A phase/quadrature correlator for the measurement of fluorescence-decay times by single-photon counting

1991 ◽  
Vol 179 (3) ◽  
pp. 211-217 ◽  
Author(s):  
C.G. Morgan ◽  
J.G. Murray
Keyword(s):  
Single Photon ◽  
Photon Counting ◽  
Fluorescence Decay ◽  
Single Photon Counting ◽  
Decay Times ◽  
Fluorescence Decay Times

Single Particle Spectroscopy of Radiative Processes in Colloid-to-Film-Coupled Nanoantennas

2018 ◽  
Vol 232 (9-11) ◽  
pp. 1593-1606 ◽  
Author(s):  
Max J. Schnepf ◽  
Yannic Brasse ◽  
Fabian R. Goßler ◽  
Anja Maria Steiner ◽  
Julian Obermeier ◽  
...  
Keyword(s):  
Single Particle ◽  
Radiative Decay ◽  
Single Photon ◽  
Photon Counting ◽  
Life Time ◽  
Fluorescence Decay ◽  
Decay Rates ◽  
Single Photon Counting ◽  
Radiative Processes ◽  
Particle Level

Abstract We present a fluorescent emitter (rhodamine B) coupled to a dielectric or metallic interface as well as a metallic cavity to study their radiative decay processes. Supported by finite-difference time-domain (FDTD) simulations, we correlate the non-radiative and radiative decay rates with the absorption and scattering cross section efficiencies, respectively. On a single particle level, we use atomic force microscopy (AFM), scanning electron microscopy (SEM), scattering spectroscopy, fluorescence life time imaging (FLIM) and time-correlated single photon counting (TCSPC) to evaluate the enhanced fluorescence decay at the same location. With this study, we show a colloidal gain material, which can be integrated into lattices using existing directed self-assembled methods to study their coherent energy transfer.

Picosecond Tryptophan Fluorescence of Human Blood Serum Orosomucoid

1996 ◽  
Vol 61 (5) ◽  
pp. 808-818 ◽  
Author(s):  
Martin Hof ◽  
Stefan Vajda ◽  
Vlastimil Fidler ◽  
Vladimír Karpenko
Keyword(s):  
Single Photon ◽  
Global Analysis ◽  
Photon Counting ◽  
Tryptophan Fluorescence ◽  
Fluorescence Decay ◽  
Amino Acid Residues ◽  
Counting System ◽  
Single Photon Counting ◽  
Decay Behaviour ◽  
The Individual

The state of three tryptophyl residues in human serum orosomucoid was estimated by prediction methods based on parameters characterizing their hydrophobicity either directly, or in terms of buried surfaces of the individual amino acid residues. It is shown that tryptophan 25 is the most buried, while Trp 160 is the most exposed to the solvent. Trp 122 is in this respect in an intermediate state. The fluorescence decay behaviour was determined using a picosecond single photon counting system. The multiwavelength data were analyzed using a global analysis as well as a distribution of lifetimes program. Both procedures yielded the existence of four wavelength independent lifetimes (0.22 ns, 1.0 ns, 2.5 ns, and 8.4 ns). A tentative assignment of the decay associated spectra of the four components to the three individual tryptophans is presented.

Measurement of fluorescence decay curves by the single photon counting method

1981 ◽  
Vol 34 (2) ◽  
pp. 165-168
Author(s):  
A. B. Evdokimov ◽  
G. V. Lukomskii ◽  
B. S. Neporent ◽  
O. V. Stolbova
Keyword(s):  
Single Photon ◽  
Photon Counting ◽  
Fluorescence Decay ◽  
Counting Method ◽  
Single Photon Counting ◽  
Photon Counting Method

Measurement System of Decay Time for Liquid Scintillator

2014 ◽  
Vol 513-517 ◽  
pp. 4285-4286
Author(s):  
Li Ping He ◽  
Yu Fang ◽  
Shu Fan Chen ◽  
Xuan Luo ◽  
Zhi Jun Wei ◽  
...  
Keyword(s):  
Excited States ◽  
Fluorescence Lifetime ◽  
Pulse Shape ◽  
Single Photon ◽  
Uv Light ◽  
Liquid Scintillator ◽  
Fluorescence Decay ◽  
Decay Times ◽  
Light Excitation ◽  
Fluorescence Decay Times

The fluorescence lifetime of the molecular excited states in the liquid scintillator determines the pulse shape of the events in a detector. The fluorescence decay times of the mixtures could be measured by UV light excitation, or via ionizing particles. Both of the setups are based on the time-correlated single photon technique. In this paper we described a setup in detail for testing the fluorescence lifetime under ionizing particle to render the relatively weak neutron or ion signals detectable. In addition, we presented the time resolution of system for the setup.

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