Evaluation of a resorufin-based fluorescent probe for tyrosinase detection in skin pigmentation disorders

2021 ◽  
Author(s):  
Yibo Hu ◽  
Hongliang Zeng ◽  
Jiaxing Jiang ◽  
Sheng Yang ◽  
Jinhua Huang ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Skin Pigmentation ◽  
Pigmentation Disorders

scholarly journals Paracrine Secreted Frizzled-Related Protein 4 Inhibits Melanocytes Differentiation in Hair Follicle

2017 ◽  
Vol 2017 ◽  
pp. 1-12 ◽  
Author(s):  
Haiying Guo ◽  
Mingxing Lei ◽  
Yuhong Li ◽  
Yingxin Liu ◽  
Yinhong Tang ◽  
...  
Keyword(s):  
Wnt Signaling ◽  
Hair Follicle ◽  
Skin Pigmentation ◽  
Related Protein ◽  
Skin Cells ◽  
Melanocyte Stem Cells ◽  
Potential Therapeutic Application ◽  
Pigmentation Disorders

Wnt signaling plays crucial role in regulating melanocyte stem cells/melanocyte differentiation in the hair follicle. However, how the Wnt signaling is balanced to be overactivated to control follicular melanocytes behavior remains unknown. Here, by using immunofluorescence staining, we showed that secreted frizzled-related protein 4 (sFRP4) is preferentially expressed in the skin epidermal cells rather than in melanocytes. By overexpression of sFRP4 in skin cells in vivo and in vitro, we found that sFRP4 attenuates activation of Wnt signaling, resulting in decrease of melanocytes differentiation in the regenerating hair follicle. Our findings unveiled a new regulator that involves modulating melanocytes differentiation through a paracrine mechanism in hair follicle, supplying a hope for potential therapeutic application to treat skin pigmentation disorders.

scholarly journals Mechanisms regulating melanogenesis*

2013 ◽  
Vol 88 (1) ◽  
pp. 76-83 ◽  
Author(s):  
Inês Ferreira dos Santos Videira ◽  
Daniel Filipe Lima Moura ◽  
Sofia Magina
Keyword(s):  
Adrenocorticotropic Hormone ◽  
Skin Pigmentation ◽  
Phenotypic Trait ◽  
Extrinsic Factors ◽  
Melanocyte Stimulating Hormone ◽  
Complex Process ◽  
Central Nervous Systems ◽  
Gene Defects ◽  
Pigmentation Disorders

Skin pigmentation is an important human phenotypic trait whose regulation, in spite of recent advances, has not yet been fully understood. The pigment melanin is produced in melanosomes by melanocytes in a complex process called melanogenesis. The melanocyte interacts with endocrine, immune, inflammatory and central nervous systems, and its activity is also regulated by extrinsic factors such as ultraviolet radiation and drugs. We have carried out a review of the current understanding of intrinsic and extrinsic factors regulating skin pigmentation, the melanogenesis stages and related gene defects. We focused on melanocyte-keratinocyte interaction, activation of melanocortin type 1 receptor (MC1-R) by peptides (melanocyte-stimulating hormone and adrenocorticotropic hormone) resulting from proopiomelanocortin (POMC) cleavage, and mechanisms of ultraviolet-induced skin pigmentation. The identification and comprehension of the melanogenesis mechanism facilitate the understanding of the pathogenesis of pigmentation disorders and the development of potential therapeutic options.

Pigmentation disorders: types, aetiology and treatment options

2019 ◽  
Vol 8 (5) ◽  
pp. 235-239
Author(s):  
Rekha Tailor
Keyword(s):  
Skin Disorder ◽  
Detrimental Effect ◽  
Treatment Options ◽  
Skin Pigmentation ◽  
Different Types ◽  
Aesthetic Practice ◽  
Chronic Skin ◽  
Darker Skin Types ◽  
Pigmentation Disorders

Hyperpigmentation is a chronic skin disorder that can be very difficult to treat. Skin pigmentation disorders are commonly seen in women than in men, due to hormonal factors, and in those with darker skin types. Pigmentation disorders can cause significant stress for patients and can have a detrimental effect on their quality of life; it is therefore important that aesthetic practitioners have a sound understanding of the different types of hyperpigmentation and appropriate treatments. This article will explore pigmentation disorders and how they can be treated in aesthetic practice.

Skin disorders

2013 ◽  
Author(s):  
Julia Smedley ◽  
Finlay Dick ◽  
Steven Sadhra
Keyword(s):  
Skin Pigmentation ◽  
Skin Infections ◽  
Skin Disorders ◽  
Occupational Dermatitis ◽  
Contact Urticaria ◽  
Skin Cancers ◽  
The Uk ◽  
Pigmentation Disorders

Dermatitis 1 242Dermatitis 2: management 244Contact urticaria 246Skin cancers 247Skin pigmentation disorders 248Folliculitis and acne 249Photodermatitis 250Scleroderma 251Occupational skin infections 252Prevalence data1 suggest that 20000 people in the UK have skin problems that are caused or made worse by work. Occupational dermatitis (OD) makes up the greatest proportion of these. Data come from two main sources: ...

scholarly journals Milk Protein-Derived Antioxidant Tetrapeptides as Potential Hypopigmenting Agents

Antioxidants ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 1106
Author(s):  
Saerom Kong ◽  
Hye-Ryung Choi ◽  
Yoon-Jeong Kim ◽  
Yoon-Sik Lee ◽  
Kyoung-Chan Park ◽  
...  
Keyword(s):  
Milk Protein ◽  
Antioxidant Activities ◽  
Biological Activities ◽  
Skin Pigmentation ◽  
Milk Proteins ◽  
Mushroom Tyrosinase ◽  
Antioxidant Peptides ◽  
Β Lactoglobulin ◽  
Pigmentation Disorders ◽  
Excessive Accumulation

Excessive accumulation of melanin can cause skin pigmentation disorders, which may be accompanied by significant psychological stress. Although many natural and synthetic products have been developed for the regulation of melanogenesis biochemistry, the management of unwanted skin pigmentation remains challenging. Herein, we investigated the potential hypopigmenting properties of peptide sequences that originated from milk proteins such as ĸ-casein and β-lactoglobulin. These proteins are known to inhibit melanogenesis and their hydrolysates are reported as antioxidant peptides. We synthesize tetrapeptide fragments of the milk protein hydrolysates and investigate the amino acids that are essential for designing peptides with tyrosinase inhibitory and antioxidant activities. We found that the peptide methionine-histidine-isoleucine-arginine amide sufficiently inhibits mushroom tyrosinase activity, shows potent antioxidant activity and effectively impedes melanogenesis in cultured melanocytes via cooperative biological activities. Our findings demonstrate the potential utility of the bioactive tetrapeptide from milk proteins as a chemical alternative to hypopigmenting agents.

scholarly journals A Novel and Effective Method for Human Primary Skin Melanocytes and Metastatic Melanoma Cell Isolation

Cancers ◽  
2021 ◽  
Vol 13 (24) ◽  
pp. 6244
Author(s):  
Aneta Ścieżyńska ◽  
Anna Sobiepanek ◽  
Patrycja D. Kowalska ◽  
Marta Soszyńska ◽  
Krzysztof Łuszczyński ◽  
...  
Keyword(s):  
Lymph Node ◽  
Metastatic Melanoma ◽  
Melanoma Cell ◽  
Skin Diseases ◽  
Skin Pigmentation ◽  
Cell Isolation ◽  
Isolated Cells ◽  
Melanin Production ◽  
Metastatic Melanoma Cell ◽  
Pigmentation Disorders

The development of an effective method of melanocyte isolation and culture is necessary for basic and clinical studies concerning skin diseases, including skin pigmentation disorders and melanoma. In this paper, we describe a novel, non-enzymatic and effective method of skin melanocyte and metastatic melanoma cell isolation and culture (along with the spontaneous spheroid creation) from skin or lymph node explants. The method is based on the selective harvesting of melanocytes and melanoma cells emigrating from the cultured explants. Thereby, isolated cells retain their natural phenotypical features, such as expression of tyrosinase and Melan-A as well as melanin production and are not contaminated by keratinocytes and fibroblasts. Such melanocyte and melanoma cell cultures may be very useful for medical and cosmetology studies, including studies of antitumor therapies.

Synthesis of Human Skin Pigmentation Disorders

2016 ◽  
Vol 36 (1) ◽  
pp. 330-344 ◽  
Author(s):  
R. S. Barros ◽  
M. Walter
Keyword(s):  
Human Skin ◽  
Skin Pigmentation ◽  
Pigmentation Disorders

scholarly journals Development of Pigmentation-Regulating Agents by Drug Repositioning

2021 ◽  
Vol 22 (8) ◽  
pp. 3894
Author(s):  
Seo-Mi-Gon Jeong ◽  
Tae-Jin Yoon
Keyword(s):  
Signaling Pathways ◽  
Skin Color ◽  
Research Team ◽  
Drug Repositioning ◽  
Skin Pigmentation ◽  
Melanin Synthesis ◽  
Modern Technique ◽  
Potential Therapeutics ◽  
Distribution Problems ◽  
Pigmentation Disorders

Skin color is determined by the processes of melanin synthesis and distribution. Problems in various molecules or signaling pathways involved in melanin synthesis contribute to skin pigmentation defects. Several trials have been conducted on the production of pigmentation-regulating agents, and drug repositioning has emerged as a modern technique to identify new uses for existing drugs. Our research team has researched substances or drugs associated with pigmentation control and, as a result, nilotinib, sorafenib, and ICG-001 have been found to promote pigmentation, while 5-iodotubercidin inhibits pigmentation. Therefore, these substances or medications were suggested as potential therapeutics for pigmentation disorders by drug repositioning.

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