Scanning electron microscopy of organic structures on the root surface of human teeth

1974 ◽  
Vol 66 (2) ◽  
pp. 221
2005 ◽  
Vol 13 (3) ◽  
pp. 247-252 ◽  
Author(s):  
José Eduardo Cezar Sampaio ◽  
Flávia Pavan Campos ◽  
Gibson Luiz Pilatti ◽  
Letícia Helena Theodoro ◽  
Fábio Renato Manzolli Leite

The aim of the present study was to compare root surface smear layer removal following topical application of EDTA and EDTA-T (Texapon). Extracted human teeth had their cementum removed and were mechanically scaled. A total of 220 root specimens were obtained and were randomly assigned to the following groups: I-saline solution (control), II-EDTA; III-EDTA-T. Groups II and III specimens were assigned to different EDTA gel concentrations: 5%, 10%, 15%, 20% and 24%. Smear layer removal score was assessed for each specimen by scanning electron microscopy. The results demonstrated that EDTA and EDTA-T gel led to a higher root surface smear layer removal when compared to the control group. The 5% EDTA gel also showed a higher smear layer removal than the 15%, 20% and 24% EDTA gels (p<0.05). No difference could be found between the different concentrations of EDTA-T gels tested (p>0.05). EDTA gels had statistically significantly lower smear layer scores than the EDTA-T gels for the 5% and 10% concentrations. The results suggested that topical application of EDTA or EDTA-T gel led to significant smear layer removal of the mechanically treated root surfaces. The addition of a detergent to the EDTA gel formula did not improve smear layer removal of the root surface.


e-Polymers ◽  
2005 ◽  
Vol 5 (1) ◽  
Author(s):  
Joanna Siejka-Kulczyk ◽  
Małgorzata Lewandowska ◽  
Marta Raczyńska ◽  
Elżbieta Jodkowska ◽  
Krzysztof J. Kurzydłowski

AbstractScanning electron microscopy (SEM) investigations have been conducted to determine the influence of selected material on tissue/material bonding. Extracted human teeth were used in this study. Several resin composites were tested as dental fillings. To reveal the structure of the tissue/filling interface, the teeth were cut parallel to the long axis. Bonding quality was determined by direct SEM observation and microanalysis with X-ray energy spectrometry (EDX). The investigated materials show good bonding with enamel.


2020 ◽  
Vol 10 (18) ◽  
pp. 6513
Author(s):  
Emilia Bologa ◽  
Simona Stoleriu ◽  
Gianina Iovan ◽  
Cristina Angela Ghiorghe ◽  
Irina Nica ◽  
...  

This in vitro study evaluated the effects of dentifrices containing nano-hydroxyapatite (n-HAp) on dentinal tubule occlusion and on mineral deposition. Dentin specimens of ten human teeth were submersed for 30 s in 40% citric acid and then randomly divided into four groups (three study groups and one control group). In the study groups, the dentin samples were exposed to three different n-HAp toothpastes: Karex (Dr. Kurt Wolff GmbH & Co. KG, Bielefeld, Germany), Biorepair Plus Sensitive (Coswell SpA, Bologna, Italy), and Dr. Wolff’s Biorepair (Dr. Kurt Wolff GmbH & Co. KG, Bielefeld, Germany); in the control group no toothpaste was applied. All of the samples were evaluated using scanning electron microscopy (SEM) and energy-dispersive X-ray (EDX) analysis. In the control group all of the samples showed a frank and wide opening of the dentinal tubules, whereas in the study groups different degrees of tubule closure by mineral depositions were observed. Toothpastes containing n-HAp determined a significant occlusion of dentinal tubules and a significant increase of mineral deposition on the dentin surface. All three tested toothpastes showed similar results regarding the degree of dentinal tubule closure. Varying degrees of differences in calcium, phosphate, carbon, and oxygen ion concentrations among the three tested toothpastes were obtained.


2007 ◽  
Vol 31 (4) ◽  
pp. 321-325 ◽  
Author(s):  
Mário Roberto Leonardo ◽  
Marcos Antonio Rossi ◽  
Kleber Cortês Bonifácio ◽  
Léa Assed Bezerra da Silva ◽  
Sada Assed

2015 ◽  
Vol 21 (5) ◽  
pp. 1264-1270 ◽  
Author(s):  
Manila Chieruzzi ◽  
Stefano Pagano ◽  
Carlo De Carolis ◽  
Stefano Eramo ◽  
José M. Kenny

AbstractThe inflammatory resorption of dental root apex (i.e., the process of removal of cementum and/or dentine through the activity of resorbing cells) may show different configurations and damage the apical root structure. As knowing the morphology of resorption areas of human teeth is essential for the success of endodontic treatments, we investigated the apical resorption by scanning electron microscopy, focusing on roots with granulomas. A total of 30 teeth (with penetrating carious lesions and chronic periapical lesions) were examined, the apical third of the roots were removed and analyzed to estimate periforaminal and foraminal resorption, shape and morphology of foramen resorption, centering of the periforaminal resorption area, and diameters of each apical foramen. Periforaminal resorption was present in all samples, whereas foraminal resorption was present in 92% of cases (mainly funnel shaped). Lacunae were observed in the foraminal resorption area with an average diameter of 35±14 μm. The major and minor diameters of the foramina in teeth with resorption were 443 and 313 μm, respectively (higher than in healthy teeth). This result indicates an expansion of the apical diameters caused by the pathology, which could encourage a different clinical instrumentation for these teeth.


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