Impact of native Wolbachia on reproductive fitness and bacterial pathogens in Aedes aegypti mosquitoes

2021 ◽  
Author(s):  
Sivaraman Balaji ◽  
Solai Ramatchandirane Prabagaran
Keyword(s):  
Aedes Aegypti ◽  
Bacterial Pathogens ◽  
Reproductive Fitness

scholarly journals Plant nutrient quality impacts survival and reproductive fitness of the dengue vector Aedes aegypti

2020 ◽  
Author(s):  
Vincent Odhiambo Nyasembe ◽  
David Poumo Tchouassi ◽  
Martha Njeri Muturi ◽  
Christian W. W. Pirk ◽  
Catherine L. Sole ◽  
...  
Keyword(s):  
Amino Acid ◽  
Aedes Aegypti ◽  
Disease Transmission ◽  
Acid Content ◽  
Amino Acid Content ◽  
Reproductive Fitness ◽  
The Other ◽  
Gut Contents ◽  
Dengue Vector

Abstract Background: In a recent study using DNA barcoding, we identified the plants fed upon by four Afro-tropical mosquito species that vector dengue, malaria, and Rift Valley fever. Herein, we have expanded on this study by investigating the role of three of the plants Pithecellobium dulce (Fabaceae), Leonotis nepetifolia (Lamiaceae), and Opuntia ficus-indica (Cactaceae) on survival, fecundity, and egg viability of the dengue vector Aedes aegypti. Methods: We tested these effects using females that received a) an initial three rations of bloodmeal, and b) received no bloodmeal at all. Two controls were included; age-matched females fed on glucose solution with or without initial bloodmeal, and those fed exclusively on bloodmeal. Data was collected daily over a 30-day period. The amino acid content of Ae. aegypti guts and the amino acid content of their respective diets was detected by coupled liquid chromatography – mass spectrometry.Results: Females fed on P. dulce and exclusive bloodmeal had a shorter survival than those fed on glucose. On the other hand, females fed on L. nepetifolia survived longer than those fed exclusively on bloodmeal, whereas those fed on O. ficus-indica had the shortest survival time. With initial bloodmeal, females fed on L. nepetifolia laid 1.6-fold more eggs while those fed on the other diets laid fewer eggs, compared to those fed exclusively on bloodmeal. Hatching rates of the eggs laid varied with the diet. Mass spectroscopic analysis of gut contents of mosquitoes exposed to the different diets showed qualitative and quantitative differences in their amino acid levels. Conclusion: Our findings highlight the central role of plant nutrients in the reproductive fitness of dengue vectors which may impact their disease transmission potential.

scholarly journals Plant Nutrient Quality Promotes Survival and Reproductive Fitness of the Dengue Vector Aedes Aegypti

2020 ◽  
Author(s):  
Vincent Odhiambo Nyasembe ◽  
David P. Tchouassi ◽  
Martha N. Muturi ◽  
Christian W. W. Pirk ◽  
Catherine L. Sole ◽  
...  
Keyword(s):  
Amino Acid ◽  
Aedes Aegypti ◽  
Disease Transmission ◽  
Acid Content ◽  
Amino Acid Content ◽  
Reproductive Fitness ◽  
The Other ◽  
Gut Contents ◽  
Dengue Vector

Abstract Background: In a recent study using DNA barcoding, we identified the plants fed upon by four Afro-tropical mosquito species that vector dengue, malaria, and Rift Valley fever. Herein, we have expanded on this study by investigating the role of three of the plants Pithecellobium dulce (Fabaceae), Leonotis nepetifolia (Lamiaceae), and Opuntia ficus-indica (Cactaceae) on survival, fecundity, and egg viability of the dengue vector Aedes aegypti. Methods: We tested these effects using females that received a) an initial three rations of bloodmeal, and b) received no bloodmeal at all. Two controls were included; age-matched females fed on glucose solution with or without initial bloodmeal, and those fed exclusively on bloodmeal. Data was collected daily over a 30-day period. The amino acid content of Ae. aegypti guts and the amino acid content of their respective diets was detected by coupled liquid chromatography – mass spectrometry.Results: Females fed on P. dulce and exclusive bloodmeal had a shorter survival than those fed on glucose. On the other hand, females fed on L. nepetifolia survived longer than those fed exclusively on bloodmeal, whereas those fed on O. ficus-indica had the shortest survival time. With initial bloodmeal, females fed on L. nepetifolia laid 1.6-fold more eggs while those fed on the other diets laid fewer eggs, compared to those fed exclusively on bloodmeal. Hatching rates of the eggs laid varied with the diet. Mass spectroscopic analysis of gut contents of mosquitoes exposed to the different diets showed qualitative and quantitative differences in their amino acid levels. Conclusion: Our findings highlight the central role of plant nutrients in the reproductive fitness of dengue vectors which may impact their disease transmission potential.

scholarly journals Plant nutrient quality promotes survival and reproductive fitness of the dengue vector Aedes aegypti

2020 ◽  
Author(s):  
Vincent Odhiambo Nyasembe ◽  
David P. Tchouassi ◽  
Martha N. Muturi ◽  
Christian W. W. Pirk ◽  
Catherine L. Sole ◽  
...  
Keyword(s):  
Amino Acid ◽  
Aedes Aegypti ◽  
Disease Transmission ◽  
Acid Content ◽  
Amino Acid Content ◽  
Reproductive Fitness ◽  
The Other ◽  
Gut Contents ◽  
Dengue Vector

Abstract Background In a recent study using DNA barcoding, we identified the plants fed upon by four Afro-tropical mosquito species that vector dengue, malaria, and Rift Valley fever. Herein, we have expanded on this study by investigating the role of three of the plants Pithecellobium dulce (Fabaceae), Leonotis nepetifolia (Lamiaceae), and Opuntia ficus-indica (Cactaceae) on survival, fecundity, and egg viability of the dengue vector Aedes aegypti. Methods We tested these effects using females that received a) an initial three rations of bloodmeal, and b) received no bloodmeal at all. Two controls were included; age-matched females fed on glucose solution with or without initial bloodmeal, and those fed exclusively on bloodmeal. Data was collected daily over a 30-day period. The amino acid content of Ae. aegypti guts and the amino acid content of their respective diets was detected by coupled liquid chromatography – mass spectrometry. Results Females fed on P. dulce and exclusive bloodmeal had a shorter survival than those fed on glucose. On the other hand, females fed on L. nepetifolia survived longer than those fed exclusively on bloodmeal, whereas those fed on O. ficus-indica had the shortest survival time. With initial bloodmeal, females fed on L. nepetifolia laid 1.6-fold more eggs while those fed on the other diets laid fewer eggs, compared to those fed exclusively on bloodmeal. Hatching rates of the eggs laid varied with the diet. Mass spectroscopic analysis of gut contents of mosquitoes exposed to the different diets showed qualitative and quantitative differences in their amino acid levels. Conclusion Our findings highlight the central role of plant nutrients in the reproductive fitness of dengue vectors which may impact their disease transmission potential.

scholarly journals Impact of the Argemone mexicana Stem Extracts on the Reproductive Fitness and Behavior of Adult Dengue Vector, Aedes aegypti L. (Diptera: Culicidae)

2014 ◽  
Vol 6 ◽  
pp. IJIS.S19006
Author(s):  
Radhika Warikoo ◽  
Sarita Kumar
Keyword(s):  
Aedes Aegypti ◽  
Petroleum Ether ◽  
Reproductive Fitness ◽  
Oviposition Deterrent ◽  
Dengue Vector ◽  
Argemone Mexicana ◽  
Egg Hatch ◽  
And Behavior ◽  
The Impact ◽  
Benzene Extract

Present investigations evaluated the impact of Argemone mexicana stem extracts on the reproductive fitness of dengue vector, Aedes aegypti, assessed in terms of oviposition deterrent and ovicidal potential. The oviposition deterrent studies of the extracts, prepared using petroleum ether, hexane, benzene, acetone, and ethanol as the solvents, revealed the maximum deterrence potency of the petroleum ether extracts with a significant 15.6% ED at 60 ppm rising by 83.8% at 1000 ppm to 99.4% effective deterrence (ED). Other stem extracts were found to be ineffective at 60 ppm, though resulted in 85.3–96.2% ED and diminished fecundity in A. aegypti at 1000 ppm. Further, A. mexicana stem extracts exhibited moderate ovicidal potential against A. aegypti eggs causing only 42.65–67.85% egg mortality at 1000 ppm, the lowest hatch of 32.15% caused by the benzene extract. Other stem extracts also failed to express effective ovicidal potency with the percent egg hatch ranging between 96.6 and 99.0 at 400 ppm, and 78.8 and 99.0 at 600 ppm. Our results suggest the significant but variable efficacy of A. mexicana stem extracts causing reproductive disadvantage in A. aegypti.

scholarly journals Physiological and reproductive fitness cost in Aedes aegypti on exposure to toxic xenobiotics in New Delhi, India

2021 ◽  
Vol 13 (1) ◽  
pp. 71-78
Author(s):  
Addea Gupta ◽  
Roopa Rani Samal ◽  
Sarita Kumar
Keyword(s):  
Aedes Aegypti ◽  
Negative Impact ◽  
Reproductive Fitness ◽  
Fourth Instar ◽  
Adult Longevity ◽  
Reproduction Rate ◽  
Net Reproductive Rate ◽  
Susceptible Population ◽  
Exposed Population ◽  
The Impact

Aedes aegypti, is a well-known vector of dengue, Chikungunya and Zika at the global level. Primary use of pyrethroids as control interventions has caused the development of a considerable level of immunity in Ae. aegypti. The current study assessed the efficacy of a pyrethroid, ?-cypermethrin on the survival and various life parameters of Ae. aegypti. The larvicidal studies with ?-cypermethrin revealed the respective LC50 and LC90 values as 0.26526 mg/L and 0.60211 mg/L. The impact of LC50 level was assessed on the growth and life attributes; such as gonotrophic cycle, egg development, hatchability, development and survival of immature stages, adult longevity, reproduction rate and generation time; of fourth instar of susceptible (S) and ?-cypermethrin-exposed population (E). The exposed population showed diminished fitness as compared to the susceptible population. The individual female fecundity in susceptible population was recorded as 79.6 with 61.6% hatchability rate as compared to the 28 eggs/female and 25% hatchability in the exposed population. The mean egg hatch time in S strain increased by 2-fold in E strain. The proportion of immature survival observed in S strain was 0.88 for fourth instar to pupa (P/I), 0.94 for pupa to adult (A/P) and an overall 0.83 for fourth larva to adult (A/I), which respectively reduced to 0.32, 0.86 and 0.27 in E strain of Ae. aegypti. Likewise, the net reproductive rate, birth rate and death rate were significantly (p < 0.05) higher in S than in E strain. This study demonstrates the negative impact of ?-cypermethrin on the physiological and reproductive fitness of Ae. aegypti.

scholarly journals Aedes aegypti Infection With Trypanosomatid Strigomonas culicis Alters Midgut Redox Metabolism and Reduces Mosquito Reproductive Fitness

2021 ◽  
Vol 11 ◽  
Author(s):  
Ana Cristina S. Bombaça ◽  
Ana Caroline P. Gandara ◽  
Vitor Ennes-Vidal ◽  
Vanessa Bottino-Rojas ◽  
Felipe A. Dias ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Aedes Aegypti ◽  
Antioxidant System ◽  
Control Strategies ◽  
Vector Competence ◽  
Health Impact ◽  
Reproductive Fitness ◽  
Insect Immunity ◽  
Transcriptional Modulation ◽  
Immune Related Genes

Aedes aegypti mosquitoes transmit arboviruses of important global health impact, and their intestinal microbiota can influence vector competence by stimulating the innate immune system. Midgut epithelial cells also produce toxic reactive oxygen species (ROS) by dual oxidases (DUOXs) that are essential players in insect immunity. Strigomonas culicis is a monoxenous trypanosomatid that naturally inhabits mosquitoes; it hosts an endosymbiotic bacterium that completes essential biosynthetic pathways of the parasite and influences its oxidative metabolism. Our group previously showed that S. culicis hydrogen peroxide (H2O2)-resistant (WTR) strain is more infectious to A. aegypti mosquitoes than the wild-type (WT) strain. Here, we investigated the influence of both strains on the midgut oxidative environment and the effect of infection on mosquito fitness and immunity. WT stimulated the production of superoxide by mitochondrial metabolism of midgut epithelial cells after 4 days post-infection, while WTR exacerbated H2O2 production mediated by increased DUOX activity and impairment of antioxidant system. The infection with both strains also disrupted the fecundity and fertility of the females, with a greater impact on reproductive fitness of WTR-infected mosquitoes. The presence of these parasites induced specific transcriptional modulation of immune-related genes, such as attacin and defensin A during WTR infection (11.8- and 6.4-fold, respectively) and defensin C in WT infection (7.1-fold). Thus, we propose that A. aegypti oxidative response starts in early infection time and does not affect the survival of the H2O2-resistant strain, which has a more efficient antioxidant system. Our data provide new biological aspects of A. aegypti–S. culicis relationship that can be used later in alternative vector control strategies.

Intrinsic studies of Euphorbia antiquorum L . latex extracts against human bacterial pathogens and mosquito vector Aedes aegypti , Culex quinquefasciatus (Diptera: Culicidae)

2017 ◽  
Vol 10 ◽  
pp. 75-82 ◽  
Author(s):  
Rajkuberan Chandrasekaran ◽  
Sathishkumar Gnanasekar ◽  
Prabukumar Seetharaman ◽  
Muthukumar Krishnan ◽  
Sivaramakrishnan Sivaperumal
Keyword(s):  
Aedes Aegypti ◽  
Culex Quinquefasciatus ◽  
Bacterial Pathogens ◽  
Mosquito Vector

scholarly journals Plant nutrient quality impacts survival and reproductive fitness of the dengue vector Aedes aegypti

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Vincent Odhiambo Nyasembe ◽  
David Poumo Tchouassi ◽  
Martha Njeri Muturi ◽  
Christian W. W. Pirk ◽  
Catherine L. Sole ◽  
...  
Keyword(s):  
Amino Acid ◽  
Aedes Aegypti ◽  
Blood Meal ◽  
Reproductive Fitness ◽  
The Other ◽  
Gut Contents ◽  
Dengue Vector ◽  
Initial Blood ◽  
Blood Meals

Abstract Background In a recent study using DNA barcoding, we identified the plants fed upon by four Afro-tropical mosquito species that vector dengue, malaria, and Rift Valley fever. Herein, we have expanded on this study by investigating the role of three of the plants, Pithecellobium dulce (Fabaceae), Leonotis nepetifolia (Lamiaceae), and Opuntia ficus-indica (Cactaceae), on the survival, fecundity, and egg viability of the dengue vector Aedes aegypti. Methods We tested these effects using females that received (i) an initial three rations of blood meals and (ii) no blood meal at all. Two controls were included: age-matched females fed on glucose solution with or without an initial blood meal and those fed exclusively on blood meals. Data were collected daily over a 30-day period. The amino acid contents of Ae. aegypti guts and their respective diets were detected by coupled liquid chromatography-mass spectrometry. Results Females fed on P. dulce and an exclusively blood meal diet had a shorter survival than those fed on glucose. On the other hand, females fed on L. nepetifolia survived longer than those fed exclusively on blood meals, whereas those fed on O. ficus-indica had the shortest survival time. With an initial blood meal, females fed on L. nepetifolia laid 1.6-fold more eggs while those fed on the other diets laid fewer eggs compared to those fed exclusively on blood meals. Hatching rates of the eggs laid varied with the diet. Mass spectroscopic analysis of gut contents of mosquitoes exposed to the different diets showed qualitative and quantitative differences in their amino acid levels. Conclusion Our findings highlight the central role of plant nutrients in the reproductive fitness of dengue vectors, which may impact their disease transmission potential.

Sign in / Sign up

Export Citation Format

Share Document