SIALIC ACID CONTENT AND SPERM PENETRATION OF CERVICAL MUCUS IN RELATION TO TOTAL URINARY OESTROGEN EXCRETION AND PLASMA PROGESTERONE LEVELS IN OVULATORY WOMEN

1969 ◽  
Vol 62 (4) ◽  
pp. 721-731 ◽  
Author(s):  
Lars Carlborg ◽  
Elof D. B. Johansson ◽  
Carl Gemzell
Keyword(s):  
Sialic Acid ◽  
Acid Content ◽  
Cervical Mucus ◽  
Ovulatory Cycle ◽  
Sialic Acid Content ◽  
Plasma Progesterone ◽  
Sperm Penetration ◽  
Menstruating Women

ABSTRACT Daily measurements of sialic acid content and sperm penetration into the cervical mucus were related to the total urinary oestrogen excretion and plasma progesterone levels during one ovulatory cycle in six regularly menstruating women. In four women of known fertility and in one normally menstruating woman the peak in sperm penetration and the dip in sialic acid content occurred almost simultaneously with the ovulatory oestrogen peak in urine and preceded the probable day of ovulation by one day. In one woman who was barren for several years the steroid patterns were normal but the peak in sperm penetration and the dip in sialic acid content occurred one day after ovulation. The possible significance of this observation and other data are discussed.

scholarly journals Cervical mucus sialic acid content determines the ability of frozen-thawed ram sperm to migrate through the cervix

Reproduction ◽  
2019 ◽  
Vol 157 (3) ◽  
pp. 259-271 ◽  
Author(s):  
L Richardson ◽  
J P Hanrahan ◽  
T Tharmalingam ◽  
S D Carrington ◽  
P Lonergan ◽  
...  
Keyword(s):  
Sialic Acid ◽  
Acid Content ◽  
Cervical Mucus ◽  
Sialic Acid Content ◽  
Sperm Transport ◽  
Glycosidase Activity ◽  
Sperm Penetration ◽  
Muc Genes ◽  
The Difference ◽  
Ram Sperm

The aim of this study was to investigate the properties and to functionally characterize the cervical mucus that modulates sperm transport through the cervix by using ewe breeds with a divergent pregnancy rate (Belclare and Suffolk; high and low, respectively) following cervical insemination using frozen-thawed semen. Sperm number, as well as sialic acid and fucose content in both the channels and in the lumen of different regions of the cervix were quantified in inseminated Belclare and Suffolk ewes. Expression of glycosyltransferase and MUC genes, glycosidase activity and sialic acid speciation in follicular phase cervical tissue and mucus were assessed. More spermatozoa were found in the cervical channels in the region closest to the cervical os in Belclare than Suffolk ewes (P < 0.05) and Suffolk ewes had a higher sialic acid content in the cervical channels than Belclare ewes (P < 0.05) in all regions of cervix. Suffolk ewes had significantly higher expression of FUT1, ST6GAL1 and MUC5AC than Belclare ewes. There was no difference between the breeds in glycosidase activity (P > 0.05). Levels of Neu5Ac were higher in Belclare than Suffolk ewes (P < 0.05) and levels of Neu5Gc was higher in Suffolk than Belclare ewes (P < 0.05). Competitive sperm penetration assays demonstrated that frozen-thawed sperm progression increased when cervical mucus was incubated with sialyllactose prior to a sperm penetration test (P < 0.05). These results suggest that the difference between Belclare and Suffolk ewes in sperm transport with frozen-thawed semen is due to the higher concentration of sialic acid within channels, which binds to spermatozoa and reduces their ability to traverse the cervix.

SIALIC ACID CONTENT AND SPERM RECEPTIVITY OF CERVICAL MUCUS IN RELATION TO OESTROGEN EXCRETION FOLLOWING ADMINISTRATION OF FSH

1969 ◽  
Vol 62 (4) ◽  
pp. 711-720 ◽  
Author(s):  
L. Carlborg ◽  
C. Gemzell
Keyword(s):  
Sialic Acid ◽  
Acid Content ◽  
Cervical Mucus ◽  
Human Pituitary ◽  
Sialic Acid Content ◽  
Oestrogen Level

ABSTRACT The cervical mucus was studied with regard to changes in sialic acid content and sperm receptivity. The data were correlated with the urinary total oestrogen excretion in anovulatory women treated with human pituitary gonadotrophins (HPG). With increasing total oestrogen excretion the sialic acid content decreased and the sperm receptivity increased. At a total oestrogen level of about 60 μg/24 h the respective patterns of the curves were reversed and higher values for total oestrogen were actually associated with a decrease in sperm receptivity and an increase in sialic acid content. The possible significance of these observations is discussed.

129: Sialic Acid Content of Urinary TAMM-Horsfall Protein is Reduced in Interstitial Cystitis Patients

2007 ◽  
Vol 177 (4S) ◽  
pp. 44-45
Author(s):  
C. Lowell Parsons ◽  
Mahadevan Rajasekaran ◽  
Marianne Chenoweth ◽  
Paul Stein
Keyword(s):  
Sialic Acid ◽  
Interstitial Cystitis ◽  
Acid Content ◽  
Sialic Acid Content

scholarly journals Differences in Sialic Acid Content of Human Interferons

1978 ◽  
Vol 41 (1) ◽  
pp. 175-178 ◽  
Author(s):  
J. Morser ◽  
J. P. Kabayo ◽  
D. W. Hutchinson
Keyword(s):  
Sialic Acid ◽  
Acid Content ◽  
Sialic Acid Content

scholarly journals Infection with Listeria monocytogenes impairs sialic acid addition to host cell glycoproteins.

1994 ◽  
Vol 180 (6) ◽  
pp. 2137-2145 ◽  
Author(s):  
M S Villanueva ◽  
C J Beckers ◽  
E G Pamer
Keyword(s):  
Listeria Monocytogenes ◽  
Sialic Acid ◽  
Host Cell ◽  
Mhc Class I ◽  
Acid Content ◽  
Class I ◽  
Sialic Acid Content ◽  
Infected Cells ◽  
The Impact ◽  
Glycosylation Defect

Listeria monocytogenes is a facultative intracellular bacterium that causes severe disease in neonates and immunocompromised adults. Although entry, multiplication, and locomotion of Listeria in the cytosol of infected cells are well described, the impact of such infection on the host cell is unknown. In this report, we investigate the effect of L. monocytogenes infection on MHC class I synthesis, processing, and intracellular trafficking. We show that L. monocytogenes infection interferes with normal processing of N-linked oligosaccharides on the major histocompatibility complex (MHC) class I heavy chain molecule, H-2Kd, resulting in a reduced sialic acid content. The glycosylation defect is more pronounced as the infection progresses and results from interference with the addition of sialic acid rather than its removal by a neuraminidase. The effect is found in two different cell lines and is not limited to MHC class I molecules since CD45, a surface glycoprotein, and LGP120, a lysosomal glycoprotein, are similarly affected by L. monocytogenes infection. The glycosylation defect is specific for infection by L. monocytogenes since neither Trypanosoma cruzi nor Yersinia enterocolitica, two other intracellular pathogens, reproduces the effect. The resultant hyposialylation of H-2Kd does not impair its surface expression in infected cells. Diminished sialic acid content of surface glycoproteins may enhance host-defense by increasing susceptibility to lysis and promoting clearance of Listeria-infected cells.

scholarly journals Erythrocyte Sialic Acid Content during Aging in Humans: Correlation with Markers of Oxidative Stress

2012 ◽  
Vol 32 (3) ◽  
pp. 179-186 ◽  
Author(s):  
Mohammad Murtaza Mehdi ◽  
Prabhakar Singh ◽  
Syed Ibrahim Rizvi
Keyword(s):  
Oxidative Stress ◽  
Sialic Acid ◽  
Erythrocyte Membrane ◽  
Acid Content ◽  
Lipid Hydroperoxide ◽  
Plasma Lipid ◽  
Sialic Acids ◽  
Sialic Acid Content ◽  
Total Antioxidant ◽  
The Relationship

Sialic acids are substituted neuraminic acid derivatives which are typically found at the outermost end of glycan chains on the membrane in all cell types. The role of erythrocyte membrane sialic acids during aging has been established however the relationship between sialic acid and oxidative stress is not fully understood. The present work was undertaken to analyze the relationship between erythrocyte membrane sialic acid with its plasma level, membrane and plasma lipid hydroperoxide levels and plasma total antioxidant capacity. Results show that sialic acid content decreases significantly (P< 0.001) in RBC membrane (r= −0.901) and increases in plasma (r= 0.860) as a function of age in humans. Lipid peroxidation measured in the form of hydroperoxides increases significantly (P< 0.001) in plasma (r= 0.830) and RBC membranes (r= 0.875) with age in humans. The Trolox Equivalent Total Antioxidant Capacity (TETAC) of plasma was found to be significantly decreased (P< 0.001,r= −0.844). We observe significant correlations between decrease of erythrocyte membrane sialic acid and plasma lipid hydroperoxide and TETAC. Based on the observed correlations, we hypothesize that increase in oxidative stress during aging may influence the sialic acid decomposition from membrane thereby altering the membrane configuration affecting many enzymatic and transporter activities. Considering the importance of plasma sialic acid as a diagnostic parameter, it is important to establish age-dependent reference.

Tamm-Horsfall glycoprotein: role in inhibition and promotion of renal calcium oxalate stone formation studied with Fourier-transform infrared spectroscopy

1994 ◽  
Vol 40 (9) ◽  
pp. 1739-1743 ◽  
Author(s):  
R Knörle ◽  
P Schnierle ◽  
A Koch ◽  
N P Buchholz ◽  
F Hering ◽  
...  
Keyword(s):  
Fourier Transform ◽  
Infrared Spectroscopy ◽  
Sialic Acid ◽  
Calcium Oxalate ◽  
Fourier Transform Infrared Spectroscopy ◽  
Acid Content ◽  
Stone Formation ◽  
Fourier Transform Infrared ◽  
Sialic Acid Content ◽  
Stone Formers

Abstract Tamm-Horsfall glycoprotein (THP) from healthy probands inhibits the precipitation of calcium oxalate, whereas THP from individuals who repeatedly develop calcium oxalate stones has no effect or even promotes precipitation. Using Fourier-transform infrared spectroscopy, we found a structural differentiation between these functionally different THPs: a decisive difference in sialic acid content. Quantitative analysis for sialic acid showed the same results. THP from healthy probands had a high sialic acid content (51 +/- 9 g/kg), whereas THP from recurrent stone formers had a decreased sialic acid content (21 +/- 4 g/kg). This explains the dual role of THP in the precipitation of calcium oxalate and the formation of renal stones and shows the importance of glycosylation in the function of this glycoprotein.

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