On the Application of the Single Photon Counting Technique for the Investigation of Dyes with Short Fluorescence Decay Time

1973 ◽  
pp. 206-212 ◽  
Author(s):  
P. E. Zinsli ◽  
H. P. Tschanz ◽  
O. Jenni ◽  
Th. Binkert
Keyword(s):  
Decay Time ◽  
Single Photon ◽  
Photon Counting ◽  
Fluorescence Decay ◽  
Single Photon Counting ◽  
Counting Technique ◽  
Fluorescence Decay Time

Single Particle Spectroscopy of Radiative Processes in Colloid-to-Film-Coupled Nanoantennas

2018 ◽  
Vol 232 (9-11) ◽  
pp. 1593-1606 ◽  
Author(s):  
Max J. Schnepf ◽  
Yannic Brasse ◽  
Fabian R. Goßler ◽  
Anja Maria Steiner ◽  
Julian Obermeier ◽  
...  
Keyword(s):  
Single Particle ◽  
Radiative Decay ◽  
Single Photon ◽  
Photon Counting ◽  
Life Time ◽  
Fluorescence Decay ◽  
Decay Rates ◽  
Single Photon Counting ◽  
Radiative Processes ◽  
Particle Level

Abstract We present a fluorescent emitter (rhodamine B) coupled to a dielectric or metallic interface as well as a metallic cavity to study their radiative decay processes. Supported by finite-difference time-domain (FDTD) simulations, we correlate the non-radiative and radiative decay rates with the absorption and scattering cross section efficiencies, respectively. On a single particle level, we use atomic force microscopy (AFM), scanning electron microscopy (SEM), scattering spectroscopy, fluorescence life time imaging (FLIM) and time-correlated single photon counting (TCSPC) to evaluate the enhanced fluorescence decay at the same location. With this study, we show a colloidal gain material, which can be integrated into lattices using existing directed self-assembled methods to study their coherent energy transfer.

Image quality in optical imaging by time-correlated single photon-counting technique

2005 ◽  
Author(s):  
M. Mormile ◽  
R. Esposito ◽  
R. Romano ◽  
M. Lepore ◽  
P. L. Indovina
Keyword(s):  
Image Quality ◽  
Optical Imaging ◽  
Single Photon ◽  
Photon Counting ◽  
Single Photon Counting ◽  
Counting Technique

scholarly journals Probing DNA-stabilized fluorescent silver nanocluster spectral heterogeneity by time-correlated single photon counting

The Analyst ◽  
2016 ◽  
Vol 141 (1) ◽  
pp. 123-130 ◽  
Author(s):  
Miguel R. Carro Temboury ◽  
Valentina Paolucci ◽  
Emma N. Hooley ◽  
Loredana Latterini ◽  
Tom Vosch
Keyword(s):  
Sample Preparation ◽  
Decay Time ◽  
Single Photon ◽  
Photon Counting ◽  
Silver Nanoclusters ◽  
Preparation Procedure ◽  
Single Photon Counting ◽  
Silver Nanocluster ◽  
Sample Preparation Procedure

Average decay time spectra can be used as a tool to characterize the spectral heterogeneity of DNA templated silver nanoclusters (DNA-AgNCs) and assess the sample preparation procedure.

Picosecond Tryptophan Fluorescence of Human Blood Serum Orosomucoid

1996 ◽  
Vol 61 (5) ◽  
pp. 808-818 ◽  
Author(s):  
Martin Hof ◽  
Stefan Vajda ◽  
Vlastimil Fidler ◽  
Vladimír Karpenko
Keyword(s):  
Single Photon ◽  
Global Analysis ◽  
Photon Counting ◽  
Tryptophan Fluorescence ◽  
Fluorescence Decay ◽  
Amino Acid Residues ◽  
Counting System ◽  
Single Photon Counting ◽  
Decay Behaviour ◽  
The Individual

The state of three tryptophyl residues in human serum orosomucoid was estimated by prediction methods based on parameters characterizing their hydrophobicity either directly, or in terms of buried surfaces of the individual amino acid residues. It is shown that tryptophan 25 is the most buried, while Trp 160 is the most exposed to the solvent. Trp 122 is in this respect in an intermediate state. The fluorescence decay behaviour was determined using a picosecond single photon counting system. The multiwavelength data were analyzed using a global analysis as well as a distribution of lifetimes program. Both procedures yielded the existence of four wavelength independent lifetimes (0.22 ns, 1.0 ns, 2.5 ns, and 8.4 ns). A tentative assignment of the decay associated spectra of the four components to the three individual tryptophans is presented.

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