Evaluation of extreme resistance genes of Potato virus X (Rx1 and Rx2) in different potato genotypes

The genotypes of potatoes bred by Falenki Breeding station were studied for the presence of resistance genes markers to the following pathogens: Globodera rostochiensis, Globodera pallidа, Synchytrium endobioticum, potato virus X (PVХ) and potato virus Y (PVY). The method of multiplex PCR analysis was used. The varieties Shurminsky 2, Alisa, Viza, Chayka, Ognivo, Darik, Gloriya, Golubka, Virazh and a promising variety sample 56-09 were studied. In most (8 out of 10) genotypes, marker linked to the Sen1 gene of resistance to S. endobioticum was identified. DNA marker of the G. rostochiensis resistance gene (H1) and the G. pallida resistance gene marker (Gpa2) were found in six genotypes. The marker of the PVX resistance gene (Rx1) was detected in the varieties Shurminsky 2, Alisa, Chayka, Golubka, and Virazh. It has been established that none of the studied potato genotypes carries markers RYSC3, Ry186, YES3-3A linked to the PVY resistance genes. Although in the field, resistance was detected in the samples Chayka, Darik, Virazh, Alisa. Molecular markers linked to the largest number of resistance genes studied (H1, Gpa2, Sen1, and Rx1) were identified in the varieties Shurminsky 2, Golubka, and Virazh. Among the DNA markers used in the work, the data of potato genotype assessment using markers of virus resistance genes (PVX, RYSC3, Ry186, YES3-3A) were less consistent with field observations. The use of molecular markers makes it possible to determine the presence of resistance genes and assess the prospects of a sample in a short period of time, but, at the same time, requires careful choice of a DNA marker that is highly correlated with the manifestation of the trait.

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Gene-centromere mapping in potato by half-tetrad analysis: map distances of H1, Rx, and Ry and their possible use for ascertaining the mode of 2n-pollen formation

Genome ◽

10.1139/g92-001 ◽

1992 ◽

Vol 35 (1) ◽

pp. 1-7 ◽

Cited By ~ 6

Author(s):

Marinus Wagenvoort ◽

Ewa Zimnoch-Guzowska

Keyword(s):

Potato Virus ◽

Potato Virus Y ◽

Potato Virus X ◽

2N Pollen ◽

Tetrad Analysis ◽

Extreme Resistance ◽

Second Division Restitution ◽

Pollen Formation ◽

Tetraploid Progeny ◽

Map Distance

Diploids from the tetraploid potato varieties 'Alcmaria' and 'Pansta' and from the tetraploid CPRO genotypes Y66-13-610 and Y66-13-636 were used in half-tetrad analyses to estimate the gene-centromere map distances of the genes Rx, Ry, and H1. Employing tetraploid progeny from 2x (second division restitution) – 4x testcrosses the gene-centromere map distance of H1, conferring resistance to pathotype Ro1 of Globodera rostochiensis, was estimated to be 16.3 centimorgans (cM). For Rχ, conferring extreme resistance to potato virus X (PVX), a map distance of 33.9 cM was estimated. The gene Ry, conferring extreme resistance to potato virus Y (PVY), was estimated to be located 14.2 cM from the centromere. Using the estimated map distance for Rx, it was attempted to determine the mode of 2n-pollen formation in four diploid interspecific hybrids, including the species Solanum tuberosum, Solanum chacoense, Solanum yungasense, and Solanum phureja, by half-tetrad analysis in tetraploid progeny from 4x-2x testcrosses. The mean frequency of 8.7% nulliplex plants for Rx was outside the range of the 95% confidence intervals, for both first division restitution and second division restitution 2n pollen.Key words: nematode resistance, potato virus X resistance, potato virus Y resistance, 2n eggs, gene-centromere mapping, Solanum.

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Occurrence of potato virus X strain group 1 in seed stocks of potato cultivars lacking resistance genes

Annals of Applied Biology ◽

10.1111/j.1744-7348.1995.tb07606.x ◽

1995 ◽

Vol 127 (3) ◽

pp. 479-487 ◽

Cited By ~ 7

Author(s):

C R. Wilson ◽

R A C. Jones

Keyword(s):

Potato Virus ◽

Resistance Genes ◽

Potato Virus X ◽

Potato Cultivars ◽

Strain Group ◽

Group 1

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Extreme resistance to infection by potato virus X in genotypes of wild tuber-bearingSolanum species

Potato Research ◽

10.1007/bf02360505 ◽

1991 ◽

Vol 34 (3) ◽

pp. 317-324 ◽

Cited By ~ 20

Author(s):

Alejandro Carlos Tozzini ◽

María Fernanda Ceriani ◽

María Verónica Saladrigas ◽

H. Esteban Hopp

Keyword(s):

Potato Virus ◽

Potato Virus X ◽

Extreme Resistance ◽

Resistance To Infection

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High-resolution genetical and physical mapping of the Rx gene for extreme resistance to potato virus X in tetraploid potato

Theoretical and Applied Genetics ◽

10.1007/s001220050543 ◽

1997 ◽

Vol 95 (1-2) ◽

pp. 153-162 ◽

Cited By ~ 55

Author(s):

A. Bendahmane ◽

K. Kanyuka ◽

D. C. Baulcombe

Keyword(s):

High Resolution ◽

Potato Virus ◽

Physical Mapping ◽

Potato Virus X ◽

Extreme Resistance ◽

Tetraploid Potato

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Yukon Nugget: a Mid-Season Yellow Skin, Yellow Flesh Specialty Potato with Extreme Resistance to Potato Virus X

American Journal of Potato Research ◽

10.1007/s12230-016-9540-6 ◽

2016 ◽

Vol 93 (6) ◽

pp. 602-608 ◽

Cited By ~ 3

Author(s):

V. Sathuvalli ◽

C. R. Brown ◽

S. Yilma ◽

B. A. Charlton ◽

C. C. Shock ◽

...

Keyword(s):

Potato Virus ◽

Potato Virus X ◽

Extreme Resistance ◽

Yellow Flesh

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Biological Properties of Potato virus X in Potato: Effects of Mixed Infection with Potato virus S and Resistance Phenotypes in Cultivars from Three Continents

Plant Disease ◽

10.1094/pdis-04-11-0305 ◽

2012 ◽

Vol 96 (1) ◽

pp. 43-54 ◽

Cited By ~ 17

Author(s):

Eviness P. Nyalugwe ◽

Calum R. Wilson ◽

Brenda A. Coutts ◽

Roger A. C. Jones

Keyword(s):

Potato Virus ◽

Resistance Genes ◽

Mixed Infection ◽

North American ◽

Potato Virus X ◽

Biological Properties ◽

Breeding Line ◽

Potato Virus S ◽

Enhanced Expression ◽

First Time

Interactions between Potato virus X (PVX) and Potato virus S (PVS) were studied in potato plants, and isolates of PVX were inoculated to potato cultivars from four continents to identify occurrence of PVX resistance genes. Mixed infection with PVX and PVS increased the titer of PVS and enhanced expression of foliar symptoms in primarily and secondarily infected plants of ‘Royal Blue’. PVX isolates belonging to strain groups 1 and 3 (WA1+3) or 3 (XK3 and TAS3) were sap and graft inoculated (1 to 3 isolates each) to 38 cultivars and one breeding line. Presence of extreme PVX resistance gene Rx was identified in four Australian (‘Auski’, ‘Billabong’, ‘Flame’, and ‘Ruby Lou’) and two European (‘Mondial’ and ‘Rodeo’) cultivars, and in a clone of North American ‘Atlantic’. PVX hypersensitivity gene Nx was identified for the first time in two Australian (‘Bliss’ and ‘MacRusset’), four European (‘Almera’, ‘Harmony’, ‘Maxine’, and ‘Nadine’), and one North American (‘Ranger Russet’) cultivars, and in Australian breeding line 98-10713. PVX hypersensitivity gene Nb was identified for the first time in one Australian (‘White Star’), five European (‘Innovator’, ‘Kestrel’, ‘Kipfler’, ‘Laurine’, and ‘Royal Blue’), and one North American (‘Shepody’) cultivars. Probable ancestral sources of the resistance genes found in different cultivars were identified. Thus, although PVX resistance genes often occur in parents used in crosses, knowledge of their occurrence in parents and cultivars is often lacking. On sap inoculation, systemic hypersensitive phenotypes that caused shoot death often developed in cultivars with Nx but not necessarily in all shoots. This phenotype caused severe necrotic symptoms in infected tubers. In some instances, passage through cultivars with Nb separated strain group 3 from mixed isolate WA1+3.

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