Species-specific dissociation into single cells of live sea urchin embryos by fab against membrane components of Paracentrotus lividus and Arbacia lixula

1981 ◽  
Vol 87 (2) ◽  
pp. 229-241 ◽  
Author(s):  
Hans Noll ◽  
Valeria Matranga ◽  
Piera Palma ◽  
Felicia Cutrono ◽  
Letizia Vittorelli
Keyword(s):  
Sea Urchin ◽  
Single Cells ◽  
Paracentrotus Lividus ◽  
Sea Urchin Embryos ◽  
Species Specific ◽  
Membrane Components ◽  
Arbacia Lixula

scholarly journals CHANGES IN THE POTASSIUM CONTENT OF SEA URCHIN EGGS ON FERTILIZATION

1951 ◽  
Vol 34 (3) ◽  
pp. 285-293 ◽  
Author(s):  
Anna Monroy Oddo ◽  
Maria Esposito
Keyword(s):  
Sea Urchin ◽  
Sea Water ◽  
Paracentrotus Lividus ◽  
Potassium Content ◽  
Minimum Point ◽  
Sea Urchin Eggs ◽  
Maximum Loss ◽  
Arbacia Lixula

In the eggs of Arbacia lixula and Paracentrotus lividus an uptake of K occurs during the first 10 minutes following fertilization. Between 10 and 40 minutes K is then released. Both in Arbacia and in Paracentrotus the minimum point of the curve coincides with the nuclear streak stage. A maximum loss of 25 per cent in Arbacia and 20 per cent in Paracentrotus with respect to the amount present in the unfertilized eggs has been found. From 40 minutes up to 1 hour K undergoes a further increase and when the first cleavage sets in the same amount of K is present as in the unfertilized eggs. By treating the eggs with K-free artificial sea water it has been established that about 60 per cent of the K content of the eggs is in a non-diffusible condition. Also under such conditions the eggs when fertilized are able to take up even the very small amount of K present in the medium that was released by them prior to fertilization.

scholarly journals Contrasting recruitment seasonality of sea urchin species in Gran Canaria, Canary Islands (eastern Atlantic)

2014 ◽  
Vol 15 (3) ◽  
pp. 475 ◽  
Author(s):  
S. GARCIA-SANZ ◽  
P. G. NAVARRO ◽  
F. TUYA
Keyword(s):  
Community Structure ◽  
Seasonal Variation ◽  
Sea Urchin ◽  
Sea Urchins ◽  
Late Summer ◽  
Paracentrotus Lividus ◽  
Abundant Species ◽  
Late Winter ◽  
Gran Canaria ◽  
Arbacia Lixula

Despite sea-urchins can play an important role affecting the community structure of subtidal bottoms, factors controlling the dynamics of sea-urchin populations are still poorly understood. We assessed the seasonal variation in recruitment of three sea-urchin species (Diadema africanum, Paracentrotus lividus and Arbacia lixula) at Gran Canaria Island (eastern Atlantic) via monthly deployment of artificial collectors throughout an entire annual cycle on each of four adjacent habitat patches (seagrasses, sandy patches, ‘urchin-grazed’ barrens and macroalgal-dominated beds) within a shallow coastal landscape. Paracentrotus lividus and A. lixula had exclusively one main recruitment peak in late winter-spring. Diadema africanum recruitment was also seasonal, but recruits appeared in late summer-autumn, particularly on ‘urchin-grazed’ barrens with large abundances of adult conspecifics. In conclusion, this study has demonstrated non-overlapping seasonal recruitment patterns of the less abundant species (P. lividus and A. lixula) with the most conspicuous species (D. africanum) in the study area.

scholarly journals Amino Acid Composition of Collagen Extracted from the Spicules of Sea-Urchin Embryos (Paracentrotus Lividus)

1975 ◽  
Vol 42 (2-3) ◽  
pp. 201-204 ◽  
Author(s):  
Ida Pucci-Minafra ◽  
Salvatore Minafra ◽  
Fabrizio Gianguzza ◽  
Caterina Casano
Keyword(s):  
Amino Acid ◽  
Amino Acid Composition ◽  
Acid Composition ◽  
Sea Urchin ◽  
Paracentrotus Lividus ◽  
Sea Urchin Embryos

Free choline in sea urchin embryos (Paracentrotus lividus)

1977 ◽  
Vol 33 (1) ◽  
pp. 38-39
Author(s):  
N. Wolfson ◽  
M. Acara ◽  
B. Rennick
Keyword(s):  
Sea Urchin ◽  
Paracentrotus Lividus ◽  
Sea Urchin Embryos ◽  
Free Choline

Characterization of an Alpha Type Carbonic Anhydrase from Paracentrotus lividus Sea Urchin Embryos

2016 ◽  
Vol 18 (3) ◽  
pp. 384-395 ◽  
Author(s):  
Konstantinos Karakostis ◽  
Caterina Costa ◽  
Francesca Zito ◽  
Franz Brümmer ◽  
Valeria Matranga
Keyword(s):  
Carbonic Anhydrase ◽  
Sea Urchin ◽  
Paracentrotus Lividus ◽  
Sea Urchin Embryos ◽  
Alpha Type

Developmental changes in amounts of sulfhydryl-agent decolorant released by sea urchin embryos

1974 ◽  
Vol 52 (12) ◽  
pp. 1531-1534
Author(s):  
N. Wolfson
Keyword(s):  
Sea Urchin ◽  
Paracentrotus Lividus ◽  
Tris Buffer ◽  
Developmental Changes ◽  
Cell Stage ◽  
Nitrobenzoic Acid ◽  
Sea Urchin Embryos ◽  
Saline Medium

Live embryos of the sea urchin, Paracentrotus lividus, release materials which decolor the yellow ion formed by the reagent DTNB (5,5′-dithiobis(2-nitrobenzoic acid)) in amounts titratable with dithiothreitol, and this decolorant activity increased to about the 32-cell stage, declining gradually thereafter. The embryos release decolorants into a saline medium only if it contains Tris buffer and little or no calcium and is adjusted to a pH below 8.

Autonomous expression of tissue-specific genes in dissociated sea urchin embryos

Development ◽  
1989 ◽  
Vol 107 (2) ◽  
pp. 299-307 ◽  
Author(s):  
L. Stephens ◽  
T. Kitajima ◽  
F. Wilt
Keyword(s):  
Early Development ◽  
Sea Urchin ◽  
Single Cells ◽  
Cell Types ◽  
Cell Interactions ◽  
Cell Stage ◽  
Tissue Specific ◽  
Specific Transcript ◽  
Sea Urchin Embryos ◽  
Early Stages

The effects of disrupting cell interactions in early development were investigated by examining the accumulation of a primary mesenchyme specific transcript (SM50) and an aboral ectoderm-specific transcript (Spec 1) in cultures of sea urchin embryos that were dissociated at early stages and then cultured in CFSW. The expression of both SM50 and Spec 1 is temporally correct and remains restricted to the appropriate cell types, even if the embryo is dissociated as early as the 2-cell stage and maintained as a suspension of single cells. This result is consistent with the idea that the specificity of expression of these two genes, each characteristic of different lineages, is strongly regulated by information in the egg. Average SM50 expression is half that of intact embryos, but Spec 1 expression is very low, only 10–20% of intact controls, suggesting some differences in the response of the two genes to lack of close cell interactions.

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