Separation of citrate, malate, aspartate, and glutamate in crude extracts by one-dimensional ion exchange thin-layer chromatography

1978 ◽  
Vol 88 (2) ◽  
pp. 634-637 ◽  
Author(s):  
Albert Himoe ◽  
Robert W. Rinne
Keyword(s):  
Ion Exchange ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
One Dimensional ◽  
Crude Extracts ◽  
Layer Chromatography

Amniotic fluid phospholipids measured by continuous-development thin-layer chromatography.

1980 ◽  
Vol 26 (3) ◽  
pp. 403-405
Author(s):  
M D Kolins ◽  
E Epstein ◽  
W H Civin ◽  
S Weiner
Keyword(s):  
Thin Layer ◽  
Amniotic Fluid ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Chromatographic System ◽  
Continuous Development ◽  
One Dimensional ◽  
Layer Chromatography

Abstract We describe a one-dimensional thin-layer chromatographic system for separation of amniotic fluid phosphatidylcholine, sphingomyelin, phosphatidylglycerol, phosphatidylinositol, phosphatidylserine, and phosphatidylethanolamine in amniotic fluid. We utilize short-bed continuous development and "high performance" thin-layer chromatography. Phospholipids are detected with an antimony molybdate staining reagent and quantitated by transmittance densitometry. This system is more sensitive to changes in lecithin/sphingomyelin ratios than are planimetric evaluations.

Detection of PG, NDGA, BHT, and BHA in Fats and Oils by Thin Layer Chromatography

1966 ◽  
Vol 49 (4) ◽  
pp. 807-809
Author(s):  
Shirley A Scheidt ◽  
Harry W Conroy
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Silica Gel ◽  
Chromatographic Separation ◽  
Fats And Oils ◽  
Adsorbent Layer ◽  
One Dimensional ◽  
Layer Chromatography

Abstract PG, NDGA, BHT, and BHA were extracted with acetonitrile from fat or oil, followed by thin layer chromatographic separation and identification. The four antioxidants were distinctly separated from one another by one-dimensional solvent development of the silica gel adsorbent layer. The chromatography required < 1 hour from spotting to visualization with 2,6-dichloroquinone chlorimide. As little as 1 μg of BHT and 0.5 μg of PG, NDGA, and BHA were detected by this method.

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