scholarly journals The Detection of Bovine Estrus by Lactoferrin Monoclonal Antibody

Animals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1582
Author(s):  
Jihwan Lee ◽  
Suhyun Lee ◽  
Younbae Park ◽  
Seokhyun Lee ◽  
Seungmin Ha ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Binding Affinity ◽  
Reproductive Performance ◽  
Economic Loss ◽  
Cervical Mucus ◽  
Female Rats ◽  
Economic Damage ◽  
Bovine Lactoferrin ◽  
Estrous Synchronization ◽  
High Binding Affinity

To improve reproductive performance in cattle, the accurate detection of estrus and optimization of insemination relative to ovulation are necessary. However, poor heat detection by farm staff leads to a decreased conception rate, thus inflicting economic damage to the beef and dairy industries. This study aimed to develop monoclonal antibodies (mAb) that can specifically bind to the bovine lactoferrin (bLF) protein, which we have previously demonstrated to be overexpressed in bovine cervical mucus during estrus. Female rats were intraperitoneally immunized with bLF protein as the antigen. Anti-bLF mAbs were then purified by affinity chromatography, and their binding affinity for the bLF antigen was examined using ELISA. We found a high binding affinity between mAbs and bLF. Finally, we developed a rapid bovine heat detection kit using the anti-bLF mAbs that we generated and tested on cervical mucus from 12 cows (estrous synchronization, n = 2; natural cycling, n = 10). We found that the kits accurately detected estrus. Overall, our fabricated heat detection kit based on rat anti-bLF mAbs could pave the way for the development of potent tools for heat detection devices for dairy cattle, thereby preventing economic loss.

Antitumor effects of a novel monoclonal antibody with high binding affinity to ganglioside GD3

1993 ◽  
Vol 36 (4) ◽  
pp. 260-266 ◽  
Author(s):  
So Ohta ◽  
Ayumi Honda ◽  
Yuko Tokutake ◽  
Hajime Yoshida ◽  
Nobuo Hanai
Keyword(s):  
Monoclonal Antibody ◽  
Binding Affinity ◽  
Antitumor Effects ◽  
High Binding ◽  
Ganglioside Gd3 ◽  
High Binding Affinity

Preparation of chlorin e6–monoclonal antibody conjugate and its effect for photodynamic therapy

2001 ◽  
Vol 05 (05) ◽  
pp. 486-489 ◽  
Author(s):  
SHUN-ICHIRO OGURA ◽  
YUTAKA FUJITA ◽  
TOSHIAKI KAMACHI ◽  
ICHIRO OKURA
Keyword(s):  
Monoclonal Antibody ◽  
Photodynamic Therapy ◽  
Tumor Cells ◽  
Binding Affinity ◽  
Cellular Uptake ◽  
Chlorin E6 ◽  
Peptide Bonds ◽  
High Binding ◽  
Antibody Conjugate ◽  
High Binding Affinity

Chlorin e6 ( Ce 6) was conjugated with anti-tumor monoclonal antibody ( IgG 2 b ) to increase its binding affinity for tumors. The Ce 6 was activated by N -hydroxysuccinimide and directly conjugated with IgG 2 b ( IgG 2 b – Ce 6) via peptide bonds. An average of 11 Ce 6 molecules were coupled to each IgG2b molecule. IgG 2 b – Ce 6 showed high cellular uptake in tumor cells and high photocytotoxicity. These results indicate IgG 2 b – Ce 6 is a good photosensitizer with high binding affinity to tumor cells.

scholarly journals Herb-target virtual screening and network pharmacology for prediction of molecular mechanism of Danggui Beimu Kushen Wan for prostate cancer

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Hong Li ◽  
Andrew Hung ◽  
Angela Wei Hong Yang
Keyword(s):  
Prostate Cancer ◽  
Binding Affinity ◽  
Molecular Mechanisms ◽  
Biological Activities ◽  
Tight Binding ◽  
Experimental Studies ◽  
Network Pharmacology ◽  
High Morbidity ◽  
High Binding ◽  
High Binding Affinity

AbstractProstate cancer (PCa) is a cancer that occurs in the prostate with high morbidity and mortality. Danggui Beimu Kushen Wan (DBKW) is a classic formula for patients with difficult urination including PCa. This study aimed to investigate the molecular mechanisms of DBKW for PCa. We obtained DBKW compounds from our previous reviews. We identified potential targets for PCa from literature search, currently approved drugs and Open Targets database and filtered them by protein–protein interaction network analysis. We selected 26 targets to predict three cancer-related pathways. A total of 621 compounds were screened via molecular docking using PyRx and AutoDock Vina against 21 targets for PCa, producing 13041 docking results. The binding patterns and positions showed that a relatively small number of tight-binding compounds from DBKW were predicted to interact strongly and selectively with three targets. The top five high-binding-affinity compounds were selected to generate a network, indicating that compounds from all three herbs had high binding affinity against the 21 targets and may have potential biological activities with the targets. DBKW contains multi-targeting agents that could act on more than one pathway of PCa simultaneously. Further studies could focus on validating the computational results via experimental studies.

The effect of feeding endophyte infected tall fescue seed on reproductive performance in female rats

1987 ◽  
Vol 87 (1) ◽  
pp. 171-175 ◽  
Author(s):  
D.R. Varney ◽  
M. Ndefru ◽  
S.L. Jones ◽  
R. Newsome ◽  
M.R. Siegel ◽  
...  
Keyword(s):  
Reproductive Performance ◽  
Tall Fescue ◽  
Female Rats ◽  
Effect Of Feeding

scholarly journals The effect of vitamin E treatment during preovulatory period on reproductive performance of goats following estrous synchronization using intravaginal sponges

2009 ◽  
Vol 114 (1-3) ◽  
pp. 183-192 ◽  
Author(s):  
Mustafa Sönmez ◽  
Tanzer Bozkurt ◽  
Gaffari Türk ◽  
Seyfettin Gür ◽  
Meltem Kızıl ◽  
...  
Keyword(s):  
Vitamin E ◽  
Reproductive Performance ◽  
Estrous Synchronization

scholarly journals Will Climate Change Exacerbate the Economic Damage of Flood to Agricultural Production? A Case Study of Rice in Ha Tinh Province, Vietnam

2021 ◽  
Vol 9 ◽  
Author(s):  
Pham Quy Giang ◽  
Tran Trung Vy
Keyword(s):  
Climate Change ◽  
Extreme Precipitation ◽  
Agricultural Production ◽  
Climate Change Impacts ◽  
Economic Loss ◽  
Baseline Period ◽  
Rice Production ◽  
Generalized Extreme Value Distribution ◽  
Economic Damage

In developing countries in general and in Vietnam in particular, flood induced economic loss of agriculture is a serious concern since the livelihood of large populations depends on agricultural production. The objective of this study was to examine if climate change would exacerbate flood damage to agricultural production with a case study of rice production in Huong Son District of Ha Tinh Province, North-central Vietnam. The study applied a modeling approach for the prediction. Extreme precipitation and its return periods were calculated by the Generalized Extreme Value distribution method using historical daily observations and output of the MRI-CGCM3 climate model. The projected extreme precipitation data was then employed as an input of the Mike Flood model for flood modeling. Finally, an integrated approach employing flood depth and duration and crop calendar was used for the prediction of potential economic loss of rice production. Results of the study show that in comparison with the baseline period, an increase of 49.14% in the intensity of extreme precipitation was expected, while the frequency would increase 5 times by 2050s. As a result, the seriousness of floods would increase under climate change impacts as they would become more intensified, deeper and longer, and consequently the economic loss of rice production would increase significantly. While the level of peak flow was projected to rise nearly 1 m, leading the area of rice inundated to increase by 12.61%, the value of damage would rise by over 21% by 2050s compared to the baseline period. The findings of the present study are useful for long-term agricultural and infrastructural planning in order to tackle potential flooding threats to agricultural production under climate change impacts.

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