scholarly journals Characterization of multidrug-resistant avian pathogenic Escherichia coli: an outbreak in canaries

2021 ◽  
Author(s):  
Angela Hitomi Kimura ◽  
Vanessa Lumi Koga ◽  
Luís Eduardo de Souza Gazal ◽  
Benito Guimarães de Brito ◽  
Kelly Cristina Tagliari de Brito ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Multidrug Resistant ◽  
Avian Pathogenic Escherichia Coli ◽  
Pathogenic Escherichia Coli

scholarly journals Complete Genomic and Lysis-Cassette Characterization of the Novel Phage, KBNP1315, which Infects Avian Pathogenic Escherichia coli (APEC)

PLoS ONE ◽  
2015 ◽  
Vol 10 (11) ◽  
pp. e0142504 ◽  
Author(s):  
Jung Seok Lee ◽  
Ho Bin Jang ◽  
Ki Sei Kim ◽  
Tae Hwan Kim ◽  
Se Pyeong Im ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
The Novel ◽  
Avian Pathogenic Escherichia Coli ◽  
Pathogenic Escherichia Coli ◽  
Lysis Cassette ◽  
Complete Genomic

scholarly journals Characterization of Avian Pathogenic Escherichia coli (APEC) Associated With Turkey Cellulitis in Iowa

2020 ◽  
Vol 7 ◽  
Author(s):  
Aline Luisa de Oliveira ◽  
Darby M. Newman ◽  
Yuko Sato ◽  
Andrew Noel ◽  
Britney Rauk ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Avian Pathogenic Escherichia Coli ◽  
Pathogenic Escherichia Coli

scholarly journals Multidrug-Resistant Avian Pathogenic Escherichia coli Strains and Association of Their Virulence Genes in Bangladesh

2020 ◽  
Vol 8 (8) ◽  
pp. 1135
Author(s):  
Otun Saha ◽  
M. Nazmul Hoque ◽  
Ovinu Kibria Islam ◽  
Md. Mizanur Rahaman ◽  
Munawar Sultana ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Virulence Genes ◽  
Multidrug Resistant ◽  
Avian Pathogenic Escherichia Coli ◽  
E Coli ◽  
Molecular Approaches ◽  
Clade B ◽  
Pathogenic Escherichia Coli ◽  
Resistance Patterns

The avian pathogenic Escherichia coli (APEC) strains are the chief etiology of colibacillosis worldwide. The present study investigated the circulating phylotypes, existence of virulence genes (VGs), and antimicrobial resistance (AMR) in 392 APEC isolates, obtained from 130 samples belonged to six farms using both phenotypic and PCR-based molecular approaches. Congo red binding (CRB) assay confirmed 174 APEC isolates which were segregated into ten, nine, and eight distinct genotypes by RAPD assay (discriminatory index, DI = 0.8707), BOX-PCR (DI = 0.8591) and ERIC-PCR (DI = 0.8371), respectively. The combination of three phylogenetic markers (chuA, yjaA and DNA fragment TspE4.C2) classified APEC isolates into B23 (37.36%), A1 (33.91%), D2 (11.49%), B22 (9.20%), and B1 (8.05%) phylotypes. Majority of the APEC isolates (75–100%) harbored VGs (ial, fimH, crl, papC, and cjrC). These VGs (papC and cjrC) and phylotypes (D2 and B2) of APEC had significant (p = 0.004) association with colibacillosis. Phylogenetic analysis showed two distinct clades (clade A and clade B) of APEC, where clade A had 98–100% similarity with E. coli APEC O78 and E. coli EHEC strains, and clade B had closest relationship with E. coli O169:H41 strain. Interestingly, phylogroups B2 and D2 were found in the APEC strains of both clades, while the strains from phylogroups A1 and B1 were found in clade A only. In this study, 81.71% of the isolates were biofilm formers, and possessed plasmids of varying ranges (1.0 to 54 kb). In vitro antibiogram profiling revealed that 100% isolates were resistant to ≥3 antibiotics, of which 61.96%, 55.24%, 53.85%, 51.16% and 45.58% isolates in phylotypes B1, D2, B22, B23, and A1, respectively, were resistant to these antimicrobials. The resistance patterns varied among different phylotypes, notably in phylotype B22, showing the highest resistance to ampicillin (90.91%), nalidixic acid (90.11%), tetracycline (83.72%), and nitrofurantoin (65.12%). Correspondence analysis also showed significant correlation among phylotypes with CRB (p = 0.008), biofilm formation (p = 0.02), drug resistance (p = 0.03), and VGs (p = 0.06). This report demonstrated that B2 and A1 phylotypes are dominantly circulating APEC phylotypes in Bangladesh; however, B2 and D2 are strongly associated with the pathogenicity. A high prevalence of antibiotic-resistant APEC strains from different phylotypes suggest the use of organic antimicrobial compounds, and/or metals, and the rotational use of antibiotics in poultry farms in Bangladesh.

scholarly journals CHARACTERIZATION OF A SERIES OF TRANSCONJUGANT MUTANTS OF AN AVIAN PATHOGENIC ESCHERICHIA COLI ISOLATE FOR RESISTANCE TO SERUM COMPLEMENT

2007 ◽  
Vol 2 (3) ◽  
pp. e23-e23
Author(s):  
AARON M. LYNNE ◽  
JEROD A. SKYBERG ◽  
CATHERINE M. LOGUE ◽  
CURT DOETKOTT ◽  
STEVEN L. FOLEY ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Serum Complement ◽  
Avian Pathogenic Escherichia Coli ◽  
Coli Isolate ◽  
Pathogenic Escherichia Coli
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