Prolactin is Expressed in Uterine Leiomyomas and Promotes Signaling and Fibrosis in Myometrial Cells

2021 ◽  
Author(s):  
Alessandra DiMauro ◽  
Christina Seger ◽  
Briaunna Minor ◽  
Andrea M. Amitrano ◽  
Ifunanya Okeke ◽  
...  
Keyword(s):  
Uterine Leiomyomas ◽  
Myometrial Cells

scholarly journals Uterine-Specific Loss of Tsc2 Leads to Myometrial Tumors in Both the Uterus and Lungs

2013 ◽  
Vol 27 (9) ◽  
pp. 1403-1414 ◽  
Author(s):  
Hen Prizant ◽  
Aritro Sen ◽  
Allison Light ◽  
Sung-Nam Cho ◽  
Francesco J. DeMayo ◽  
...  
Keyword(s):  
Lung Transplantation ◽  
Therapeutic Strategies ◽  
Uterine Leiomyomas ◽  
Benign Tumors ◽  
Aromatase Inhibition ◽  
Steroid Production ◽  
Myometrial Cells ◽  
Genes Encoding ◽  
Novel Therapeutic Strategies ◽  
Inactivating Mutations

Lymphangioleiomyomatosis (LAM) is a rare disease characterized by proliferation of abnormal smooth-muscle cells in the lungs, leading to functional loss and sometimes lung transplantation. Although the origin of LAM cells is unknown, several features of LAM provide clues. First, LAM cells contain inactivating mutations in genes encoding Tsc1 or Tsc2, proteins that limit mTORC1 activity. Second, LAM tumors recur after lung transplantation, suggesting a metastatic pathogenesis. Third, LAM is found almost exclusively in women. Finally, LAM shares features with uterine leiomyomas, benign tumors of myometrial cells. From these observations, we proposed that LAM cells might originate from uterine leiomyomas containing Tsc mutations. To test our hypothesis, and to develop mouse models for leiomyoma and LAM, we targeted Tsc2 deletion primarily in uterine cells. In fact, nearly 100% of uteri from uterine-specific Tsc2 knockout mice developed myometrial proliferation and uterine leiomyomas by 12 and 24 weeks, respectively. Myometrial proliferation and mTORC1/S6 activity were abrogated by the mTORC1 inhibitor rapamycin or by elimination of sex steroid production through ovariectomy or aromatase inhibition. In ovariectomized Tsc2 null mice, mTORC1/S6 activity and myometrial growth were restored by estrogen but not progesterone. Thus, even without Tsc2, estrogen appears to be required for myometrial mTORC1/S6 signaling and proliferation. Finally, we found Tsc2 null myometrial tumors in lungs of older Tsc2 uterine-specific knockout females, suggesting that lung LAM-like myometrial lesions may indeed originate from the uterus. This mouse model may improve our understanding of LAM and leiomyomas and might lead to novel therapeutic strategies for both diseases.

scholarly journals Parity associates with chromosomal damage in uterine leiomyomas

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Heli Kuisma ◽  
Simona Bramante ◽  
Kristiina Rajamäki ◽  
Lauri J. Sipilä ◽  
Eevi Kaasinen ◽  
...  
Keyword(s):  
Dna Repair ◽  
Chromosomal Aberrations ◽  
Uterine Leiomyoma ◽  
Uterine Leiomyomas ◽  
Mechanical Forces ◽  
Chromosomal Damage ◽  
Dna Breaks ◽  
Myometrial Cells ◽  
Cellular Environment ◽  
Physiological Forces

AbstractMechanical forces in a constrained cellular environment were recently established as a facilitator of chromosomal damage. Whether this could contribute to tumorigenesis is not known. Uterine leiomyomas are common neoplasms that display relatively few chromosomal aberrations. We hypothesized that if mechanical forces contribute to chromosomal damage, signs of this could be seen in uterine leiomyomas from parous women. We examined the karyotypes of 1946 tumors, and found a striking overrepresentation of chromosomal damage associated with parity. We then subjected myometrial cells to physiological forces similar to those encountered during pregnancy, and found this to cause DNA breaks and a DNA repair response. While mechanical forces acting in constrained cellular environments may thus contribute to neoplastic degeneration, and genesis of uterine leiomyoma, further studies are needed to prove possible causality of the observed association. No evidence for progression to malignancy was found.

scholarly journals Superoxide anion increases intracellular free calcium in human myometrial cells.

1990 ◽  
Vol 265 (36) ◽  
pp. 22533-22536
Author(s):  
N Masumoto ◽  
K Tasaka ◽  
A Miyake ◽  
O Tanizawa
Keyword(s):  
Superoxide Anion ◽  
Intracellular Free Calcium ◽  
Free Calcium ◽  
Myometrial Cells

Uterine leiomyomas revisited with review of literature

2021 ◽  
Author(s):  
Rishi Philip Mathew ◽  
Swati Francis ◽  
Vinayak Jayaram ◽  
Shameema Anvarsadath
Keyword(s):  
Uterine Leiomyomas ◽  
Review Of Literature

scholarly journals Identification of pluripotent cells in bovine uterus: in situ and in vitro studies

Reproduction ◽  
2015 ◽  
Vol 149 (4) ◽  
pp. 317-327 ◽  
Author(s):  
Martyna Łupicka ◽  
Gabriel Bodek ◽  
Nahum Shpigel ◽  
Ehud Elnekave ◽  
Anna J Korzekwa
Keyword(s):  
Flow Cytometry ◽  
Protein Expression ◽  
Mrna Expression ◽  
Uterine Horn ◽  
Uterine Tissue ◽  
Pluripotent Cells ◽  
Flow Cytometry Assay ◽  
Myometrial Cells ◽  
Gene And Protein Expression

The aim of this study was to identify uterine pluripotent cells both in bovine uterine tissues as well in epithelial, stromal, and myometrial uterine cell populations. Moreover, the relationship of pluripotent markers expression with age and the uterine horn side was considered. Uterine tissue was collected from ipsilateral and contralateral horns (days 8–10 of the estrous cycle). Immunohistostaining for C-KIT, OCT3/4, NANOG, and SOX2 in uterine tissue was determined. mRNA expression of C-KIT, OCT3/4, NANOG and SOX2 was evaluated in uterine tissue relative to the age of the cow and uterine horn side. Gene and protein expression of these markers in the uterine luminal epithelial, stromal, and myometrial cells was evaluated by real-time PCR and western blotting respectively. The expression of pluripotent cell markers OCT3/4, NANOG, and SOX2 was identified by flow cytometry assay in epithelial, stromal, and myometrial cells. Multilineage differentiation of the bovine uterine cells was performed. mRNA expression of OCT3/4, NANOG, and SOX2 in uterine tissue was higher in the ipsilateral horn than in the contralateral horn. Flow cytometry assay revealed positive fluorescence for OCT3/4, NANOG, and SOX2 in all uterine cell types. Results showed the age-dependent expression of pluripotent markers in uterine tissue. Beside, the different expression of pluripotent cells in each horn of uterus suggests the influence of ovarian hormones on these characteristics. The highest mRNA and protein expression for pluripotent markers was observed in stromal cells among uterine cells, which indicates this population of cells as the main site of pluripotent cells in the cow uterus.

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