Improved fibrin plate method for fibrinolytic activity measurements: Use of bentonite precipitation and agar solidification

1975 ◽  
Vol 60 (1) ◽  
pp. 85-89 ◽  
Author(s):  
Lynn Deogny ◽  
Annita Weidenbach ◽  
James W. Hampton
Keyword(s):  
Fibrinolytic Activity ◽  
Plate Method ◽  
Fibrin Plate ◽  
Activity Measurements

Fibrinolytic Activity of Cerebro-Spinal Fluid and the Development of Artificial Cerebral Haematomas in Dogs

1969 ◽  
Vol 21 (02) ◽  
pp. 294-303 ◽  
Author(s):  
H Mihara ◽  
T Fujii ◽  
S Okamoto
Keyword(s):  
Cerebrospinal Fluid ◽  
Carboxylic Acid ◽  
Fibrinolytic Activity ◽  
Clinical Implications ◽  
Trans Form ◽  
Plate Method ◽  
Blood Samples ◽  
Fibrin Plate ◽  
The Brain

SummaryBlood was injected into the brains of dogs to produce artificial haematomas, and paraffin injected to produce intracerebral paraffin masses. Cerebrospinal fluid (CSF) and peripheral blood samples were withdrawn at regular intervals and their fibrinolytic activities estimated by the fibrin plate method. Trans-form aminomethylcyclohexane-carboxylic acid (t-AMCHA) was administered to some individuals. Genera] relationships were found between changes in CSF fibrinolytic activity, area of tissue damage and survival time. t-AMCHA was clearly beneficial to those animals given a programme of administration. Tissue activator was extracted from the brain tissue after death or sacrifice for haematoma examination. The possible role of tissue activator in relation to haematoma development, and clinical implications of the results, are discussed.

GRANULOCYTE ELASTASE RELEASE DURING BLOOD COAGULATION

1987 ◽  
Author(s):  
T Miura ◽  
M Inagaki ◽  
M Taki ◽  
N Saito ◽  
T Meguro ◽  
...  
Keyword(s):  
Blood Coagulation ◽  
Fibrinolytic Activity ◽  
Coagulation System ◽  
Factor Xii ◽  
High Potency ◽  
Plate Method ◽  
Granulocyte Elastase ◽  
A 23187 ◽  
Fibrin Plate ◽  
Dose Dependent

Granulocyte elastase (ELP) has a high-potency fibrinolytic activity. Hence, there is a possibility that ELP acts as a thrombolytic enzyme like plasmin in thrombolysis. We investigated the release of ELP from granulocytes, especially during blood coagulation.The biological activity of ELP was measured using a synthetic substrate, Suc-Ala-Tyr-Leu-Val-pNA. The immunological activity assayed as an alpha-l-antitrypsin-ELP complex was measured using an anti-ELP antibody (Merck), because more than 90% of ELP in blood forms alpha-l-antitrypsin-ELP complexes.The ELP activity in granulocytes extracted by 2 mol/1 KSCN was 10 mU/106 cells. This fibrinolytic activity corresponds to 1-2 U of plasmin in the fibrin plate method.The ELP release from separated granulocytes was observed by adding Ca2+, and the release was increased by Ca ionophore A 23187. The release was dose-dependent as far as 10 mM Ca2+ (final concentration) and the maximum release was obtained within 15 minutes. However, the ELP release was not produced by thrombin.The level of alpha-l-antitrypsin-ELP complex in serum was twice higher and that in heparinized plasma was 1.5 times higher than that in sodium citrated plasma. ELP was not released from granulocytes incubated in both prekallikrein deficient plasma and Factor XII deficient plasma containing 10 mM Ca2+. But addition of normal plasma (about 10%) resulted in ELP releaseThese results suggest that the ELP release from granulocytes is dependent on Ca2+ and the release is relevant to the blood coagulation system, especially to contact factors.

Comparative Research on the Fibrinolytic Action of Different Nicotinic Acid Derivatives Following Their Prolonged Administration into Rabbits

1966 ◽  
Vol 15 (01/02) ◽  
pp. 231-237 ◽  
Author(s):  
P de Nicola ◽  
A Gibelli ◽  
G Turazza
Keyword(s):  
Nicotinic Acid ◽  
Comparative Research ◽  
Fibrinolytic Activity ◽  
Prolonged Administration ◽  
Plate Method ◽  
Fibrin Plate ◽  
Nicotinic Acid Derivatives ◽  
Heated Plates ◽  
Fibrinolytic Action

SummarySix different nicotinic acid derivatives were given intravenously into rabbits for 28 days. The dosage was calculated on the basis of equivalents of nicotinic acid. Fibrinolytic activity in plasma was studied by means of the fibrin plate method (heated plates; euglobulinic precipitate, with and without the addition of activator). Plasmin and plasminogen activities were evaluated. The most significant results concern the differences in duration, intensity and type (plasmin and/or plasminogen increase) of effect of the different nicotinic acid derivatives.

scholarly journals Fibrinolytic Activity of Hydatidiform Molar Tissue

1977 ◽  
Author(s):  
F. H. M. Tsakok ◽  
S. S. Ratnam
Keyword(s):  
Fibrinolytic Activity ◽  
Hydatidiform Mole ◽  
Placental Tissue ◽  
Uterine Bleeding ◽  
Fibrinolytic System ◽  
Coagulation System ◽  
Molar Pregnancy ◽  
Plate Method ◽  
Fibrin Plate ◽  
Normal Placenta

Intravascular coagulation has been previously suspected in hydatidiform molar pregnancy (McKay 1965) and isolated cases have been described (Egley 1974). Recently a comprehensive coagulation profile in 18 intact hydatidiform molar pregnancies has been reported (Tsakok 1976). These studies showed evidence of abnormal proteolysis with activation of the coagulation system and the fibrinolytic system in varying degrees. In the present work the fibrinolytic activity of fresh molar tissue from 16 patients was studied by the fibrin plate method (Nilsson 1962) and by histochemistry (Pandolfi 1972). The fibrinolytic activity was compared with fresh normal placental tissue.Marked fibrinolytic activity was found in the hydatidiform molar tissue as compared to none in the normal placenta. Increased fibrinolytic activity of hydatidiform mole may be the cause of early, prolonged and heavy uterine bleeding in such abnormal pregnancies. This may be due to local activation of the fibrinolytic system.

scholarly journals Brief Report: Protease Content and Fibrinolytic Activity of Human Leukocytes

Blood ◽  
1967 ◽  
Vol 29 (1) ◽  
pp. 134-138 ◽  
Author(s):  
TAGE ASTRUP ◽  
JØRGEN HENRICHSEN ◽  
HAU C. KWAAN
Keyword(s):  
Endothelial Cells ◽  
Plasminogen Activator ◽  
Fibrinolytic Activity ◽  
The Body ◽  
Plate Method ◽  
Human Leukocytes ◽  
Fibrin Plate ◽  
Capillary Endothelial Cells

Abstract The fibrinolytic activity of human leukocytes was studied by the fibrin plate method and by the histochemical fibrin slide method, using plasminogen-rich and plasminogen-free fibrin substrates. Lysis is caused by a protease. Plasminogen activator is absent. The slide method showed the effect of leukocytes to be weak in comparison to that produced by the plasminogen activator in capillary endothelial cells invading fibrin deposits in the body.

A New Method Based on One Dimensional Diffusion Using Small Glass Tubes for the Measurement of Fibrinolytic Activity

1972 ◽  
Vol 28 (03) ◽  
pp. 329-341 ◽  
Author(s):  
Taro Yasukouchi ◽  
Takeo Watanabe
Keyword(s):  
Fibrinolytic Activity ◽  
New Method ◽  
Internal Diameter ◽  
Plate Method ◽  
Simple Diffusion ◽  
One Dimensional ◽  
Dimensional Diffusion ◽  
Small Glass ◽  
Fibrin Plate ◽  
Glass Tubes

SummaryA new method of estimating fibrinolytic activity is introduced. This method based on one dimensional diffusion (simple diffusion) is characterized by adopting small glass tubes, internal diameter about 3.5 mm calibrated from the bottom by 50 mm, as containers of substratecl fibrin.The results and advantages over previous methods are as follows :1. Clear line of demarcation between lyzed zone and remaining fibrin makes it easier to measure the extract of lysis than in the fibrin plate method.2. The enzyme activity of materials tested is kept stable for many hours.3. The quantity of test material in the fibrin tube is optional.4. Only 1/10 fibrinogen required for fibrin plate method is sufficient.5. Adopting a standard euglobulin unit gives a free choice of experimental conditions. The pooled euglobulin prepared from 48 healthy persons represented 1 standard euglobulin unit.Standard deviations of “Euglobulin + Streptokinase” level in plasmas from 24 healthy men (aged 18-54 yrs) and women (aged 17-50 yrs) were ±0.27 and ±0.20 standard euglobulin units, respectively.

Fibrinolytic Activity in Man During Surgery

1962 ◽  
Vol 07 (03) ◽  
pp. 391-403 ◽  
Author(s):  
Lennart Andersson ◽  
Inga Marie Nilsson ◽  
Bertil Olow
Keyword(s):  
Blood Loss ◽  
Plasminogen Activator ◽  
Fibrinolytic Activity ◽  
Aminocaproic Acid ◽  
Coagulation Disorder ◽  
Normal Reaction ◽  
Plate Method ◽  
Fibrin Plate ◽  
Actual Operation ◽  
Induction Of Anaesthesia

Summary165 patients undergoing various operations were studied for any variation in the fibrinolytic activity of the blood, as measured by a modified fibrin plate method.The fibrinolytic activity was found to increase somewhat already after induction of anaesthesia, and almost always slightly to moderately during the actual operation.This activity is believed to be due to an increase of the plasminogen activator in the blood, since it can be inhibited by administration of ε-aminocaproic acid. It is presumably a normal reaction and, unless extreme or combined with some coagulation disorder, it probably has but little influence on blood loss at operation.

scholarly journals Furosemide, Fibrinolytic Activity and Urokinase Excretion

1977 ◽  
Author(s):  
I.S. Chohan ◽  
I. Singh ◽  
J. Vermylen ◽  
M. Verstraete
Keyword(s):  
Cyclic Amp ◽  
Pulmonary Oedema ◽  
Fibrinolytic Activity ◽  
Dilution Effect ◽  
Factor Xii ◽  
Plate Method ◽  
Fibrin Plate ◽  
Initial Decrease ◽  
Vasodilatory Activity ◽  
Dependent Pathway

Plasma fibrinolytic activity, as measured by the fibrin-plate method, is enhanced after an intravenous injection of 40 mg furosemide. The effect is evident within 30 minutes of the injection and attains a peak after 6 hours. If a second injection of furosemide is given at this stage, the increased fibrinolytic activity persists, and a slightly higher peak than the first is obtained again 6 hours after this injection. After the furosemide injection, there is an initial decrease of urokinase excretion which returns to normal after 3 to 6 hours.The decrease of urokinase excretion is attributed to a dilution effect during increased diuresis and the rise in fibrinolytic activity by furosemide is attributed to its vasodilatory activity and cyclic-AMP phosphodiesterase inhibitory capacity. It also appears related to the Hageman factor dependent pathway of fibrinolysis. In the treatment of high altitude pulmonary oedema, furosemide restores factor XII and fibrinolytic activity, which are both depressed in this disease (I. Singh and I.S. Chohan, Int. J. Biometeor. 18, 33, 1974).

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