Occurrence of potato virus X strain group 1 in seed stocks of potato cultivars lacking resistance genes

The genotypes of potatoes bred by Falenki Breeding station were studied for the presence of resistance genes markers to the following pathogens: Globodera rostochiensis, Globodera pallidа, Synchytrium endobioticum, potato virus X (PVХ) and potato virus Y (PVY). The method of multiplex PCR analysis was used. The varieties Shurminsky 2, Alisa, Viza, Chayka, Ognivo, Darik, Gloriya, Golubka, Virazh and a promising variety sample 56-09 were studied. In most (8 out of 10) genotypes, marker linked to the Sen1 gene of resistance to S. endobioticum was identified. DNA marker of the G. rostochiensis resistance gene (H1) and the G. pallida resistance gene marker (Gpa2) were found in six genotypes. The marker of the PVX resistance gene (Rx1) was detected in the varieties Shurminsky 2, Alisa, Chayka, Golubka, and Virazh. It has been established that none of the studied potato genotypes carries markers RYSC3, Ry186, YES3-3A linked to the PVY resistance genes. Although in the field, resistance was detected in the samples Chayka, Darik, Virazh, Alisa. Molecular markers linked to the largest number of resistance genes studied (H1, Gpa2, Sen1, and Rx1) were identified in the varieties Shurminsky 2, Golubka, and Virazh. Among the DNA markers used in the work, the data of potato genotype assessment using markers of virus resistance genes (PVX, RYSC3, Ry186, YES3-3A) were less consistent with field observations. The use of molecular markers makes it possible to determine the presence of resistance genes and assess the prospects of a sample in a short period of time, but, at the same time, requires careful choice of a DNA marker that is highly correlated with the manifestation of the trait.

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Evaluation of potato virus X resistance in potato cultivars and identification of an innate immunity-independent resistance phenotype

Phytopathology Research ◽

10.1186/s42483-021-00099-6 ◽

2021 ◽

Vol 3 (1) ◽

Author(s):

Jiahui Liu ◽

Ye Liu ◽

Yue Fang ◽

Lili Zhang ◽

Kaixin Yu ◽

...

Keyword(s):

Potato Virus ◽

Fluorescent Protein ◽

Infectious Clone ◽

Yellow Fluorescent Protein ◽

Potato Virus X ◽

Potato Production ◽

Potato Cultivars ◽

Major Type ◽

Long Distance ◽

Viral Pathogen

AbstractPotato virus X (PVX) is a widely distributed viral pathogen that causes significant losses in potato production by co-infecting with potato virus Y or potato virus A. In this study, the resistance of 23 potato cultivars to PVX was dissected in detail using a PVX infectious clone containing a yellow fluorescent protein (YFP). Among them, four potato cultivars (Longshu-3, Eugene, Atlantic and Waiyin-2) were found to carry an Rx gene that confers extreme resistance to PVX; one cultivar (Waiyin-1) displayed partial resistance and was able to delay PVX infection by ~ 5 days; while the rest eighteen potato cultivars were susceptible to PVX. Moreover, we found that the replication but not cell-to-cell or long-distance movement of PVX was inhibited in Waiyin-1. Finally, we determined that the expression of pathogenesis-related (PR) genes in Waiyin-1 was not triggered by PVX infection at early infection stage, whereas they were triggered in the Rx-carrying cultivar Atlantic during this period of time. In conclusion, our results confirm that Rx is a major type of resistance gene in potato cultivars in the Northeast part of China. Furthermore, the possible mechanism underlying Waiyin-1 resistance to PVX is discussed.

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Evaluation of extreme resistance genes of Potato virus X (Rx1 and Rx2) in different potato genotypes

Journal of Plant Diseases and Protection ◽

10.1007/s41348-018-0148-6 ◽

2018 ◽

Vol 125 (3) ◽

pp. 251-257 ◽

Cited By ~ 2

Author(s):

Hassan O. Shaikhaldein ◽

Borbala Hoffmann ◽

Ibrahim A. Alaraidh ◽

Dalia G. Aseel

Keyword(s):

Potato Virus ◽

Resistance Genes ◽

Potato Virus X ◽

Extreme Resistance

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The Genetic Engineering of Two Commercial Potato Cultivars for Resistance to Potato Virus X

Nature Biotechnology ◽

10.1038/nbt0389-273 ◽

1989 ◽

Vol 7 (3) ◽

pp. 273-278 ◽

Cited By ~ 58

Author(s):

André Hoekema ◽

Marianne J. Huisman ◽

Lucy Molendijk ◽

Peter J. M. van den Elzen ◽

Ben J. C. Cornelissen

Keyword(s):

Genetic Engineering ◽

Potato Virus ◽

Potato Virus X ◽

Potato Cultivars ◽

Commercial Potato

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Biological Properties of Potato virus X in Potato: Effects of Mixed Infection with Potato virus S and Resistance Phenotypes in Cultivars from Three Continents

Plant Disease ◽

10.1094/pdis-04-11-0305 ◽

2012 ◽

Vol 96 (1) ◽

pp. 43-54 ◽

Cited By ~ 17

Author(s):

Eviness P. Nyalugwe ◽

Calum R. Wilson ◽

Brenda A. Coutts ◽

Roger A. C. Jones

Keyword(s):

Potato Virus ◽

Resistance Genes ◽

Mixed Infection ◽

North American ◽

Potato Virus X ◽

Biological Properties ◽

Breeding Line ◽

Potato Virus S ◽

Enhanced Expression ◽

First Time

Interactions between Potato virus X (PVX) and Potato virus S (PVS) were studied in potato plants, and isolates of PVX were inoculated to potato cultivars from four continents to identify occurrence of PVX resistance genes. Mixed infection with PVX and PVS increased the titer of PVS and enhanced expression of foliar symptoms in primarily and secondarily infected plants of ‘Royal Blue’. PVX isolates belonging to strain groups 1 and 3 (WA1+3) or 3 (XK3 and TAS3) were sap and graft inoculated (1 to 3 isolates each) to 38 cultivars and one breeding line. Presence of extreme PVX resistance gene Rx was identified in four Australian (‘Auski’, ‘Billabong’, ‘Flame’, and ‘Ruby Lou’) and two European (‘Mondial’ and ‘Rodeo’) cultivars, and in a clone of North American ‘Atlantic’. PVX hypersensitivity gene Nx was identified for the first time in two Australian (‘Bliss’ and ‘MacRusset’), four European (‘Almera’, ‘Harmony’, ‘Maxine’, and ‘Nadine’), and one North American (‘Ranger Russet’) cultivars, and in Australian breeding line 98-10713. PVX hypersensitivity gene Nb was identified for the first time in one Australian (‘White Star’), five European (‘Innovator’, ‘Kestrel’, ‘Kipfler’, ‘Laurine’, and ‘Royal Blue’), and one North American (‘Shepody’) cultivars. Probable ancestral sources of the resistance genes found in different cultivars were identified. Thus, although PVX resistance genes often occur in parents used in crosses, knowledge of their occurrence in parents and cultivars is often lacking. On sap inoculation, systemic hypersensitive phenotypes that caused shoot death often developed in cultivars with Nx but not necessarily in all shoots. This phenotype caused severe necrotic symptoms in infected tubers. In some instances, passage through cultivars with Nb separated strain group 3 from mixed isolate WA1+3.

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Resistance to potato leafroll virus infection and accumulation in potato cultivars, and the effects of previous infection with other viruses on expression of resistance

Australian Journal of Agricultural Research ◽

10.1071/ar9931891 ◽

1993 ◽

Vol 44 (8) ◽

pp. 1891 ◽

Cited By ~ 24

Author(s):

CR Wilson ◽

RAC Jones

Keyword(s):

Potato Virus ◽

Potato Virus X ◽

Potato Leafroll Virus ◽

Potato Cultivars ◽

Infected Leaf ◽

Potato Virus S ◽

Breeding Lines ◽

Infection Pressure ◽

Previous Infection ◽

Leafroll Virus

A selection of potato cultivars and breeding lines was evaluated for presence of resistance to infection with potato leafroll virus (PLRV) via viruliferous aphid vectors ( IR) and/or resistance to accumulation of PLRV antigen ( AR) in infected leaf tissue. Cultivars Aracy, Delcora, Omega and Spunta, and breeding lines BR63.15 and B71.240.2 carried both IR and AR , Bismark, Serrana INTA and L/T1 had alone and Delaware had AR alone. The other cultivars tested had neither. Within both the resistant and susceptible classes for AR, the level of PLRV antigen accumulation achieved varied with cultivar. Previous infection with potato virus X (PVX) or potato virus S (PVS) either alone or together did not diminish the expression of IR or AR. However, the presence of PVX sometimes significantly increased the accumulation of PLRV in susceptible cv. Desiree and this effect was most pronounced in mature leaves of older plants. In contrast, presence of PVX in susceptible cv. Desiree did not increase the numbers of plants becoming infected with PLRV. Identifying potato genotypes with and IR AR will help the Australian potato industry to select cultivars which become infected with PLRV more slowly under conditions of high infection pressure and/or are suitable for use as parental lines in breeding virus-resistant cultivars.

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