Monosomic Analysis

2012 ◽  
Keyword(s):  
Monosomic Analysis

Monosomic analysis of leaf peroxidase Px0 and Px9 loci in hexaploid oats

Genome ◽  
1988 ◽  
Vol 30 (2) ◽  
pp. 99-102 ◽  
Author(s):  
Toshinobu Morikawa
Keyword(s):  
Enzymatic Activity ◽  
Peroxidase Activity ◽  
Avena Sativa ◽  
Leaf Blade ◽  
Flag Leaf ◽  
Phenotypic Expression ◽  
Avena Fatua ◽  
Monosomic Analysis ◽  
Peroxidase Isoenzymes

Inheritance of the peroxidase isoenzymes of the flag leaf blade was examined by isoelectrofocusing in the hexaploid oats Avena byzantina cv. Kanota, Avena fatua ssp. compacta, and Avena sativa cv. Cherokee. Two independent peroxidase loci (Px0 and Px9) were detected in the F2 from the 'Kanota' × compacta cross. The Px0a derived from compacta expressed the highest peroxidase activity and was accompanied by a post-transcriptionally modified form or mozyme. A monosomic analysis of the Px0 and Px9 loci revealed that they were located on chromosomes 18 and 6, respectively. Phenotypic expression of the peroxidases varied in each genotye at the Px0 and Px9 loci. Phenotypes of the homozygote (Px0aPx0a) and the hemizygote (Px0a—) were similar to each other. The heterozygote (Px0aPx0b) had half the enzymatic activity of the others. Px9b of compacta was functional as a suppressor but that of 'Cherokee' was nonfunctional.Key words: monosomic analysis, peroxidase loci, isoenzyme, hexaploid oats.

scholarly journals MONOSOMIC ANALYSIS OF A CHLOROSIS GENE Ch1 IN COMMON WHEAT

1975 ◽  
Vol 50 (2) ◽  
pp. 151-154 ◽  
Author(s):  
KOICHIRO TSUNEWAKI
Keyword(s):  
Common Wheat ◽  
Monosomic Analysis

Complementary genes for reaction to Puccinia recondita tritici in Triticum aestivum. II. Cytogenetic studies

1984 ◽  
Vol 26 (6) ◽  
pp. 736-742 ◽  
Author(s):  
R. P. Singh ◽  
R. A. McIntosh
Keyword(s):  
Triticum Aestivum ◽  
Leaf Rust ◽  
Puccinia Recondita ◽  
Monosomic Analysis ◽  
Complementary Genes ◽  
Cytogenetic Studies ◽  
Puccinia Recondita Tritici

Two complementary genes, A and B, conferring resistance to Puccinia recondita tritici in various wheats were located in chromosomes 4Aβ and 3BS, respectively. In one study gene B showed recombination of 33.6 ± 4.1% with the centromere, and was independent in a second study. Gene B was the same as that designated Lr27. A new designation, Lr31, is proposed for gene A. Both Lr27 and Lr31 must be present for the expression of resistance.Key words: leaf rust, monosomic analysis, aneuploids, wheat.

C-banding at meiosis as a means of analyzing cytogenetic structure in wheat

1985 ◽  
Vol 27 (6) ◽  
pp. 689-696 ◽  
Author(s):  
A. Fominaya ◽  
N. Jouve
Keyword(s):  
Quantitative Analysis ◽  
Chinese Spring ◽  
Monosomic Analysis ◽  
C Banding ◽  
Staining Techniques

The characterization of reciprocal interchanges between chromosomes of wheat in the progeny of the backcross ((T. aestivum H-53 × S. cereale) × T. aestivum H-53) using Feulgen and C-banding staining techniques has been attempted. The translocated chromosomes were studied in detail in three lines using monosomic analysis. In the F1 of the crosses between these lines and the 21 monosomic lines of 'Chinese Spring' a total of five interchanges were identified. Using a Giemsa C-banding procedure it was possible to confirm the identity of the interchange chromosome pairs that were previously identified by monosomic analysis. Moreover, a total of 12 new interchanges were characterized or identified. C-banding also facilitated the identification of the arms involved in translocations and permitted the quantitative analysis of each multivalent in multiple interchange heterozygotes. The comparative availability of Feulgen and C-banding in studies of cytogenetic structure in wheat is discussed.Key words: C-banding, meiosis, heterochromatin, translocations, wheat, Triticum.

CYTOGENETICS OF SOLID STEM IN COMMON WHEAT: III. CULM MEASUREMENTS AND THEIR RELATION TO STEM SOLIDNESS IN MONOSOMIC LINES OF THE VARIETY S-615

1959 ◽  
Vol 37 (3) ◽  
pp. 379-391 ◽  
Author(s):  
Ruby I. Larson ◽  
M. D. MacDonald
Keyword(s):  
Common Wheat ◽  
Chinese Spring ◽  
Monosomic Analysis ◽  
Secondary Effects ◽  
Stem Solidness ◽  
Solid Stem ◽  
Homoeologous Chromosomes

Most monosomics of the solid-stemmed variety of common wheat, S-61S, were shorter than normal. Many had thinner culms, but monosomics XIII and XVI had thicker culms. Although the effect of loss of homoeologous chromosomes on culm dimensions was generally similar in S-615 monosomics and Chinese Spring nullisomics, the most extreme member of each group was not always the same in the two varieties. The differences between solid stem in monosomics and normal lines of S-615 were due to loss of the chromosome affecting pith production and not to secondary effects caused by changes in culm dimensions. Nevertheless, within lines of genetically similar plants there was a small but consistent association between short culm and solidness, between thin culm at the tops of internodes I, III, and IV and hollowness, and between thin culm in the center and lower parts of internode I and solidness. This has a bearing on interpretation of data in monosomic analysis of solid stem.

scholarly journals Cytogenetic Analysis of Two Chromosomal Male-sterility Mutants in Hexaploid Wheat

1975 ◽  
Vol 28 (4) ◽  
pp. 413 ◽  
Author(s):  
CJ Driscoll
Keyword(s):  
Male Sterility ◽  
Hexaploid Wheat ◽  
Cytogenetic Analysis ◽  
Monosomic Analysis ◽  
Male Sterile

The two chromosomal male-sterility mutants referred to as Pugsley's male-sterile and the Probus male-sterile fail to complement one another. Partial monosomic analysis revealed that both mutants are located on chromosome 4A. A strong homoeoallelic 'echo' was received from chromosome 4B with the Probus mutant, mslb, but not with Pugsley's mutant, msla.

Monosomic analysis reveals duplicated chromosomal segments in maize genome

2009 ◽  
Vol 88 (3) ◽  
pp. 331-335
Author(s):  
Mahesh C. Yadav ◽  
J. K. S. Sachan ◽  
K. R. Sarkar
Keyword(s):  
Monosomic Analysis ◽  
Maize Genome

Chromosomal location by F1 monosomic analysis of endosperm proteins in bread wheat

1988 ◽  
Vol 76 (6) ◽  
pp. 933-940 ◽  
Author(s):  
J. C. Sanz ◽  
G. Hueros ◽  
J. M. Gonzalez ◽  
N. Jouve
Keyword(s):  
Bread Wheat ◽  
Chromosomal Location ◽  
Monosomic Analysis ◽  
Endosperm Proteins

A gene for resistance to a necrosis-inducing isolate of Pyrenophora tritici-repentis located on 5BL of Triticum aestivum cv. Chinese Spring

Genome ◽  
1996 ◽  
Vol 39 (3) ◽  
pp. 598-604 ◽  
Author(s):  
W. S. Stock ◽  
A. L. Brûlé-Babel ◽  
G. A. Penner
Keyword(s):  
Triticum Aestivum ◽  
Resistance Gene ◽  
Chinese Spring ◽  
Recessive Gene ◽  
Triticum Aestivum L ◽  
Tan Spot ◽  
Monosomic Analysis ◽  
Chromosome Location ◽  
Pyrenophora Tritici Repentis ◽  
Chromosome Arm

Several sources of high-level resistance to tan spot caused by Pyrenophora tritici-repentis have been identified in hexaploid wheat (Triticum aestivum L.). This study was conducted to determine the number and chromosome location of a gene(s) in the cultivar Chinese Spring (CS) that confers resistance to a tan necrosis inducing isolate (nec+chl−) of P. tritici-repentis, 86-124, and insensitivity to Ptr necrosis toxin. Reciprocal crosses were made between CS (resistant–insensitive) and 'Kenya Farmer' (KF) (susceptible–sensitive). Analysis of the CS/KF F1and F2 populations and F2-derived F3 families identified a single nuclear recessive gene governing resistance to isolate 86-124 and Ptr necrosis toxin. Evaluation of the CS(KF) substitution series, F2 monosomic analysis, and screening of a series of 19 CS compensating nullitetrasomic and two ditelosomic lines (2AS and 5BL) indicated that the resistance gene was located on chromosome arm 5BL. No linkage exists between Lr18 and the tan necrosis resistance gene on chromosome arm 5BL. It is proposed that the gene for resistance to the tan necrosis inducing isolate 86-124 (nec+chl−) of P. tritici-repentis and Ptr necrosis toxin be named tsn1. Key words : wheat, Triticum aestivum L., tan spot resistance, Pyrenophora tritici-repentis (Died.) Drechs., chromosome location, Ptr necrosis toxin.

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