Densitometric quantitation of individual phospholipids from natural sources separated by one-dimensional thin-layer chromatography

1984 ◽  
Vol 140 (1) ◽  
pp. 152-156 ◽  
Author(s):  
Margarete Goppelt ◽  
Klaus Resch
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Natural Sources ◽  
One Dimensional ◽  
Layer Chromatography

Amniotic fluid phospholipids measured by continuous-development thin-layer chromatography.

1980 ◽  
Vol 26 (3) ◽  
pp. 403-405
Author(s):  
M D Kolins ◽  
E Epstein ◽  
W H Civin ◽  
S Weiner
Keyword(s):  
Thin Layer ◽  
Amniotic Fluid ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Chromatographic System ◽  
Continuous Development ◽  
One Dimensional ◽  
Layer Chromatography

Abstract We describe a one-dimensional thin-layer chromatographic system for separation of amniotic fluid phosphatidylcholine, sphingomyelin, phosphatidylglycerol, phosphatidylinositol, phosphatidylserine, and phosphatidylethanolamine in amniotic fluid. We utilize short-bed continuous development and "high performance" thin-layer chromatography. Phospholipids are detected with an antimony molybdate staining reagent and quantitated by transmittance densitometry. This system is more sensitive to changes in lecithin/sphingomyelin ratios than are planimetric evaluations.

Detection of PG, NDGA, BHT, and BHA in Fats and Oils by Thin Layer Chromatography

1966 ◽  
Vol 49 (4) ◽  
pp. 807-809
Author(s):  
Shirley A Scheidt ◽  
Harry W Conroy
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Silica Gel ◽  
Chromatographic Separation ◽  
Fats And Oils ◽  
Adsorbent Layer ◽  
One Dimensional ◽  
Layer Chromatography

Abstract PG, NDGA, BHT, and BHA were extracted with acetonitrile from fat or oil, followed by thin layer chromatographic separation and identification. The four antioxidants were distinctly separated from one another by one-dimensional solvent development of the silica gel adsorbent layer. The chromatography required < 1 hour from spotting to visualization with 2,6-dichloroquinone chlorimide. As little as 1 μg of BHT and 0.5 μg of PG, NDGA, and BHA were detected by this method.

Analysis of lipids by one-dimensional thin-layer chromatography

1987 ◽  
Vol 387 ◽  
pp. 323-331 ◽  
Author(s):  
Azam A. Entezami ◽  
Barney J. Venables ◽  
Kenneth E. Daugherty
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
One Dimensional ◽  
Layer Chromatography

Two-Dimensional Thin-Layer Chromatography on Two-Layer Plates of Amino Acids

1970 ◽  
Vol 16 (8) ◽  
pp. 662-666 ◽  
Author(s):  
F Kraffczyk ◽  
R Helger ◽  
H Lang
Keyword(s):  
Amino Acids ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Two Dimensions ◽  
Two Dimensional ◽  
One Dimensional ◽  
Acid Cation ◽  
Solvent Systems ◽  
Strong Acids ◽  
Layer Chromatography

Abstract Separation of the amino acids in urine by use of thin-layer chromatography (TLC) has hitherto required that the specimen be first desalted and then chromatographed in two dimensions with at least two pairs of developing solvent systems. We wished to simplify both steps. The customary method of desalting on a column is replaced by desalting on a plate that supports a strongly acid cation-exchanger and a cellulose layer. This method, originally developed for one-dimensional TLC, is used here for two-dimensional TLC. Urine is applied to the ion-exchange layer and strong acids and neutral substances are removed with water. The amino acids are then chromatographed into the cellulose layer, and are separated there two dimensionally with a newly devised pair of developing solutions. This pair of solvents separates nearly all of the amino acids in urine.

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