A rapid method for the qualitative analysis of free amino acids in animal tissues by paper chromatography

1958 ◽  
Vol 15 (1) ◽  
pp. 242-244 ◽  
Author(s):  
J.C. George ◽  
S.D. Pishawikar ◽  
K.S. Scaria
Keyword(s):  
Amino Acids ◽  
Qualitative Analysis ◽  
Rapid Method ◽  
Paper Chromatography ◽  
Free Amino Acids ◽  
Free Amino ◽  
Animal Tissues

FREE AMINO ACIDS IN THE HAEMOLYMPH OF RHODNIUS PROLIXUS STÅHL (HEMIPTERA: REDUVIIDAE)

1966 ◽  
Vol 44 (2) ◽  
pp. 241-249 ◽  
Author(s):  
Catharine Pickett ◽  
W. G. Friend
Keyword(s):  
Amino Acids ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Paper Chromatography ◽  
Free Amino Acids ◽  
Free Amino ◽  
Rhodnius Prolixus ◽  
Layer Chromatography

Nineteen free amino acids and four ninhydrin-positive compounds were detected in the haemolymph of fifth-instar nymphs of Rhodnius prolixus by the technique of thin-layer chromatography. On the sixth day after feeding, tyrosine and tryptophan appeared in the haemolymph. Their concentration increased until the 12th day when testing stopped. The concentrations of alanine, asparagine, cystine, isoleucine, phenylalanine, and threonine fluctuated moderately from day to day. The results obtained by thin-layer chromatography differed from those of a similar study in which the technique of paper chromatography was used.

Factors in Resistance of Peas to the Pea Aphid, Acyrthosiphon pisum (Harr.) (Homoptera: Aphididae). II. Amino Acids

1957 ◽  
Vol 89 (10) ◽  
pp. 457-464 ◽  
Author(s):  
J. L. Auclair ◽  
J. B. Maltais ◽  
J. J. Cartier
Keyword(s):  
Amino Acids ◽  
Paper Chromatography ◽  
Free Amino Acids ◽  
Free Amino ◽  
Acyrthosiphon Pisum ◽  
Quantitative Estimation ◽  
Preliminary Investigation ◽  
Pea Aphid ◽  
Total Amino Acid ◽  
Amino Acid Contents

In field investigations on the relative resistance of varieties of peas, Pisum sativum L., to the pea aphid, Acyrthosiphon pisum (Harr.), the average number of aphids per terminal growth for 13 years (Maltais, 1937, 1950, 1951, and unpublished technical report, 1950-54) for six varieties was as follows: Perfection, 39.6; Daisy, 32.6; Lincoln, 35.6; Laurier (H-103), 9.8; Champion of England, 11.8; and Melting Sugar, 16.8. In a preliminary investigation by Auclair and Maltais (1950), 11 free amino acids were detected in pea plant extracts by paper chromatography. From a visual comparison of chromatograms, the variety Perfection appeared to contain a higher concentration of most free amino acids than the variety Laurier. This is a report on the quantitative estimation of the free and total amino acid contents of the three susceptible varieties (Perfection, Daisy, and Lincoln) and the three resistant varieties (Laurier, Champion of England, and Melting Sugar) by the method of paper chromatography.

Evaluation of a rapid method for estimating free amino acids in silages

2002 ◽  
Vol 99 (1-4) ◽  
pp. 177-187 ◽  
Author(s):  
Ana L Winters ◽  
Janet D Lloyd ◽  
Raymond Jones ◽  
Roger J Merry
Keyword(s):  
Amino Acids ◽  
Rapid Method ◽  
Free Amino Acids ◽  
Free Amino

scholarly journals Studies of the free amino acids of Radish leaf by paper chromatography

1953 ◽  
Vol 5 (6) ◽  
pp. 220-222
Author(s):  
Tsunoyuki Saito ◽  
Toshio Watanabe
Keyword(s):  
Amino Acids ◽  
Paper Chromatography ◽  
Free Amino Acids ◽  
Free Amino

scholarly journals Free amino acids in the panicles of Dactylis glomerata in the course of their development

2015 ◽  
Vol 43 (1) ◽  
pp. 15-25
Author(s):  
T. Dąbrowska
Keyword(s):  
Amino Acids ◽  
Nitrogen Metabolism ◽  
Paper Chromatography ◽  
Free Amino Acids ◽  
Free Amino ◽  
Dactylis Glomerata ◽  
Proline Content ◽  
Proline Level

The composition of free amino acids and proline content in various parts of <i>Dactylis glomerata</i> panicles at different stages of their development were determined by paper chromatography and electrophoresis. The proline level in the spikelets was found to change widely in the course of their development. The results are discussed with reference to the role of proline in the nitrogen metabolism of plants.

scholarly journals Free amino acids of leaves and berries of Cabernet Sauvignon grapevines

OENO One ◽  
2000 ◽  
Vol 34 (1) ◽  
pp. 19
Author(s):  
Alberto Miele ◽  
Alain Carbonneau ◽  
Jacques Bouard
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Qualitative Analysis ◽  
Vitis Vinifera ◽  
Glutamic Acid ◽  
Free Amino Acids ◽  
Free Amino ◽  
Training System ◽  
Cabernet Sauvignon ◽  
Training Systems

<p style="text-align: justify;">The composition of free amino acids was studied from leaves, pericarps, skins, musts and seeds of <em>Vitis vinifera</em> L. cv. Cabernet Sauvignon. Vineyards were in the Bordeaux region and the grapevines were conducted in espalier and lyre systems. Grapes were collected at maturity and lyophilized after sampling. Extraction of free amino acids was done with a hydroalcoholic solution and their analysis was performed with an autoanalyzer. A standard of 34 amino acids was utilized for the qualitative analysis. The results showed that, for both espalier and lyre training systems, respectively, the free amino acids were predominant in the pericarps (12.85 and 11.21 mg/g dw) - 16.88 and 15.12 mg/g dw in skins and 3.29 and 2.88 g/l in musts -, followed by the seeds (2.37 and 2.32 mg/g dw) and leaves (1.87 and 1.98 mg/g dw). The most abundant free amino acids in leaves were glutamic acid (23.8 and 28.8 p. cent), aspartic acid (8.8 and 11.1 p. cent), and glutamine (10.1 and 9.4 p. cent). Proline (41.8 and 41.5 p. cent) and arginine (22.8 and 22.4 p. cent) predominated in the pericarps. In seeds, the main amino acids were proline (14.5 and 15.8 p. cent), arginine (11.0 and 11.8 p. cent), histidine (11.2 and 8.7 p. cent), and glutamic acid (11.3 and 8.2 p. cent). Grapevine training system showed some differences in the total amount and in the percentages of each free amino acid, but the pattern of these compounds for each tissue was similar for both training systems.</p>

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