An enzymatic performance for a new swift magnetically detachable bio-conjugate of Candida rugosa lipase with modified Fe3O4–graphene oxide nanocomposite

2019 ◽  
Vol 17 (2) ◽  
pp. 367-382
Author(s):  
Faranak Jafarian ◽  
Abdol-Khalegh Bordbar ◽  
Atefeh Zare ◽  
Esmaeil Shams-Solari
Keyword(s):  
Graphene Oxide ◽  
Candida Rugosa ◽  
Candida Rugosa Lipase

scholarly journals Operational Stability, Regenerability, and Thermodynamics Studies on Biogenic Silica/Magnetite/Graphene Oxide Nanocomposite-Activated Candida rugosa Lipase

Polymers ◽  
2021 ◽  
Vol 13 (21) ◽  
pp. 3854
Author(s):  
Adikwu Gowon Jacob ◽  
Roswanira Abdul Wahab ◽  
Mailin Misson
Keyword(s):  
Graphene Oxide ◽  
Photoelectron Spectroscopy ◽  
Biogenic Silica ◽  
High Surface ◽  
Candida Rugosa ◽  
Candida Rugosa Lipase ◽  
Operational Stability ◽  
Proton Nuclear Magnetic Resonance ◽  
Hydrogen Atoms ◽  
X Ray

Inorganic biopolymer-based nanocomposites are useful for stabilizing lipases for enhanced catalytic performance and easy separation. Herein, we report the operational stability, regenerability, and thermodynamics studies of the ternary biogenic silica/magnetite/graphene oxide nanocomposite (SiO2/Fe3O4/GO) as a support for Candida rugosa lipase (CRL). The X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD), field-electron scanning electron microscopy (FESEM), vibrating sample magnetometry (VSM), and nitrogen adsorption/desorption data on the support and biocatalyst corroborated their successful fabrication. XPS revealed the Fe3O4 adopted Fe2+ and Fe3+ oxidation states, while XRD data of GO yielded a peak at 2θ = 11.67°, with the SiO2/Fe3O4/GO revealing a high surface area (≈261 m2/g). The fourier transform infrared (FTIR) spectra affirmed the successful fabricated supports and catalyst. The half-life and thermodynamic parameters of the superparamagnetic immobilized CRL (CRL/SiO2/Fe3O4/GO) improved over the free CRL. The microwave-regenerated CRL/SiO2/Fe3O4/GO (≈82%) exhibited higher catalytic activity than ultrasonic-regenerated (≈71%) ones. Lower activation (Ea) and higher deactivation energies (Ed) were also noted for the CRL/SiO2/Fe3O4/GO (13.87 kJ/mol, 32.32 kJ/mol) than free CRL (15.26 kJ/mol, 27.60 kJ/mol). A peak at 4.28 min in the gas chromatograph-flame ionization detection (GC-FID) chromatogram of the purified ethyl valerate supported the unique six types of 14 hydrogen atoms of the ester (CAS: 539-82-2) in the proton nuclear magnetic resonance (1H-NMR) data. The results collectively demonstrated the suitability of SiO2/Fe3O4/GO in stabilizing CRL for improved operational stability and thermodynamics and permitted biocatalyst regenerability.

Candida rugosa Lipase-Mediated Enantioselective Acetylation Studies on (.+-.)-3-Arylmethyl-3-hydroxymethyl-2,3-dihydro-1-benzopyran-4(H)-ones.

ChemInform ◽  
2005 ◽  
Vol 36 (23) ◽  
Author(s):  
Smriti Trikha ◽  
Rajesh Kumar ◽  
Ashish Dhawan ◽  
Poonam Poonam ◽  
Ashok K. Prasad ◽  
...  
Keyword(s):  
Candida Rugosa ◽  
Candida Rugosa Lipase
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