Continuous scintillation counting of carbon-14 and tritium in effluent of the automatic amino acid analyzer

By using the automatic amino acid analyzer (model 120 B, Beckman) the free ninhydrin-positive components in the methanol extracts of Drosophila melanogaster were fractionated. Compared to the conventional paper partition chromatography the great advantage of this technique is the distinct separation of such amino acids like leucine, isoleucine, phenylalanine, valine, methionine, γ-amino-butyric acid as well as the basic components ornithine, lysine, histidine and arginine. Furthermore, the occurrence of phosphoserine, tyrosine phosphate, ethanolamine, phosphoethanolamine and glycerophosphoethanolamine was identified. The metabolic significance of these substances is discussed.

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A method for the improved resolution of basic amino acids on the automatic amino acid analyzer

Journal of Chromatography A ◽

10.1016/s0021-9673(00)80775-x ◽

1962 ◽

Vol 9 ◽

pp. 253-254 ◽

Cited By ~ 20

Author(s):

David R. Kominz

Keyword(s):

Amino Acids ◽

Amino Acid ◽

Automatic Amino Acid Analyzer ◽

Basic Amino Acids ◽

Amino Acid Analyzer

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Continuous Scintillation Counting of Amino Acid Analyzer Column Eluates

Advances in Tracer Methodology ◽

10.1007/978-1-4684-8622-3_12 ◽

1965 ◽

pp. 115-122 ◽

Cited By ~ 2

Author(s):

David H. Elwyn

Keyword(s):

Amino Acid ◽

Scintillation Counting ◽

Amino Acid Analyzer

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Comparative Analysis of Free Amino Acids in Parapenaeopsis hardwickii, Penaeus vannamei and Macrobrachium nipponensis

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.781-784.1528 ◽

2013 ◽

Vol 781-784 ◽

pp. 1528-1533

Author(s):

Yi Hua Zhang ◽

Shun Sheng Chen ◽

Wei Qiang Qiu ◽

Shou Kun Cheng

Keyword(s):

Amino Acids ◽

Comparative Analysis ◽

Amino Acid ◽

Free Amino Acids ◽

Free Amino ◽

Penaeus Vannamei ◽

High Concentration ◽

Automatic Amino Acid Analyzer ◽

Low Concentration ◽

Amino Acid Analyzer

The contents of free amino acids(FAAs) in Parapenaeopsis hardwickii, Penaeus vannamei and Macrobrachium nipponensis was analyzed by using the automatic amino acid analyzer in this study. The results show that the sequence from the highest to the lowest in total amount of FAAs is Parapenaeopsis hardwickii, Penaeus vannamei and Macrobrachium nipponensis. SPSS(19.0) results indicate that except for Cys, Lys and Thr, FAAs in these three shrimps are significant different (p<0.05), and all of them have a high concentration of Arg, Gly and Pro and a low concentration of Asp and Cys. The content of Gly in shrimp is higher than that of crab and oyster. Both Arg and Gly significantly contribute to the taste of the three shrimps, Glu and Pro play an important role in the flavor of Parapenaeopsis hardwickii and Penaeus vannamei, while not as significant as in the taste of Macrobrachium nipponensis. Composition modes of FAAs which make major contributions to flavor in marine shrimps, Parapenaeopsis hardwickii and Penaeus vannamei, are similar, but different from freshwater shrimp, Macrobrachium nipponensis.

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