Alterations in protein, sialic acid and some enzymes in cervical mucus of female rats during NET-EN treatment

Contraception ◽  
1987 ◽  
Vol 36 (2) ◽  
pp. 227-237 ◽  
Author(s):  
T. Bhowmik ◽  
M. Mukherjea
Keyword(s):  
Sialic Acid ◽  
Cervical Mucus ◽  
Female Rats

ISOLEMENT ET QUELQUES CARACTERISTIQUES D'UNE GONADOTROPHINE URINAIRE DE MÉNOPAUSE HOMOGÈNE A L'ELECTROPHORÈSE

1960 ◽  
Vol XXXV (II) ◽  
pp. 225-234 ◽  
Author(s):  
R. Bourrillon ◽  
R. Got ◽  
R. Marcy
Keyword(s):  
Sialic Acid ◽  
Histological Study ◽  
Active Material ◽  
Biologically Active ◽  
New Method ◽  
Female Rats ◽  
Mouse Uterus ◽  
Highly Active ◽  
Zone Electrophoresis ◽  
Human Menopausal Gonadotrophin

ABSTRACT A new method for preparation of Human Menopausal Gonadotrophin involves successively alcoholic precipitation, kaolin adsorption and chromatography on ion exchangers. A highly active material is obtained which corresponds to 1 mg per litre of urine and has an activity of 1 mouse uterus unit at a dose of 0.003 mg. This gonadotrophin possesses both follicle stimulating and luteinizing activities in hypophysectomized female rats, by histological study. It contains 13 % hexose, 10% hexosamine and 8.5 % sialic acid. A further purification, by zone electrophoresis on starch, gives a final product, biologically active at 0.001 mg, which behaves as an homogenous substance in free electrophoresis with mobility −4.76 × 10−5 at pH 8.6.

Characterisation of sialic acid species in sheep cervical mucus

2020 ◽  
Vol 220 ◽  
pp. 106361
Author(s):  
Laura Abril-Parreño ◽  
Jack Morgan ◽  
Anette Krogenæs ◽  
Xavier Druart ◽  
Mary E. Gallagher ◽  
...  
Keyword(s):  
Sialic Acid ◽  
Cervical Mucus

scholarly journals The Detection of Bovine Estrus by Lactoferrin Monoclonal Antibody

Animals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1582
Author(s):  
Jihwan Lee ◽  
Suhyun Lee ◽  
Younbae Park ◽  
Seokhyun Lee ◽  
Seungmin Ha ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Binding Affinity ◽  
Reproductive Performance ◽  
Economic Loss ◽  
Cervical Mucus ◽  
Female Rats ◽  
Economic Damage ◽  
Bovine Lactoferrin ◽  
Estrous Synchronization ◽  
High Binding Affinity

To improve reproductive performance in cattle, the accurate detection of estrus and optimization of insemination relative to ovulation are necessary. However, poor heat detection by farm staff leads to a decreased conception rate, thus inflicting economic damage to the beef and dairy industries. This study aimed to develop monoclonal antibodies (mAb) that can specifically bind to the bovine lactoferrin (bLF) protein, which we have previously demonstrated to be overexpressed in bovine cervical mucus during estrus. Female rats were intraperitoneally immunized with bLF protein as the antigen. Anti-bLF mAbs were then purified by affinity chromatography, and their binding affinity for the bLF antigen was examined using ELISA. We found a high binding affinity between mAbs and bLF. Finally, we developed a rapid bovine heat detection kit using the anti-bLF mAbs that we generated and tested on cervical mucus from 12 cows (estrous synchronization, n = 2; natural cycling, n = 10). We found that the kits accurately detected estrus. Overall, our fabricated heat detection kit based on rat anti-bLF mAbs could pave the way for the development of potent tools for heat detection devices for dairy cattle, thereby preventing economic loss.

SIALIC ACID CONTENT AND SPERM RECEPTIVITY OF CERVICAL MUCUS IN RELATION TO OESTROGEN EXCRETION FOLLOWING ADMINISTRATION OF FSH

1969 ◽  
Vol 62 (4) ◽  
pp. 711-720 ◽  
Author(s):  
L. Carlborg ◽  
C. Gemzell
Keyword(s):  
Sialic Acid ◽  
Acid Content ◽  
Cervical Mucus ◽  
Human Pituitary ◽  
Sialic Acid Content ◽  
Oestrogen Level

ABSTRACT The cervical mucus was studied with regard to changes in sialic acid content and sperm receptivity. The data were correlated with the urinary total oestrogen excretion in anovulatory women treated with human pituitary gonadotrophins (HPG). With increasing total oestrogen excretion the sialic acid content decreased and the sperm receptivity increased. At a total oestrogen level of about 60 μg/24 h the respective patterns of the curves were reversed and higher values for total oestrogen were actually associated with a decrease in sperm receptivity and an increase in sialic acid content. The possible significance of these observations is discussed.

scholarly journals Cervical mucus sialic acid content determines the ability of frozen-thawed ram sperm to migrate through the cervix

Reproduction ◽  
2019 ◽  
Vol 157 (3) ◽  
pp. 259-271 ◽  
Author(s):  
L Richardson ◽  
J P Hanrahan ◽  
T Tharmalingam ◽  
S D Carrington ◽  
P Lonergan ◽  
...  
Keyword(s):  
Sialic Acid ◽  
Acid Content ◽  
Cervical Mucus ◽  
Sialic Acid Content ◽  
Sperm Transport ◽  
Glycosidase Activity ◽  
Sperm Penetration ◽  
Muc Genes ◽  
The Difference ◽  
Ram Sperm

The aim of this study was to investigate the properties and to functionally characterize the cervical mucus that modulates sperm transport through the cervix by using ewe breeds with a divergent pregnancy rate (Belclare and Suffolk; high and low, respectively) following cervical insemination using frozen-thawed semen. Sperm number, as well as sialic acid and fucose content in both the channels and in the lumen of different regions of the cervix were quantified in inseminated Belclare and Suffolk ewes. Expression of glycosyltransferase and MUC genes, glycosidase activity and sialic acid speciation in follicular phase cervical tissue and mucus were assessed. More spermatozoa were found in the cervical channels in the region closest to the cervical os in Belclare than Suffolk ewes (P < 0.05) and Suffolk ewes had a higher sialic acid content in the cervical channels than Belclare ewes (P < 0.05) in all regions of cervix. Suffolk ewes had significantly higher expression of FUT1, ST6GAL1 and MUC5AC than Belclare ewes. There was no difference between the breeds in glycosidase activity (P > 0.05). Levels of Neu5Ac were higher in Belclare than Suffolk ewes (P < 0.05) and levels of Neu5Gc was higher in Suffolk than Belclare ewes (P < 0.05). Competitive sperm penetration assays demonstrated that frozen-thawed sperm progression increased when cervical mucus was incubated with sialyllactose prior to a sperm penetration test (P < 0.05). These results suggest that the difference between Belclare and Suffolk ewes in sperm transport with frozen-thawed semen is due to the higher concentration of sialic acid within channels, which binds to spermatozoa and reduces their ability to traverse the cervix.

SIALIC ACID CONTENT AND SPERM PENETRATION OF CERVICAL MUCUS IN RELATION TO TOTAL URINARY OESTROGEN EXCRETION AND PLASMA PROGESTERONE LEVELS IN OVULATORY WOMEN

1969 ◽  
Vol 62 (4) ◽  
pp. 721-731 ◽  
Author(s):  
Lars Carlborg ◽  
Elof D. B. Johansson ◽  
Carl Gemzell
Keyword(s):  
Sialic Acid ◽  
Acid Content ◽  
Cervical Mucus ◽  
Ovulatory Cycle ◽  
Sialic Acid Content ◽  
Plasma Progesterone ◽  
Sperm Penetration ◽  
Menstruating Women

ABSTRACT Daily measurements of sialic acid content and sperm penetration into the cervical mucus were related to the total urinary oestrogen excretion and plasma progesterone levels during one ovulatory cycle in six regularly menstruating women. In four women of known fertility and in one normally menstruating woman the peak in sperm penetration and the dip in sialic acid content occurred almost simultaneously with the ovulatory oestrogen peak in urine and preceded the probable day of ovulation by one day. In one woman who was barren for several years the steroid patterns were normal but the peak in sperm penetration and the dip in sialic acid content occurred one day after ovulation. The possible significance of this observation and other data are discussed.

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