An electrochemical approach to quantitation and characterization of metallothioneins

1979 ◽  
Vol 100 (2) ◽  
pp. 343-351 ◽  
Author(s):  
R.W. Olafson ◽  
R.G. Sim
Keyword(s):  
Electrochemical Approach

scholarly journals An Electrochemical Approach to Follow and Evaluate the Kinetic Catalysis of Ricin on hsDNA

Life ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 405
Author(s):  
George Oliveira ◽  
José Maurício Schneedorf
Keyword(s):  
Catalytic Efficiency ◽  
Ricin Toxin ◽  
Wave Voltammetry ◽  
Progress Curve ◽  
Electrochemical Approach ◽  
Electro Oxidation ◽  
Castor Seeds ◽  
Identification And Characterization ◽  
Medical Countermeasures

International authorities classify the ricin toxin, present in castor seeds, as a potential agent for use in bioterrorism. Therefore, the detection, identification, and characterization of ricin are considered the first actions for its risk assessment during a suspected exposure, parallel to the development of therapeutic and medical countermeasures. In this study, we report the kinetic analysis of electro-oxidation of adenine released from hsDNA by the catalytic action of ricin by square wave voltammetry. The results suggest that ricin-mediated adenine release exhibited an unusual kinetic profile, with a progress curve controlled by the accumulation of the product and the values of the kinetic constants of 46.6 µM for Km and 2000 min−1 for kcat, leading to a catalytic efficiency of 7.1 × 105 s−1 M−1.

scholarly journals An Efficient Electrochemical Approach for Characterization of the Interface Strength of Nano Titania-Silica (TS) Composite Coating on Titanium Implants

2018 ◽  
Vol 34 (4) ◽  
pp. 2180-2186
Author(s):  
Suja Mathai
Keyword(s):  
Composite Coating ◽  
Alkaline Treatment ◽  
Interface Strength ◽  
Titanium Implants ◽  
Anodic Current ◽  
Thermal Decomposition Process ◽  
Electrochemical Approach ◽  
Topographical Analysis ◽  
Sbf Solution

The thermal decomposition process developed in the present work is a viable technique for the formation of a nano TS (TiO2-SiO2) composite coating on Ti substrate. The electrochemical experiments revealed that the developed coatings had high degradation resistance characteristics and high biomimetic growth characteristics. There was no degradation on the coating even when it was subjected to different extent of electrochemical polarization and anodic current impression. The biomimetic growth studies of these coatings were carried out without any alkaline treatment. Surface and topographical analysis including SEM and AFM were also carried after biomimetic growth. The results of elemental analysis of the SBF solution and electrochemical characterization carried out at different stages revealed that there was efficient apatite growth even without pre-alkaline treatment.

Characterization of Two Quinone Radicals in the NADH:Ubiquinone Oxidoreductase from Escherichia coli by a Combined Fluorescence Spectroscopic and Electrochemical Approach

Biochemistry ◽  
2013 ◽  
Vol 52 (50) ◽  
pp. 8993-9000 ◽  
Author(s):  
Ruth Hielscher ◽  
Michelle Yegres ◽  
Mariana Voicescu ◽  
Emmanuel Gnandt ◽  
Thorsten Friedrich ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Nadh:Ubiquinone Oxidoreductase ◽  
Electrochemical Approach

Morphological Characterization of Mycobacteriophage R1

1974 ◽  
Vol 32 ◽  
pp. 254-255
Author(s):  
B. L. Soloff ◽  
T. A. Rado
Keyword(s):  
Carbon Source ◽  
Stationary Phase ◽  
Growth Phase ◽  
Minimal Medium ◽  
Morphological Characterization ◽  
Logarithmic Growth Phase ◽  
Complete Lysis ◽  
Lysogenic Culture ◽  
Logarithmic Growth

Mycobacteriophage R1 was originally isolated from a lysogenic culture of M. butyricum. The virus was propagated on a leucine-requiring derivative of M. smegmatis, 607 leu−, isolated by nitrosoguanidine mutagenesis of typestrain ATCC 607. Growth was accomplished in a minimal medium containing glycerol and glucose as carbon source and enriched by the addition of 80 μg/ ml L-leucine. Bacteria in early logarithmic growth phase were infected with virus at a multiplicity of 5, and incubated with aeration for 8 hours. The partially lysed suspension was diluted 1:10 in growth medium and incubated for a further 8 hours. This permitted stationary phase cells to re-enter logarithmic growth and resulted in complete lysis of the culture.

AEM analysis of crystallization in Ti-based amorphous alloys

1983 ◽  
Vol 41 ◽  
pp. 270-271
Author(s):  
A.R. Pelton ◽  
A.F. Marshall ◽  
Y.S. Lee
Keyword(s):  
Magnetic Properties ◽  
Amorphous Alloys ◽  
Amorphous Materials ◽  
Isothermal Annealing ◽  
Amorphous Matrix ◽  
Nucleation And Growth ◽  
Current Interest ◽  
Amorphous Films ◽  
Electrical And Magnetic Properties

Amorphous materials are of current interest due to their desirable mechanical, electrical and magnetic properties. Furthermore, crystallizing amorphous alloys provides an avenue for discerning sequential and competitive phases thus allowing access to otherwise inaccessible crystalline structures. Previous studies have shown the benefits of using AEM to determine crystal structures and compositions of partially crystallized alloys. The present paper will discuss the AEM characterization of crystallized Cu-Ti and Ni-Ti amorphous films.Cu60Ti40: The amorphous alloy Cu60Ti40, when continuously heated, forms a simple intermediate, macrocrystalline phase which then transforms to the ordered, equilibrium Cu3Ti2 phase. However, contrary to what one would expect from kinetic considerations, isothermal annealing below the isochronal crystallization temperature results in direct nucleation and growth of Cu3Ti2 from the amorphous matrix.

Characterization of Coherent, Ordered Precipitates in Nickel-Base Alloys

1973 ◽  
Vol 31 ◽  
pp. 144-145
Author(s):  
B. H. Kear ◽  
J. M. Oblak
Keyword(s):  
Stable Phase ◽  
Nickel Base Superalloy ◽  
Alloy 718 ◽  
Commercial Alloy ◽  
Precipitate Morphology ◽  
Continuous Precipitation ◽  
Nickel Base ◽  
Base Superalloy ◽  
Strengthening Precipitate

A nickel-base superalloy is essentially a Ni/Cr solid solution hardened by additions of Al (Ti, Nb, etc.) to precipitate a coherent, ordered phase. In most commercial alloy systems, e.g. B-1900, IN-100 and Mar-M200, the stable precipitate is Ni3 (Al,Ti) γ′, with an LI2structure. In A lloy 901 the normal precipitate is metastable Nis Ti3 γ′ ; the stable phase is a hexagonal Do2 4 structure. In Alloy 718 the strengthening precipitate is metastable γ″, which has a body-centered tetragonal D022 structure.Precipitate MorphologyIn most systems the ordered γ′ phase forms by a continuous precipitation re-action, which gives rise to a uniform intragranular dispersion of precipitate particles. For zero γ/γ′ misfit, the γ′ precipitates assume a spheroidal.

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