Specific binding sites for 125I-nerve growth factor in peripheral tissues and brain

1974 ◽  
Vol 57 (4) ◽  
pp. 1096-1103 ◽  
Author(s):  
William A. Frazier ◽  
Linda F. Boyd ◽  
Andrzej Szutowicz ◽  
Morris W. Pulliam ◽  
Ralph A. Bradshaw
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Binding Sites ◽  
Specific Binding ◽  
Peripheral Tissues ◽  
Nerve Growth ◽  
Specific Binding Sites

The effect of nerve growth factor on the development of sodium channels in PC12 cells

1986 ◽  
Vol 64 (11) ◽  
pp. 1153-1159 ◽  
Author(s):  
Juta K. Reed ◽  
Diane England
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Pc12 Cells ◽  
Specific Binding ◽  
Neuronal Cell ◽  
Scorpion Toxin ◽  
Excitable Cells ◽  
Response Curves ◽  
Nerve Growth ◽  
Differentiated Cells

We have studied the development of the action potential Na+ channels in PC12 cells, an established line that has been useful as a model for neuronal differentiation. In continuous culture PC12 cells, although electrically inexcitable, nevertheless have a low level of Na+ channels as judged by the increase in 22Na+ uptake in the presence of veratridine and scorpion toxin. These two neurotoxins have been shown to promote activation of Na+ channels in a variety of electrically excitable cells. Following treatment with nerve growth factor (NGF), conditions which induce differentiation to an electrically excitably neuronal-cell type, the neurotoxin-activated 22Na+ uptake increases approximately 12-fold, on a per cell basis, reaching a maximum in 12–16 days. The dose–response curves for veratridine and scorpion toxin are unchanged by NGF treatment (K0.5 for veratridine, 18–14 μM; K0.5 for scorpion toxin, 120–96 nM). Na+ channels in both undifferentiated and differentiated cells are tetrodotoxin sensitive and NGF treatment has no effect on the inhibition constant (Ki, 10–12 nM). Na+ channel sites were measured directly by the specific binding of [3H]saxitoxin. In NGF-treated cells, the saxitoxin receptor density reaches 154 fmol/mg protein (Kd, 1.3 nM), a level comparable to other excitable cells. Levels in control cells were too low to measure accurately. These findings show that NGF treatment of PC12 cells leads to a substantial increase in the expression of neurotoxin-sensitive Na+ channels. Furthermore, these channels are pharmacologically similar, if not identical, to those which exist in undifferentiated cells and therefore do not appear to result from the conversion of preexisting channels.

Identification of cells in rat brain and peripheral tissues expressing mRNA for members of the nerve growth factor family

Neuron ◽  
1990 ◽  
Vol 5 (4) ◽  
pp. 511-526 ◽  
Author(s):  
Patrik Ernfors ◽  
Cynthia Wetmore ◽  
Lars Olson ◽  
Håkan Persson
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Rat Brain ◽  
Peripheral Tissues ◽  
Nerve Growth

Nerve growth factor binding sites on hepatic parenchymal cells

1992 ◽  
Vol 28 (9-10) ◽  
pp. 621-624 ◽  
Author(s):  
M. B. Bailie ◽  
R. A. Roth ◽  
M. L. Contreras
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Binding Sites ◽  
Nerve Growth ◽  
Factor Binding ◽  
Parenchymal Cells
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