Gene-centromere mapping in potato by half-tetrad analysis: map distances of H1, Rx, and Ry and their possible use for ascertaining the mode of 2n-pollen formation

Diploids from the tetraploid potato varieties 'Alcmaria' and 'Pansta' and from the tetraploid CPRO genotypes Y66-13-610 and Y66-13-636 were used in half-tetrad analyses to estimate the gene-centromere map distances of the genes Rx, Ry, and H1. Employing tetraploid progeny from 2x (second division restitution) – 4x testcrosses the gene-centromere map distance of H1, conferring resistance to pathotype Ro1 of Globodera rostochiensis, was estimated to be 16.3 centimorgans (cM). For Rχ, conferring extreme resistance to potato virus X (PVX), a map distance of 33.9 cM was estimated. The gene Ry, conferring extreme resistance to potato virus Y (PVY), was estimated to be located 14.2 cM from the centromere. Using the estimated map distance for Rx, it was attempted to determine the mode of 2n-pollen formation in four diploid interspecific hybrids, including the species Solanum tuberosum, Solanum chacoense, Solanum yungasense, and Solanum phureja, by half-tetrad analysis in tetraploid progeny from 4x-2x testcrosses. The mean frequency of 8.7% nulliplex plants for Rx was outside the range of the 95% confidence intervals, for both first division restitution and second division restitution 2n pollen.Key words: nematode resistance, potato virus X resistance, potato virus Y resistance, 2n eggs, gene-centromere mapping, Solanum.

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Extreme resistance to infection by potato virus Y and potato virus X in an advanced hybridSolanum phureja — S. Stenotomum diploid potato population

American Potato Journal ◽

10.1007/bf02851431 ◽

1994 ◽

Vol 71 (10) ◽

pp. 617-628 ◽

Cited By ~ 5

Author(s):

Roger L. Vallejo ◽

Wanda W. Collins ◽

Rocco D. Schiavone ◽

Steven A. Lommel ◽

J. B. Young

Keyword(s):

Potato Virus ◽

Potato Virus Y ◽

Potato Virus X ◽

Diploid Potato ◽

Extreme Resistance ◽

Resistance To Infection

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Perturbation of nuclear‐cytosolic shuttling of Rx1 compromises extreme resistance and translational arrest of potato virus X transcripts

The Plant Journal ◽

10.1111/tpj.15179 ◽

2021 ◽

Author(s):

Manon MS Richard ◽

Marijn Knip ◽

Joëlle Schachtschabel ◽

Machiel S Beijaert ◽

Frank LW Takken

Keyword(s):

Potato Virus ◽

Potato Virus X ◽

Extreme Resistance ◽

Translational Arrest

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Expression of symptoms, viral coat protein and silencing suppressor gene during mixed infection of a N–Wi strain of potato virus Y and an asymptomatic strain of potato virus X

VirusDisease ◽

10.1007/s13337-014-0204-1 ◽

2014 ◽

Vol 25 (3) ◽

pp. 314-321 ◽

Cited By ~ 7

Author(s):

D. M. J. B. Senanayake ◽

B. Mandal

Keyword(s):

Coat Protein ◽

Potato Virus ◽

Mixed Infection ◽

Potato Virus Y ◽

Potato Virus X ◽

Suppressor Gene ◽

Silencing Suppressor ◽

Viral Coat Protein ◽

Viral Coat

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Use of half-tetrad analysis to discriminate between two types of 2n egg formation in a potato haploid

Genome ◽

10.1139/g92-114 ◽

1992 ◽

Vol 35 (5) ◽

pp. 741-745 ◽

Cited By ~ 8

Author(s):

Joanna E. Werner ◽

David S. Douches ◽

Rosanna Freyre

Keyword(s):

Solanum Tuberosum L ◽

2N Gametes ◽

Breeding Value ◽

Tetrad Analysis ◽

Recombination Rates ◽

First Division Restitution ◽

Egg Formation ◽

Second Division Restitution ◽

2N Eggs ◽

Tetraploid Progeny

The ratio of the first division restitution (FDR) to second division restitution (SDR) 2n eggs was estimated in 4182t, a haploid (2n = 2x = 24) of Solanum tuberosum L. that produces 2n eggs by the two modes. The segregation of three genes previously mapped relative to their centromeres, Pgm-2 (2.0 cM), Mdh-1 (33.5 cM), and 6-Pgdh-3 (30.1 cM) was analyzed in the tetraploid offspring of a 2x × 4x cross. Based on the segregation of the Pgm-2 locus, 39.7% of the progeny originated from FDR 2n eggs and 60.3% from SDR. Segregation patterns of the two distal loci within the FDR-derived 4x subpopulation indicated that the gene–centromere recombination rate during megasporogenesis was significantly reduced for Mdh-1 when compared with a previous estimate during microsporogenesis. In the SDR-derived 4x subpopulation, the gene–centromere recombination rates for Mdh-1 and 6-Pgdh-3 were not significantly different from previous estimates. Tetraploid progeny generated from one 2x × 4x cross where the 2x parent produces 2n gametes by two modes can be used to make an unbiased comparison of the potential breeding value of FDR and SDR gametes.Key words: potato, megasporogenesis, first division restitution, second division restitution, isozyme.

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Epidemic of Potato virus Y and Cucumber mosaic virus in Henan Province Tobacco

Plant Disease ◽

10.1094/pdis.2001.85.4.447c ◽

2001 ◽

Vol 85 (4) ◽

pp. 447-447 ◽

Cited By ~ 1

Author(s):

X. D. Li ◽

Y. Q. Li ◽

H. G. Wang

Keyword(s):

Cucumber Mosaic Virus ◽

Mosaic Virus ◽

Potato Virus ◽

Potato Virus Y ◽

Early Stage ◽

Potato Virus X ◽

Henan Province ◽

Enzyme Linked Immunosorbent Assay ◽

Resistant Varieties ◽

Vein Necrosis

Flue-cured tobacco is an important crop in Henan Province, China. During the 2000 growing season, many tobacco plants showed various degrees of mottling, mosaic, vein clearing, or vein necrosis in most of the counties. Some plants even died at an early stage of growth. A survey was conducted in May-June in several tobacco-growing counties, and the incidence of symptomatic plants in individual fields ranged from 10 to 85%. The most widely planted tobacco varieties, NC89, K326, and K346, were highly susceptible. Symptomatic plants were collected from Jiaxian and Xiangcheng counties and samples were tested by enzyme-linked immunosorbent assay for Tobacco mosaic virus (TMV), Cucumber mosaic virus (CMV), Potato virus Y (PVY), and Potato virus X (PVX). Of 65 samples tested, 21 were positive for only PVY, 16 positive for only CMV, one each was positive for only TMV or PVX. Nineteen samples were doubly infected with various combinations of these viruses and six were infected with combinations of three viruses. The causal agent(s) in the remaining sample could not be determined. In total, CMV was detected in 40 samples, PVY in 38, PVX in 10, and TMV in 7 samples. TMV and CMV used to be the most important viruses and PVY occurred only rarely. But PVY has become prevalent in Henan and in neighboring Shandong province (2). CMV and TMV were reported to be the most prevalent viruses in Shanxi (1) and Fujian Provinces (3). Because resistant varieties are not available, and mixed infections are more common, the results presented here explain why huge damage is occurring in tobacco crops in recent years. Some varieties are partially resistant to TMV and CMV but the varieties commonly grown are highly susceptible to PVY. Therefore, breeding for resistance to viruses, especially to PVY, is urgent to control the occurrence of tobacco viral diseases. References: (1) J. L. Cheng et al. Acta Tabacaria Sin. 4:43, 1998. (2) J. B. Wang et al. Chinese Tobacco Sci. 1:26, 1998. (3) L. H. Xie et al. Acta Tabacaria Sin. 2:25, 1994.

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