Immobilization of Candida rugosa lipase onto graphene oxide Fe 3 O 4 nanocomposite: Characterization and application for biodiesel production

2018 ◽  
Vol 159 ◽  
pp. 42-53 ◽  
Author(s):  
Wenlei Xie ◽  
Mengyun Huang
Keyword(s):  
Graphene Oxide ◽  
Candida Rugosa ◽  
Candida Rugosa Lipase ◽  
Biodiesel Production

An enzymatic performance for a new swift magnetically detachable bio-conjugate of Candida rugosa lipase with modified Fe3O4–graphene oxide nanocomposite

2019 ◽  
Vol 17 (2) ◽  
pp. 367-382
Author(s):  
Faranak Jafarian ◽  
Abdol-Khalegh Bordbar ◽  
Atefeh Zare ◽  
Esmaeil Shams-Solari
Keyword(s):  
Graphene Oxide ◽  
Candida Rugosa ◽  
Candida Rugosa Lipase

Candida rugosa lipase for the biodiesel production from renewable sources

2020 ◽  
Vol 162 ◽  
pp. 124-133
Author(s):  
Mariagrazia Iuliano ◽  
Maria Sarno ◽  
Salvatore De Pasquale ◽  
Eleonora Ponticorvo
Keyword(s):  
Candida Rugosa ◽  
Candida Rugosa Lipase ◽  
Biodiesel Production ◽  
Renewable Sources

scholarly journals Operational Stability, Regenerability, and Thermodynamics Studies on Biogenic Silica/Magnetite/Graphene Oxide Nanocomposite-Activated Candida rugosa Lipase

Polymers ◽  
2021 ◽  
Vol 13 (21) ◽  
pp. 3854
Author(s):  
Adikwu Gowon Jacob ◽  
Roswanira Abdul Wahab ◽  
Mailin Misson
Keyword(s):  
Graphene Oxide ◽  
Photoelectron Spectroscopy ◽  
Biogenic Silica ◽  
High Surface ◽  
Candida Rugosa ◽  
Candida Rugosa Lipase ◽  
Operational Stability ◽  
Proton Nuclear Magnetic Resonance ◽  
Hydrogen Atoms ◽  
X Ray

Inorganic biopolymer-based nanocomposites are useful for stabilizing lipases for enhanced catalytic performance and easy separation. Herein, we report the operational stability, regenerability, and thermodynamics studies of the ternary biogenic silica/magnetite/graphene oxide nanocomposite (SiO2/Fe3O4/GO) as a support for Candida rugosa lipase (CRL). The X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD), field-electron scanning electron microscopy (FESEM), vibrating sample magnetometry (VSM), and nitrogen adsorption/desorption data on the support and biocatalyst corroborated their successful fabrication. XPS revealed the Fe3O4 adopted Fe2+ and Fe3+ oxidation states, while XRD data of GO yielded a peak at 2θ = 11.67°, with the SiO2/Fe3O4/GO revealing a high surface area (≈261 m2/g). The fourier transform infrared (FTIR) spectra affirmed the successful fabricated supports and catalyst. The half-life and thermodynamic parameters of the superparamagnetic immobilized CRL (CRL/SiO2/Fe3O4/GO) improved over the free CRL. The microwave-regenerated CRL/SiO2/Fe3O4/GO (≈82%) exhibited higher catalytic activity than ultrasonic-regenerated (≈71%) ones. Lower activation (Ea) and higher deactivation energies (Ed) were also noted for the CRL/SiO2/Fe3O4/GO (13.87 kJ/mol, 32.32 kJ/mol) than free CRL (15.26 kJ/mol, 27.60 kJ/mol). A peak at 4.28 min in the gas chromatograph-flame ionization detection (GC-FID) chromatogram of the purified ethyl valerate supported the unique six types of 14 hydrogen atoms of the ester (CAS: 539-82-2) in the proton nuclear magnetic resonance (1H-NMR) data. The results collectively demonstrated the suitability of SiO2/Fe3O4/GO in stabilizing CRL for improved operational stability and thermodynamics and permitted biocatalyst regenerability.

scholarly journals Candida rugosa lipase nanoparticles as robust catalyst for biodiesel production in organic solvents

2019 ◽  
Vol 6 (3) ◽  
pp. 1025-1038 ◽  
Author(s):  
Rohit Kumar Sharma ◽  
Crystal A. O'Neill ◽  
Hector A.R. Ramos ◽  
Bibek Thapa ◽  
Vanessa C. Barcelo-Bovea ◽  
...  
Keyword(s):  
Organic Solvents ◽  
Candida Rugosa ◽  
Candida Rugosa Lipase ◽  
Biodiesel Production

scholarly journals Interesterifikasi minyak kelapa sawit dengan metil asetat untuk sintesis biodiesel menggunakan candida rugosa lipase terimobilisasi

2018 ◽  
Vol 8 (1) ◽  
pp. 24 ◽  
Author(s):  
Heri Hermansyah ◽  
Septian Marno ◽  
Rita Arbianti ◽  
Tania Surya Utami ◽  
Anondho Wijanarko
Keyword(s):  
Palm Oil ◽  
Methyl Acetate ◽  
Batch Reactor ◽  
Candida Rugosa ◽  
Candida Rugosa Lipase ◽  
Biodiesel Production ◽  
Reactant Ratio ◽  
Adsorption Method ◽  
Promising Alternative ◽  
Biodiesel Synthesis

Palm oil interesterification with methyl acetate for biodiesel synthesis using immobilized Candida rugosa lipaseBiocatalyst is a promising alternative catalyst for synthetic biodiesel because it has capability to improve conventional catalyst weakness, such as product purification and undesired side products. However, biocatalyst is easy to be deactivated by alcohol. Therefore, in this research, new method is developed to maintain the activity and stability of biocatalyst during reaction. In this paper, the experimental results of non-alcohol route synthesis of biodiesel using immobilized candida rugosa lipase in zeolit through adsorption method were reported. Methyl acetate as alkyl acceptor was reacted with triglyceride from palm oil in batch reactor. The analytical results from HPLC showed that trioleat convert up to 82% under the condition of 4%-wt substrate of the biocatalyst concentration and oil/alkyl mole ratio equal to 1/12 in 50 hour reaction. The effects of reactant ratio, biocatalyst concentration on concentration profile of tri-, di-, mono-gliceryde, and biodiesel were also observed. Stability test indicated that the activity of the immobilized biocatalyst still remained active for three reaction cycles.  Michaelis-Menten mechanism was used for derivation kinetic reaction equation to describe the behaviour of biodiesel production. Keywords: Biodiesel, interesterification, Candida rugosa lipase, non-alcohol route, immobilized. AbstrakSaat ini riset sintesis biodiesel menggunakan biokatalis sangat menjanjikan karena mampu memperbaiki kelemahan katalis alkali, yaitu kemudahan pemisahan produk dan kemampuan dalam mengarahkan reaksi secara spesifik tanpa adanya reaksi samping yang tidak diinginkan. Namun, biokatalis mudah terdeaktivasi dalam lingkungan beralkohol. Oleh karena itu, dalam riset ini diusulkan untuk melakukan sintesis biodiesel melalui rute non-alkohol untuk menjaga agar aktivitas dan stabilitas biokatalis tetap tinggi selama reaksi berlangsung. Dalam makalah ini akan disajikan hasil penelitian sintesis biodiesel rute non-alkohol menggunakan Candida rugosa lipase yang diimobilisasi dalam zeolit melalui metode adsorpsi dengan mereaksikan metil asetat sebagai penyuplai gugus alkil dengan trigliserida dari minyak kelapa sawit dalam reaktor batch. Hasil analisis HPLC menunjukkan bahwa lebih dari 82% rantai asam lemak dari trigliserida minyak kelapa sawit berhasil dikonversikan menjadi biodiesel pada kondisi konsentrasi biokatalis sebesar 4%-wt substrat dan rasio mol minyak/alkil sebesar 1/12 selama 50 jam reaksi. Pengaruh rasio reaktan, konsentrasi biokatalis terhadap profil konsentrasi dari tri-, di-, mono-gliserida serta biodiesel juga diselidiki. Uji stabilitas menunjukkan bahwa biokatalis terimobilisasi ini masih memiliki aktivitas untuk tiga kali siklus reaksi. Mekanisme Michaelis-Menten digunakan untuk menurunkan persamaan kinetika reaksi yang mampu menggambarkan perilaku produksi biodiesel yang dihasilkan.Kata kunci: biodiesel, interesterifikasi, Candida rugosa lipase, rute non alkohol, imobilisasi

Biodiesel Production from Waste Cooking Oil by Immobilized Candida rugosa Lipase in PVA-Alginate-Sulfate Beads

2018 ◽  
Vol 24 (5) ◽  
pp. 3658-3663
Author(s):  
Abdulkarim Ali Deba ◽  
Nor Azimah Mohd Zain ◽  
Nor Badzilah Hasan ◽  
Idris Adamu Matinjaa
Keyword(s):  
Candida Rugosa ◽  
Candida Rugosa Lipase ◽  
Waste Cooking Oil ◽  
Biodiesel Production ◽  
Cooking Oil ◽  
Immobilized Candida Rugosa Lipase
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