Chromosome morphology and cytomolecular characteristics of the perennial rye cultivar ‘Kriszta’

AbstractThe perennial Secale cereanum cultivar ‘Kriszta’ is an artificial hybrid of S. cereale and S. strictum ssp. anatolicum. From the cross between the wheat line Mv9kr1 and ‘Kriszta’, which aimed the transfer of beneficial traits from rye to wheat, numerous translocation lines have been produced. For the identification of the translocated chromosomes, the unambiguous differentiation between chromosome arms of ‘Kriszta’ is essential. The identification of its short chromosome arms using conventional FISH probes is easy, but because of their similar hybridization patterns, its long arms cannot be distinguished. The present study aimed to create the detailed karyotype of ‘Kriszta’, especially that of long arms, by both chromosome measurements and FISH using highly repetitive, as well as subtelomeric tandem repeat, and synthetic microsatellite DNA sequences. Our results indicate that the chromosome complement of ‘Kriszta’ is not a simple combination of the chromosomes of the parental rye species but is composed of rearranged chromosomes. It is also showed that an adequate pair-wise combination of the DNA sequences pSc119.2, pSc200, pSc250, and (AAC)5 makes it possible to identify any of the long arms of S. cereanum cv. Kriszta chromosomes. The future usability of the identified wheat- ‘Kriszta’ translocated chromosomes is also discussed.

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Polymorphism of microsatellite DNA sequences in dogs Russian toy terrier and German dog

The Animal Biology ◽

10.15407/animbiol18.01.027 ◽

2016 ◽

Vol 18 (1) ◽

pp. 27-32 ◽

Cited By ~ 1

Author(s):

V. Dzitsiuk ◽

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S. Kruhlyk ◽

V. Spyrydonov ◽

◽

...

Keyword(s):

Dna Sequences ◽

Microsatellite Dna

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Chromosome morphology of Clematis, subsection Viornae (Ranunculaceae)

Canadian Journal of Botany ◽

10.1139/b76-122 ◽

1976 ◽

Vol 54 (10) ◽

pp. 1135-1139 ◽

Cited By ~ 5

Author(s):

W. Michael Dennis

Keyword(s):

Chromosome Number ◽

Somatic Chromosome ◽

Chromosome Complement ◽

Chromosome Morphology ◽

Terminal Region ◽

Somatic Chromosome Number ◽

Median Region ◽

Acrocentric Chromosomes ◽

Subterminal Region ◽

Marked Stability

Cytological studies were made on the following taxa: C. addisonii, C. filifera, C. glaucophylla, C. pitcheri, C. reticulata, C. texensis, C. versicolor, and C. viorna. All species were found to have a somatic chromosome number of 16 with a uniform karyotype consisting of five pairs of metacentric chromosomes with centromeres in the median region and three pairs of acrocentric chromosomes, two pairs with centromeres in the terminal region and one pair with centromeres in the subterminal region. These findings agree with reports of chromosome number and karyotype for other species of Clematis and suggest a marked stability of chromosome complement in the genus.

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Molecular and cytogenetic analysis of repetitive DNA in pea (Pisum sativum L.)

Genome ◽

10.1139/g01-056 ◽

2001 ◽

Vol 44 (4) ◽

pp. 716-728 ◽

Cited By ~ 21

Author(s):

Pavel Neumann ◽

Marcela Nouzová ◽

Jirí Macas

Keyword(s):

Pisum Sativum ◽

Repetitive Dna ◽

Dna Sequences ◽

Genomic Dna ◽

Tandem Repeats ◽

Genomic Library ◽

Chromosome Morphology ◽

Plant Genome ◽

Genomic Repeats ◽

Dispersed Repeats

A set of pea DNA sequences representing the most abundant genomic repeats was obtained by combining several approaches. Dispersed repeats were isolated by screening a short-insert genomic library using genomic DNA as a probe. Thirty-two clones ranging from 149 to 2961 bp in size and from 1000 to 39 000/1C in their copy number were sequenced and further characterized. Fourteen clones were identified as retrotransposon-like sequences, based on their homologies to known elements. Fluorescence in situ hybridization using clones of reverse transcriptase and integrase coding sequences as probes revealed that corresponding retroelements were scattered along all pea chromosomes. Two novel families of tandem repeats, named PisTR-A and PisTR-B, were isolated by screening a genomic DNA library with Cot-1 DNA and by employing genomic self-priming PCR, respectively. PisTR-A repeats are 211212 bp long, their abundance is 2 × 104 copies/1C, and they are partially clustered in a secondary constriction of one chromosome pair with the rest of their copies dispersed on all chromosomes. PisTR-B sequences are of similar abundance (104 copies/1C) but differ from the "A" family in their monomer length (50 bp), high A/T content, and chromosomal localization in a limited number of discrete bands. These bands are located mainly in (sub)telomeric and pericentromeric regions, and their patterns, together with chromosome morphology, allow discrimination of all chromosome types within the pea karyotype. Whereas both tandem repeat families are mostly specific to the genus Pisum, many of the dispersed repeats were detected in other legume species, mainly those in the genus Vicia.Key words: repetitive DNA, plant genome, retroelements, satellite DNA, Pisum sativum.

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Redescription of the karyotype of five species of the family Bufonidae (Amphibia: Anura) from central area of Argentina

Biologia ◽

10.2478/s11756-011-0048-8 ◽

2011 ◽

Vol 66 (3) ◽

Cited By ~ 3

Author(s):

Mariana Baraquet ◽

Julián Valetti ◽

Nancy Salas ◽

Adolfo Martino

Keyword(s):

Significant Contribution ◽

Average Length ◽

Chromosome Complement ◽

Central Area ◽

Chromosome Length ◽

Chromosome Morphology ◽

Morphometric Parameters ◽

Classification Rate ◽

The Family ◽

Karyotype Formula

AbstractIn this study karyotypic features of the five species of the family Bufonidae from the central area of Argentina are described. The species are Rhinella achalensis, Rhinella arenarum, Rhinella fernandezae, Rhinella schneideri and Melanophryniscus stelzneri. The metaphases were obtained from intestinal and testis cells, using conventional techniques. Twenty metaphasic figures per individual were analyzed and the total length of each chromosome and the length of the four arms were measured. The obtained measurements were processed using Excel 2000 to obtain the average length of the arms p and q, the arm ratio, the centromeric index, the relative chromosome length and the relative arm length. All species showed karyotype 2n = 22, and karyotype formula of 6: 5. Pairs one to six were large, with a relative chromosome length between 18.64–7.59%; pairs seven to eleven were small, with a relative chromosome length between 7.18–2.42%. In all species the chromosome morphology was metacentric or submetacentric. Karyotype and ideograms were made for all species, based on morphometric parameters of the chromosome complement. Finally, discriminant analysis was used to separate the five species analyzed, with a highly significant classification rate of 80% and P < 0.0001. These results agree, in general, with those presented by other authors, however, in M. stelzneri detailed karyological studied have not been made so far, thus this work represents a significant contribution to the karyotypic decryption features of this species and the Rhinellla species from central area of Argentina.

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Identification of the entire chromosome complement of bread wheat by two-colour FISH

Genome ◽

10.1139/g97-077 ◽

1997 ◽

Vol 40 (5) ◽

pp. 589-593 ◽

Cited By ~ 125

Author(s):

C. Pedersen ◽

P. Langridge

Keyword(s):

In Situ Hybridization ◽

Fluorescence In Situ Hybridization ◽

Dna Sequences ◽

Banding Pattern ◽

Aegilops Tauschii ◽

Chromosome Complement ◽

Chromosome Identification ◽

Satellite Sequence ◽

Entire Chromosome

Using the Aegilops tauschii clone pAs1 together with the barley clone pHvG38 for two-colour fluorescence in situ hybridization (FISH) the entire chromosome complement of hexaploid wheat was identified. The combination of the two probes allowed easy discrimination of the three genomes of wheat. The banding pattern obtained with the pHvG38 probe containing the GAA-satellite sequence was identical to the N-banding pattern of wheat. A detailed idiogram was constructed, including 73 GAA bands and 48 pAs1 bands. Identification of the wheat chromosomes by FISH will be particularly useful in connection with the physical mapping of other DNA sequences to chromosomes, or for chromosome identification in general, as an alternative to C-banding.Key words: Triticum aestivum, chromosome identification, fluorescence in situ hybridization, repetitive DNA sequences.

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Binding of mismatched microsatellite DNA sequences by the human MSH2 protein

Science ◽

10.1126/science.7973733 ◽

1994 ◽

Vol 266 (5189) ◽

pp. 1403-1405 ◽

Cited By ~ 110

Author(s):

R Fishel ◽

A Ewel ◽

S Lee ◽

M. Lescoe ◽

J Griffith

Keyword(s):

Dna Sequences ◽

Microsatellite Dna ◽

Msh2 Protein

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Abundance and variation of microsatellite DNA sequences in beans (Phaseolus andVigna)

Genome ◽

10.1139/gen-42-1-27 ◽

1999 ◽

Vol 42 (1) ◽

pp. 27-34 ◽

Cited By ~ 39

Author(s):

Kangfu Yu ◽

Soon J. Park ◽

Vaino Poysa

Keyword(s):

Dna Sequences ◽

Microsatellite Dna

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CYTOGENETIC STUDIES IN ARABIDOPSIS THALIANA

Canadian Journal of Genetics and Cytology ◽

10.1139/g70-032 ◽

1970 ◽

Vol 12 (2) ◽

pp. 217-223 ◽

Cited By ~ 32

Author(s):

Lotti M. S. Sears ◽

Suzanne Lee-Chen

Keyword(s):

Arabidopsis Thaliana ◽

Chromosome Complement ◽

Short Chromosome ◽

Linkage Groups ◽

Primary Trisomics ◽

Cytogenetic Studies

The five primary trisomics and one telotrisomic of Arabidopsis have been established and identified with respect to known linkage groups. From diplotene preparations the chromosome complement was seen to comprise one long, three medium, and one short chromosome. All the trisomics except Fragilis were transmitted through the male (in frequencies up to 22%). There appears to be some selection against disomic eggs, only 21-30% recovery being observed from selfed trisomics. No tetrasomics were found.

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