Micellar liquid chromatographic determination of salbutamol sulfate in presence of methyl paraben, propyl paraben and benzoic acid: application to content uniformity testing

2015 ◽  
Vol 12 (8) ◽  
pp. 1439-1446 ◽  
Author(s):  
Maha M. Abdelrahman
Keyword(s):  
Benzoic Acid ◽  
Chromatographic Determination ◽  
Content Uniformity ◽  
Salbutamol Sulfate ◽  
Methyl Paraben ◽  
Propyl Paraben ◽  
Liquid Chromatographic Determination ◽  
Content Uniformity Testing ◽  
Liquid Chromatographic

Liquid Chromatographic Determination of Coumarin Anticoagulants in Tablets: Collaborative Study

1987 ◽  
Vol 70 (5) ◽  
pp. 834-836
Author(s):  
Ella S Moore
Keyword(s):  
Mobile Phase ◽  
Collaborative Study ◽  
Chromatographic Determination ◽  
Content Uniformity ◽  
Photometric Detection ◽  
Warfarin Sodium ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract A liquid chromatographic method for the determination of coumarin anticoagulants in tablets was collaboratively studied by 7 laboratories. The method uses an octadecylsilane-bonded microparticulate column, tetrahydrofuran-methanol-water-acetic acid mobile phase, and photometric detection at 311 nm. Each collaborator received samples of warfarin sodium, phenprocoumon, and dicumarol as a synthetic composite and as commercial individual and composited tablets. Pooled average assay values for synthetic and commercial tablet samples of warfarin sodium were 101.6 and 99.5%, respectively, with a combined reproducibility SD of 2.38% (CV = 2.37%) and combined repeatability SD of 1.49% (CV = 1.49%). Pooled average (SD) assay values for dicumarol and phenprocoumon commercial samples were 98.0 (2.27) and 101.3% (4.00), respectively. The content uniformity determinations of 2 mg warfarin sodium and 25 mg dicumarol tablets indicated average tablet contents (range) of 99.5% (91.0-116.0) and 98.0% (89.8-108.8), respectively. The method has been approved interim official first action

Reverse Phase Liquid Chromatographic Determination of Bisacodyl in Dosage Forms

1985 ◽  
Vol 68 (3) ◽  
pp. 529-532
Author(s):  
Leonard P Valenti ◽  
Cesar A Lau-Cam
Keyword(s):  
Degradation Products ◽  
Chromatographic Determination ◽  
Content Uniformity ◽  
Liquid Chromatograph ◽  
Ultraviolet Detector ◽  
Liquid Chromatographic Determination ◽  
Phase Liquid ◽  
Enteric Coated ◽  
Liquid Chromatographic

Abstract A method is described for the determination of bisacodyl in entericcoated tablets and suppositories by liquid chromatography (LC). The method will also determine the hydrolysis degradation products monoacetylbisacodyl and desacetylbisacodyl. The sample is dissolved in 2- propanol, and the extract is diluted with the mobile phase and injected into a liquid chromatograph fitted with a μBondapak C18 column and an ultraviolet detector set at 254 nm. The column is eluted with methanol- acetonitrile-0.01M citric acid (25 + 25 + 50). The pooled mean recovery value for bisacodyl from commercial enteric-coated tablets and suppositories was 99.7 % with a pooled coefficient of variation (CV) of 0.72%. For content uniformity assays, the CVs were 0.7 and 1.0% for groups of 10 individual commercial suppositories and tablets, respectively. Differences between assay values by the LC and USP XX methods were 0.2% of declared for enteric-coated tablets (n = 5) and 1.0% of declared for suppositories (n = 2). The LC method can determine as little as 0.015 μg of the monoacetyl or desacetyl degradation product.

Liquid Chromatographic Determination of Coumarin Anticoagulants in Tablets

1986 ◽  
Vol 69 (4) ◽  
pp. 629-632
Author(s):  
Ella Moore ◽  
Cesar Lau-Cam
Keyword(s):  
Chromatographic Determination ◽  
Content Uniformity ◽  
Qualitative And Quantitative ◽  
Photometric Detection ◽  
Warfarin Sodium ◽  
Coefficients Of Variation ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract A simple and rapid liquid chromatographic method is described for the qualitative and quantitative determination of 5 coumarin anticoagulants in tablet composites and individual tablets. Analyses are carried out on a C18 reverse phase column using tetrahydrofuran-methanol-water-acetic acid (35 + 10 + 65 + 0.1) as mobile phase and photometric detection at 311 nm. The coefficients of variation for 10 consecutive injections of a mixed standards solution ranged from 0.28% for ethyl biscoumacetate to 0.78% for acenocoumarol. Standard recoveries were as follows: acenocoumarol, 99.3%; dicumarol, 99.6%; phenprocoumon, 101.6%; and warfarin sodium, 99.0%. The method was linear between 2 and 8 μg of drug injected. Assay results agreed favorably with those of the USP XX methods for dicumarol, phenprocoumon, and warfarin, and the NF XIV method for acenocoumarol. In addition, close correspondence was found with the results previously reported for the same drugs by a semiautomated spectrophotometric method. The content uniformity testing of individual 50 mg dicumarol tablets and 5 mg warfarin sodium tablets by the proposed method gave average (SD) values of 100.32% (0.64) and 101.00% (0.14), respectively, whereas these values were 101.60% (1.81) and 101.80% (0.18), respectively, by the method of USP XX.

Liquid Chromatographic Determination of Propyl Paraben in Cigarette Filler

1988 ◽  
Vol 71 (6) ◽  
pp. 1115-1117
Author(s):  
Jorge I CastaÑo ◽  
Francisco J Palacio ◽  
Luis R Vargas
Keyword(s):  
Chromatographic Determination ◽  
Cigarette Tobacco ◽  
Propyl Paraben ◽  
Coefficients Of Variation ◽  
Water Ph ◽  
Liquid Chromatographic Determination ◽  
Phase Liquid ◽  
Cut Tobacco ◽  
Liquid Chromatographic

Abstract A liquid chromatographic method for the quantitative determination of propyl paraben in cigarette tobacco filler has been developed. Propyl paraben is extracted from cigarette tobacco filler with acetonitrile and further purified using a silica Sep-Pak® cartridge and ethyl acetate-petroleum ether (1 + 4) as eluting solvent. The purified extracts are analyzed by reverse-phase liquid chromatography using buffered water (pH 4)-acetonitrile (65 + 35) as mobile phase, with UV detection at 254 nm. Cut tobacco samples were fortified with 100 and 200 ppm propyl paraben. Average recoveries (N = 5) of propyl paraben were 98 and 94%, respectively, with coefficients of variation less than 4%.

scholarly journals Liquid Chromatographic Determination of Thalidomide in Tablets, Capsules, and Raw Materials

1997 ◽  
Vol 80 (4) ◽  
pp. 767-774 ◽  
Author(s):  
John C Reepmeyer ◽  
Don C Cox
Keyword(s):  
Raw Materials ◽  
Internal Standard ◽  
Chromatographic Determination ◽  
Assay Method ◽  
Content Uniformity ◽  
Liquid Chromatographic Method ◽  
Bonded Phase ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract A simple, isocratic liquid chromatographic method for assay of thalidomide in tablets, capsules, and raw materials was developed. The method uses a Nova-Pak octadecylsilane bonded-phase column (150 × 3.9 mm, 4 μm particle size), a mobile phase of acetonitrile-water (15 + 85), a flow rate of 1 mL/min, detection at 237 nm, and phenacetin as internal standard. Phosphoric acid was used in preparation of sample solutions to inhibit thalidomide hydrolysis. Assays ranged from 99.3 to 100.4% in raw materials from 4 manufacturers, from 79.7 to 104.8% in tablets from 7 manufacturers, and from 75.3 to 102.6% in capsules from 4 manufacturers. Assay method precisions for triplicate analyses on 5 days were 0.30% for tablets, 0.22% for capsules, and 0.22% for raw materials. Recovery from simulated tablet formulations was 100%. The method has been used to analyze individual tablets and capsules for determination of content uniformity.

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