽  
P. Loukopoulos ◽  
N. Giannakas ◽  
I. Savvas ◽  
...  
Keyword(s):  
Wound Healing ◽  
Platelet Rich Plasma ◽  
Tissue Perfusion ◽  
Histological Evaluation ◽  
Full Thickness ◽  
Doppler Flowmetry ◽  
Skin Defects ◽  
Full Thickness Skin ◽  
Thickness Skin ◽  
Advanced Therapies

SummaryObjectives: Second intention wound healing may be impaired by wound and host factors and thus more advanced therapies are required for a fast and satisfactory outcome. Platelet-rich plasma (PRP), rich in growth factors and cytokines essential for tissue repair, could improve wound healing. The purpose of this experimental study was to evaluate the effect of locally injected autologous PRP on second intention healing of acute full-thickness skin defects in dogs.Methods: Three 2x2 cm full-thickness skin defects were created bilaterally on the dorso-lateral area of the trunk of six Beagle dogs. The wounds of one randomly selected side received PRP treatment, whereas the contra-lateral wounds were left untreated (controls). Wound healing was evaluated by planimetry, laser-Doppler flowmetry measurements of tissue perfusion, and histo-logically.Results: The rate of wound healing did not differ significantly between the two groups. Tissue perfusion was significantly higher in the PRP-treated group (p = 0.008) compared to controls on day 10. Histological evaluation revealed a trend towards greater collagen production and a significantly better collagen orientation (p = 0.019) in PRP-treated wounds on day 20.Clinical significance: Locally injected auto-logous PRP does not accelerate the healing process, but increases tissue perfusion and may promote the formation of organized collagen bundles in acute full-thickness skin defects in dogs.

Early wound repair versus later scar repair in children with treadmill hand friction burns

2021 ◽  
Author(s):  
Rong Zhou ◽  
Lin Qiu ◽  
Jun Xiao ◽  
Xiaobo Mao ◽  
Xingang Yuan
Keyword(s):  
Skin Graft ◽  
Wound Repair ◽  
Full Thickness ◽  
Full Thickness Skin Graft ◽  
Vacuum Sealing ◽  
Full Thickness Skin ◽  
Thickness Skin ◽  
Vacuum Sealing Drainage ◽  
Repair Group ◽  
Hand Friction

Abstract The incidence of pediatric treadmill hand friction burns has been increasing every year. The injuries are deeper than thermal hand burns, the optimal treatment remains unclear. This was a retrospective study of children who received surgery for treadmill hand friction burns from January 1, 2015, to December 31, 2019, in a single burn center. A total of 22 children were surveyed. The patients were naturally divided into two groups: the wound repair group (13 patients), which was admitted early to the hospital after injury and received debridement and vacuum sealing drainage initially, and a full-thickness skin graft later; and the scar repair group (9 patients), in which a scar contracture developed as a result of wound healing and received scar release and skin grafting later. The Modified Michigan Hand Questionnaire score in the wound repair group was 116.31 ± 10.55, and the corresponding score in the scar repair group was 117.56 ± 8.85 (P>0.05), no statistically significant difference. The Vancouver Scar Scale score in the wound repair group was 4.15 ± 1.21, and the corresponding score in the scar repair group was 7.22 ± 1.09 (P<0.05). Parents were satisfied with the postoperative appearance and function of the hand. None in the two groups required secondary surgery. If the burns are deep second degree, third degree, or infected, early debridement, vacuum sealing drainage initially, and a full-thickness skin graft can obviously relieve pediatric pain, shorten the course of the disease, and restore the function of the hand as soon as possible.

scholarly journals Development of a Novel Pre-Vascularized Three-Dimensional Skin Substitute Using Blood Plasma Gel

2018 ◽  
Vol 27 (10) ◽  
pp. 1535-1547 ◽  
Author(s):  
Niann-Tzyy Dai ◽  
Wen-Shyan Huang ◽  
Fang-Wei Chang ◽  
Lin-Gwei Wei ◽  
Tai-Chun Huang ◽  
...  
Keyword(s):  
Wound Healing ◽  
Human Plasma ◽  
Three Dimensional ◽  
Skin Substitute ◽  
Strength Analysis ◽  
Full Thickness ◽  
Skin Substitutes ◽  
Full Thickness Skin ◽  
Post Surgery ◽  
Thickness Skin

Skin substitutes with existing vascularization are in great demand for the repair of full-thickness skin defects. In the present study, we hypothesized that a pre-vascularized skin substitute can potentially promote wound healing. Novel three-dimensional (3D) skin substitutes were prepared by seeding a mixture of human endothelial progenitor cells (EPCs) and fibroblasts into a human plasma/calcium chloride formed gel scaffold, and seeding keratinocytes onto the surface of the plasma gel. The capacity of the EPCs to differentiate into a vascular-like tubular structure was evaluated using immunohistochemistry analysis and WST-8 assay. Experimental studies in mouse full-thickness skin wound models showed that the pre-vascularized gel scaffold significantly accelerated wound healing 7 days after surgery, and resembled normal skin structures after 14 days post-surgery. Histological analysis revealed that pre-vascularized gel scaffolds were well integrated in the host skin, resulting in the vascularization of both the epidermis and dermis in the wound area. Moreover, mechanical strength analysis demonstrated that the healed wound following the implantation of the pre-vascularized gel scaffolds exhibited good tensile strength. Taken together, this novel pre-vascularized human plasma gel scaffold has great potential in skin tissue engineering.

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