scholarly journals Furosemide, Fibrinolytic Activity and Urokinase Excretion

1977 ◽  
Author(s):  
I.S. Chohan ◽  
I. Singh ◽  
J. Vermylen ◽  
M. Verstraete
Keyword(s):  
Cyclic Amp ◽  
Pulmonary Oedema ◽  
Fibrinolytic Activity ◽  
Dilution Effect ◽  
Factor Xii ◽  
Plate Method ◽  
Fibrin Plate ◽  
Initial Decrease ◽  
Vasodilatory Activity ◽  
Dependent Pathway

Plasma fibrinolytic activity, as measured by the fibrin-plate method, is enhanced after an intravenous injection of 40 mg furosemide. The effect is evident within 30 minutes of the injection and attains a peak after 6 hours. If a second injection of furosemide is given at this stage, the increased fibrinolytic activity persists, and a slightly higher peak than the first is obtained again 6 hours after this injection. After the furosemide injection, there is an initial decrease of urokinase excretion which returns to normal after 3 to 6 hours.The decrease of urokinase excretion is attributed to a dilution effect during increased diuresis and the rise in fibrinolytic activity by furosemide is attributed to its vasodilatory activity and cyclic-AMP phosphodiesterase inhibitory capacity. It also appears related to the Hageman factor dependent pathway of fibrinolysis. In the treatment of high altitude pulmonary oedema, furosemide restores factor XII and fibrinolytic activity, which are both depressed in this disease (I. Singh and I.S. Chohan, Int. J. Biometeor. 18, 33, 1974).

GRANULOCYTE ELASTASE RELEASE DURING BLOOD COAGULATION

1987 ◽  
Author(s):  
T Miura ◽  
M Inagaki ◽  
M Taki ◽  
N Saito ◽  
T Meguro ◽  
...  
Keyword(s):  
Blood Coagulation ◽  
Fibrinolytic Activity ◽  
Coagulation System ◽  
Factor Xii ◽  
High Potency ◽  
Plate Method ◽  
Granulocyte Elastase ◽  
A 23187 ◽  
Fibrin Plate ◽  
Dose Dependent

Granulocyte elastase (ELP) has a high-potency fibrinolytic activity. Hence, there is a possibility that ELP acts as a thrombolytic enzyme like plasmin in thrombolysis. We investigated the release of ELP from granulocytes, especially during blood coagulation.The biological activity of ELP was measured using a synthetic substrate, Suc-Ala-Tyr-Leu-Val-pNA. The immunological activity assayed as an alpha-l-antitrypsin-ELP complex was measured using an anti-ELP antibody (Merck), because more than 90% of ELP in blood forms alpha-l-antitrypsin-ELP complexes.The ELP activity in granulocytes extracted by 2 mol/1 KSCN was 10 mU/106 cells. This fibrinolytic activity corresponds to 1-2 U of plasmin in the fibrin plate method.The ELP release from separated granulocytes was observed by adding Ca2+, and the release was increased by Ca ionophore A 23187. The release was dose-dependent as far as 10 mM Ca2+ (final concentration) and the maximum release was obtained within 15 minutes. However, the ELP release was not produced by thrombin.The level of alpha-l-antitrypsin-ELP complex in serum was twice higher and that in heparinized plasma was 1.5 times higher than that in sodium citrated plasma. ELP was not released from granulocytes incubated in both prekallikrein deficient plasma and Factor XII deficient plasma containing 10 mM Ca2+. But addition of normal plasma (about 10%) resulted in ELP releaseThese results suggest that the ELP release from granulocytes is dependent on Ca2+ and the release is relevant to the blood coagulation system, especially to contact factors.

Fibrinolytic Activity of Cerebro-Spinal Fluid and the Development of Artificial Cerebral Haematomas in Dogs

1969 ◽  
Vol 21 (02) ◽  
pp. 294-303 ◽  
Author(s):  
H Mihara ◽  
T Fujii ◽  
S Okamoto
Keyword(s):  
Cerebrospinal Fluid ◽  
Carboxylic Acid ◽  
Fibrinolytic Activity ◽  
Clinical Implications ◽  
Trans Form ◽  
Plate Method ◽  
Blood Samples ◽  
Fibrin Plate ◽  
The Brain

SummaryBlood was injected into the brains of dogs to produce artificial haematomas, and paraffin injected to produce intracerebral paraffin masses. Cerebrospinal fluid (CSF) and peripheral blood samples were withdrawn at regular intervals and their fibrinolytic activities estimated by the fibrin plate method. Trans-form aminomethylcyclohexane-carboxylic acid (t-AMCHA) was administered to some individuals. Genera] relationships were found between changes in CSF fibrinolytic activity, area of tissue damage and survival time. t-AMCHA was clearly beneficial to those animals given a programme of administration. Tissue activator was extracted from the brain tissue after death or sacrifice for haematoma examination. The possible role of tissue activator in relation to haematoma development, and clinical implications of the results, are discussed.

HOMOZYGOUS FACTOR XII CONGENITAL DEFICIENCY: STUDY OF 10 NEW FAMILIES.

1987 ◽  
Author(s):  
G Castaman ◽  
F Rodeghiero ◽  
M Ruggeri
Keyword(s):  
Fibrinolytic Activity ◽  
Normal Values ◽  
Common Finding ◽  
Factor Xii ◽  
Normal Range ◽  
Congenital Deficiency ◽  
Fibrin Plate ◽  
Sporadic Cases ◽  
Laboratory Investigations ◽  
Normal Controls

Sporadic cases of thromboembolic events have been reported in patients with congenital factor XII deficiency and a relationship with a reduced intrinsic fibrinolysis has been suggested.We report here the results of clinical and laboratory investigations in 10 new families comprising 15 homozygotes (age 16-72) and 14 heterozygotes (age 18-65).In homozygotes, kaolin-activated-PTT was indefinitely prolonged and F XII activity and antigen were undetectable, whereas functional assays . of high molecular weight kininogen ahd kallikrein yielded normal values. Intrinsic fibrinolytic activity - assayed on fibrin plate by measuring lysis zones determined i. by euglobulin fraction, obtained in presence of dextran sulphate and flufenamate (Blood activator inventory test, Kluft 1979) - was reduced in all homozygous pts. to about 50% of normal (range 15-70%; normal range 80-120%); normal values were observed in all heterozygotes. Basal extrinsic fibrinolytic activity (measured after addition of Cl-inhibitor) was absent or minimal as in normal controls. None of our patients showed evidence of thrombotic diathesis.In conclusion, our study demonstrates that a reduced intrinsic fibrinolysis, as assayed by blood activator inventory test, is a common finding in F XII deficiency. The absence of thrombotic diathesis in our cases suggests that, this defect is probably devoid of any clinical significance.

Comparative Research on the Fibrinolytic Action of Different Nicotinic Acid Derivatives Following Their Prolonged Administration into Rabbits

1966 ◽  
Vol 15 (01/02) ◽  
pp. 231-237 ◽  
Author(s):  
P de Nicola ◽  
A Gibelli ◽  
G Turazza
Keyword(s):  
Nicotinic Acid ◽  
Comparative Research ◽  
Fibrinolytic Activity ◽  
Prolonged Administration ◽  
Plate Method ◽  
Fibrin Plate ◽  
Nicotinic Acid Derivatives ◽  
Heated Plates ◽  
Fibrinolytic Action

SummarySix different nicotinic acid derivatives were given intravenously into rabbits for 28 days. The dosage was calculated on the basis of equivalents of nicotinic acid. Fibrinolytic activity in plasma was studied by means of the fibrin plate method (heated plates; euglobulinic precipitate, with and without the addition of activator). Plasmin and plasminogen activities were evaluated. The most significant results concern the differences in duration, intensity and type (plasmin and/or plasminogen increase) of effect of the different nicotinic acid derivatives.

The Role of HF (Factor XII) in the Pathogenesis of the Thrombolytic State Induced by Venous Occlusion

1966 ◽  
Vol 16 (01/02) ◽  
pp. 207-217 ◽  
Author(s):  
S. G Iatridis ◽  
P. G Iatridis ◽  
J. H Ferguson
Keyword(s):  
Normal Subjects ◽  
Venous Occlusion ◽  
Inhibitory Effect ◽  
Lytic Activity ◽  
Factor Xii ◽  
Plate Method ◽  
Lysis Time ◽  
Low Concentrations ◽  
Fibrin Plate ◽  
Euglobulin Lysis Time

SummaryThe effect of venous occlusion of the forearm on clotting and fibrinolysis was studied on 17 normal subjects. A post-occlusion increase in AHF (factor (VIII) and HF (factor XII) was noted. Fibrinolytic activity, as measured by euglobulin lysis time, plasma plate method, unheated fibrin plate method and plasminoplastin generation test, increased significantly in most of the subjects. A plasma lysokinase (indirect activator) seems to preponderate; this was also accompanied by some plasminoplastin (direct activator) activity. Low concentrations of ε-ACA and ATPase had an inhibitory effect upon the increased lytic activity. The data suggest that the post-occlusion lytic activity which was characterized to be of the lysokinase-type was intimately related to HF (factor XII).

scholarly journals Fibrinolytic Activity of Hydatidiform Molar Tissue

1977 ◽  
Author(s):  
F. H. M. Tsakok ◽  
S. S. Ratnam
Keyword(s):  
Fibrinolytic Activity ◽  
Hydatidiform Mole ◽  
Placental Tissue ◽  
Uterine Bleeding ◽  
Fibrinolytic System ◽  
Coagulation System ◽  
Molar Pregnancy ◽  
Plate Method ◽  
Fibrin Plate ◽  
Normal Placenta

Intravascular coagulation has been previously suspected in hydatidiform molar pregnancy (McKay 1965) and isolated cases have been described (Egley 1974). Recently a comprehensive coagulation profile in 18 intact hydatidiform molar pregnancies has been reported (Tsakok 1976). These studies showed evidence of abnormal proteolysis with activation of the coagulation system and the fibrinolytic system in varying degrees. In the present work the fibrinolytic activity of fresh molar tissue from 16 patients was studied by the fibrin plate method (Nilsson 1962) and by histochemistry (Pandolfi 1972). The fibrinolytic activity was compared with fresh normal placental tissue.Marked fibrinolytic activity was found in the hydatidiform molar tissue as compared to none in the normal placenta. Increased fibrinolytic activity of hydatidiform mole may be the cause of early, prolonged and heavy uterine bleeding in such abnormal pregnancies. This may be due to local activation of the fibrinolytic system.

scholarly journals Brief Report: Protease Content and Fibrinolytic Activity of Human Leukocytes

Blood ◽  
1967 ◽  
Vol 29 (1) ◽  
pp. 134-138 ◽  
Author(s):  
TAGE ASTRUP ◽  
JØRGEN HENRICHSEN ◽  
HAU C. KWAAN
Keyword(s):  
Endothelial Cells ◽  
Plasminogen Activator ◽  
Fibrinolytic Activity ◽  
The Body ◽  
Plate Method ◽  
Human Leukocytes ◽  
Fibrin Plate ◽  
Capillary Endothelial Cells

Abstract The fibrinolytic activity of human leukocytes was studied by the fibrin plate method and by the histochemical fibrin slide method, using plasminogen-rich and plasminogen-free fibrin substrates. Lysis is caused by a protease. Plasminogen activator is absent. The slide method showed the effect of leukocytes to be weak in comparison to that produced by the plasminogen activator in capillary endothelial cells invading fibrin deposits in the body.

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