Physiological and gene expression studies of selected Zea mays L. and Pennisetum glaucum (L.) R. Br. genotypes to simulated drought stress condition

2019 ◽  
Vol 32 (3) ◽  
pp. 397-406 ◽  
Author(s):  
Emmanuel Iwuala ◽  
Victor Odjegba ◽  
Caroline Umebese ◽  
Vinay Sharma ◽  
Afroz Alam
Keyword(s):  
Gene Expression ◽  
Zea Mays ◽  
Drought Stress ◽  
Stress Condition ◽  
Zea Mays L ◽  
Pennisetum Glaucum ◽  
Expression Studies ◽  
Drought Stress Condition ◽  
Simulated Drought ◽  
Gene Expression Studies

scholarly journals Selection of various synthetic Maize (Zea mays L.) genotypes on drought stress condition

2019 ◽  
Vol 235 ◽  
pp. 012027
Author(s):  
M Farid ◽  
Y Musa ◽  
Nasaruddin ◽  
I Ridwan
Keyword(s):  
Zea Mays ◽  
Drought Stress ◽  
Stress Condition ◽  
Zea Mays L ◽  
Drought Stress Condition ◽  
Selection Of

Effect of drought stress on the expression of genes linked to antioxidant enzymatic activity in landraces of Zea mays L. and Pennisetum glaucum (L.) R. Br.

2019 ◽  
Vol 24 (3) ◽  
pp. 422-433 ◽  
Author(s):  
Emmanuel Iwuala ◽  
Victor Odjegba ◽  
Abiodun Ajiboye ◽  
Caroline Umebese ◽  
Vinay Sharma ◽  
...  
Keyword(s):  
Zea Mays ◽  
Drought Stress ◽  
Enzymatic Activity ◽  
Zea Mays L ◽  
Pennisetum Glaucum ◽  
Expression Of Genes ◽  
Antioxidant Enzymatic Activity

scholarly journals Effects of Coronatine on Growth, Gas Exchange Traits, Chlorophyll Content, Antioxidant Enzymes and Lipid Peroxidation in Maize (Zea mays L.) Seedlings under Simulated Drought Stress

2008 ◽  
Vol 11 (3) ◽  
pp. 283-290 ◽  
Author(s):  
Baoqing Wang ◽  
Zhaohu Li ◽  
Egrinya Eneji ◽  
Xiaoli Tian ◽  
Zhixi Zhai ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Zea Mays ◽  
Antioxidant Enzymes ◽  
Drought Stress ◽  
Gas Exchange ◽  
Chlorophyll Content ◽  
Zea Mays L ◽  
Simulated Drought

scholarly journals Cloning and validation of reference genes for normalization of gene expression studies in pearl millet [Pennisetum glaucum (L.) R. Br.] by quantitative real-time PCR

Plant Gene ◽  
2015 ◽  
Vol 1 ◽  
pp. 35-42 ◽  
Author(s):  
Palakolanu Sudhakar Reddy ◽  
Dumbala Srinivas Reddy ◽  
Kiran K. Sharma ◽  
Pooja Bhatnagar-Mathur ◽  
Vincent Vadez
Keyword(s):  
Gene Expression ◽  
Real Time ◽  
Pearl Millet ◽  
Real Time Pcr ◽  
Reference Genes ◽  
Pennisetum Glaucum ◽  
Quantitative Real Time Pcr ◽  
Expression Studies ◽  
Gene Expression Studies

scholarly journals Evaluation and Validation of Housekeeping Genes as Reference for Gene Expression Studies in Pigeonpea (Cajanus cajan) Under Drought Stress Conditions

PLoS ONE ◽  
2015 ◽  
Vol 10 (4) ◽  
pp. e0122847 ◽  
Author(s):  
Pallavi Sinha ◽  
Vikas K. Singh ◽  
V. Suryanarayana ◽  
L. Krishnamurthy ◽  
Rachit K. Saxena ◽  
...  
Keyword(s):  
Gene Expression ◽  
Drought Stress ◽  
Cajanus Cajan ◽  
Housekeeping Genes ◽  
Stress Conditions ◽  
Expression Studies ◽  
Gene Expression Studies

scholarly journals Validation of Reference Genes for Gene Expression Studies in Virus-Infected Nicotiana benthamiana Using Quantitative Real-Time PCR

PLoS ONE ◽  
2012 ◽  
Vol 7 (9) ◽  
pp. e46451 ◽  
Author(s):  
Deshui Liu ◽  
Lindan Shi ◽  
Chenggui Han ◽  
Jialin Yu ◽  
Dawei Li ◽  
...  
Keyword(s):  
Gene Expression ◽  
Real Time ◽  
Real Time Pcr ◽  
Nicotiana Benthamiana ◽  
Reference Genes ◽  
Quantitative Real Time Pcr ◽  
Expression Studies ◽  
Gene Expression Studies

scholarly journals Assessment of common housekeeping genes as reference for gene expression studies using RT-qPCR in mouse choroid plexus

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Kim Hoa Ho ◽  
Annarita Patrizi
Keyword(s):  
Gene Expression ◽  
Choroid Plexus ◽  
Sensory Deprivation ◽  
Housekeeping Genes ◽  
Housekeeping Gene ◽  
Experimental Conditions ◽  
Brain Repair ◽  
Expression Studies ◽  
Testing Algorithms ◽  
Gene Expression Studies

AbstractChoroid plexus (ChP), a vascularized secretory epithelium located in all brain ventricles, plays critical roles in development, homeostasis and brain repair. Reverse transcription quantitative real-time PCR (RT-qPCR) is a popular and useful technique for measuring gene expression changes and also widely used in ChP studies. However, the reliability of RT-qPCR data is strongly dependent on the choice of reference genes, which are supposed to be stable across all samples. In this study, we validated the expression of 12 well established housekeeping genes in ChP in 2 independent experimental paradigms by using popular stability testing algorithms: BestKeeper, DeltaCq, geNorm and NormFinder. Rer1 and Rpl13a were identified as the most stable genes throughout mouse ChP development, while Hprt1 and Rpl27 were the most stable genes across conditions in a mouse sensory deprivation experiment. In addition, Rpl13a, Rpl27 and Tbp were mutually among the top five most stable genes in both experiments. Normalisation of Ttr and Otx2 expression levels using different housekeeping gene combinations demonstrated the profound effect of reference gene choice on target gene expression. Our study emphasized the importance of validating and selecting stable housekeeping genes under specific experimental conditions.

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