FREE AMINO ACIDS IN THE HAEMOLYMPH OF RHODNIUS PROLIXUS STÅHL (HEMIPTERA: REDUVIIDAE)

1966 ◽  
Vol 44 (2) ◽  
pp. 241-249 ◽  
Author(s):  
Catharine Pickett ◽  
W. G. Friend
Keyword(s):  
Amino Acids ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Paper Chromatography ◽  
Free Amino Acids ◽  
Free Amino ◽  
Rhodnius Prolixus ◽  
Layer Chromatography

Nineteen free amino acids and four ninhydrin-positive compounds were detected in the haemolymph of fifth-instar nymphs of Rhodnius prolixus by the technique of thin-layer chromatography. On the sixth day after feeding, tyrosine and tryptophan appeared in the haemolymph. Their concentration increased until the 12th day when testing stopped. The concentrations of alanine, asparagine, cystine, isoleucine, phenylalanine, and threonine fluctuated moderately from day to day. The results obtained by thin-layer chromatography differed from those of a similar study in which the technique of paper chromatography was used.

scholarly journals ISOLATION AND HIGH-PERFORMANCE THIN-LAYER CHROMATOGRAPHY ANALYSIS OF CONDITIONAL AMINO ACIDS FROM THE FRESH LEAVES OF ALTERNANTHERA SESSILIS

2019 ◽  
pp. 137-141 ◽  
Author(s):  
SWARNAKUMARI S ◽  
Mohan S ◽  
IDA CHRISTI VE ◽  
ABINAYA S ◽  
KALAIVANI S
Keyword(s):  
Amino Acids ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Paper Chromatography ◽  
High Performance ◽  
Alternanthera Sessilis ◽  
Chromatography Analysis ◽  
Fresh Plant Material ◽  
Trial And Error Method ◽  
Layer Chromatography

Objective: The objective of the study was to isolate the conditional amino acids present in the fresh leaves extracts of Alternanthera sessilis and compare the isolated amino acids with amino acid standards. Method: The fresh plant material was used for extraction. For the extracted sample solution, paper chromatography and high-performance thin-layer chromatography (HPTLC) fingerprinting analysis were then carried out. Results: Since A. sessilis is rich in protein, various amino acids have been isolated in aggregates and paper chromatography has been performed. Amino acids were separated and identified using trial and error method. Cysteine, glycine, aspartic acid, and proline are the standard amino acids used for comparing with the isolated amino acids by HPTLC fingerprint analysis, which gives an idea for the authentication of the plant, its constituents and also provides a parameter for quality control. Conclusion: The results scientifically validate the use of A. sessilis in the traditional medicine and also as a food.

THE LESS-POLAR METABOLITES PRODUCED BY INCUBATION OF TESTOSTERONE-4-14C WITH RAT AND BOVINE BRAIN

1969 ◽  
Vol 61 (4) ◽  
pp. 641-648 ◽  
Author(s):  
Leon J. Sholiton ◽  
Emile E. Werk
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Paper Chromatography ◽  
Specific Activity ◽  
Bovine Brain ◽  
Polar Fraction ◽  
End Products ◽  
Polar Metabolites ◽  
Layer Chromatography ◽  
Constant Specific Activity

ABSTRACT Rat and bovine brain have been incubated with testosterone-4-14C under standard conditions. With use of paper chromatography, the extracted metabolites were noted to fall into less-polar, iso-polar, and more polar fractions. The components of the less-polar fraction were separated by acetylation and thin-layer chromatography and the major end-products identified by recrystallization to constant specific activity or constant 3H/14C ratios. Androst-4-enedione and 5α-dihydrotestosterone were formed consistently under the conditions utilized. Trace amounts of other less-polar metabolites were noted occasionally.

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