Stimulation of the initiation of DNA synthesis and cell division in several cultured mouse cell types

1979 ◽  
Vol 118 (2) ◽  
pp. 311-321 ◽  
Author(s):  
Minnie K. O'Farrell ◽  
Dorothie Clingan ◽  
Philip S. Rudland ◽  
Luis Jimenez De Asua
Keyword(s):  
Cell Division ◽  
Dna Synthesis ◽  
Cell Types ◽  
Mouse Cell ◽  
Stimulation Of

Stimulation of cell division and DNA replication inPrototheca richardsiby passage through larval amphibian guts

Parasitology ◽  
1993 ◽  
Vol 107 (2) ◽  
pp. 119-124 ◽  
Author(s):  
T. J. C. Beebee ◽  
A. L.-C. Wong
Keyword(s):  
Cell Division ◽  
Dna Replication ◽  
Growth Inhibition ◽  
Dna Synthesis ◽  
Free Living ◽  
Leucine Uptake ◽  
Amphibian Larvae ◽  
Larval Amphibian ◽  
Stimulation Of

SUMMARYPrototheca richardsi, an unpigmented heterotrophic alga, causes growth inhibition in amphibian larvae and has proved refractory to culturein Vitro.P. richardsireplication is dependent on regular passaging through tadpole digestive systems; uptake of thymidine by free-livingProtothecacells and incorporation into DNA are very low by comparison with leucine uptake and incorporation into protein, but DNA synthesis is detectable in cells isolated from tadpole intestines. DNA replication was elicited 6–8 h after ingestion in protothecans fed to tadpoles and subsequently re-isolated from them, providing that the tadpoles were fed subsequent to the ingestion. It appears that passaging through tadpole intestines provides an essential stimulus to maintaining an active cell division cycle inP. richardsi.

scholarly journals Stimulation of DNA synthesis in mouse cell line 3T3 by Simian virus 40.

1966 ◽  
Vol 56 (4) ◽  
pp. 1170-1176 ◽  
Author(s):  
P. Henry ◽  
P. H. Black ◽  
M. N. Oxman ◽  
S. M. Weissman
Keyword(s):  
Dna Synthesis ◽  
Cell Line ◽  
Simian Virus 40 ◽  
Mouse Cell ◽  
Simian Virus ◽  
Mouse Cell Line ◽  
Stimulation Of

DNA Polymerase in Avian Erythroid Cells

1972 ◽  
Vol 11 (3) ◽  
pp. 785-798
Author(s):  
A. F. WILLIAMS
Keyword(s):  
Cell Division ◽  
Dna Synthesis ◽  
Dna Polymerase ◽  
Cell Types ◽  
Polymerase Activity ◽  
Erythroid Cells ◽  
Isolated Nuclei ◽  
Dividing Cells ◽  
Different Cell Types

The level and properties of DNA polymerase activity assayable in extracts of avian erythroid cells was studied. The enzyme was detectable in the dividing cells (erythroblasts) of the erythropoietic series and also the immature non-dividing erythrocytes. It could not be assayed in mature erythrocytes. Investigations showed that activity began to decline at the time of the last cell division of the erythroid series. Properties of the enzyme did change in different cell types; however, the changes did not correlate with cessation of DNA synthesis. Some preliminary results on DNA synthesis by isolated nuclei are also reported and these showed that only nuclei from erythroblasts could synthesize DNA in vitro in the absence of primer.

Hormonal stimulation of myoblast differentiation in the absence of DNA synthesis

1982 ◽  
Vol 243 (5) ◽  
pp. C278-C284 ◽  
Author(s):  
K. A. Turo ◽  
J. R. Florini
Keyword(s):  
Creatine Kinase ◽  
Cell Differentiation ◽  
Dna Synthesis ◽  
Muscle Cell ◽  
Fold Increase ◽  
Cell Types ◽  
Myoblast Differentiation ◽  
Hormonal Stimulation ◽  
Muscle Cell Differentiation ◽  
Stimulation Of

The role of DNA synthesis in the final stages of muscle cell differentiation has been a subject of controversy for more than a decade. In an attempt to resolve disagreements over the necessity for a unique (or "quantal") mitosis just prior to the conversion of proliferating myoblasts to form postmitotic myotubes, we have studied the effects of insulin and somatomedin on the stimulation of myoblast differentiation with or without DNA synthesis. Under conditions in which at least 95% of [3H]thymidine incorporation was blocked by cytosine arabinoside, there was a 5- to 10-fold increase in the extent of differentiation (determined as fusion or creatine kinase elevation) on addition of insulin or multiplication-stimulating activity. The effect of the hormones was on myoblast differentiation, not enzyme induction; insulin did not cause any increase in creatine kinase when it was added to performed myotubes. These studies were done using two different cell types, Yaffe's L6 cell line and Japanese quail myoblasts in serum-free media; we obtained similar results in both. Our results are not compatible with the view that a quantal mitosis is required at a late stage of muscle cell differentiation.

The stimulation of DNA synthesis and cell division in chondrocytes and 3T3 cells by a growth factor isolated from cartilage

1977 ◽  
Vol 105 (1) ◽  
pp. 99-108 ◽  
Author(s):  
M KLAGSBRUN ◽  
R LANGER ◽  
R LEVENSON ◽  
S SMITH ◽  
C LILLEHEI
Keyword(s):  
Cell Division ◽  
Growth Factor ◽  
Dna Synthesis ◽  
3T3 Cells ◽  
Stimulation Of

A propagated stimulation of DNA synthesis and cell division

1961 ◽  
Vol 25 (2) ◽  
pp. 326-340 ◽  
Author(s):  
C.V. Harding ◽  
B.D. Srinivasan
Keyword(s):  
Cell Division ◽  
Dna Synthesis ◽  
Stimulation Of

Benzyladenine-stimulation of nuclear DNA synthesis and cell division in germinating maize

1991 ◽  
Vol 1 (2) ◽  
pp. 113-117 ◽  
Author(s):  
J. Reyes ◽  
L. F. Jiménez-García ◽  
M. A. Gonzalez ◽  
J. M. Vázquez-Ramos
Keyword(s):  
Cell Division ◽  
Dna Replication ◽  
Dna Synthesis ◽  
Mitotic Index ◽  
Nuclear Dna ◽  
Fold Increase ◽  
Mechanisms Of Action ◽  
Dna Metabolism ◽  
Stimulation Of

AbstractWe have studied by means of cytology and autoradiography the effect of benzyladenine (BA, a synthetic cytokinin) on DNA metabolism during early maize germination.The data indicate that BA stimulates nuclear DNA replication. The doubling of the amount of nuclear DNA in BA-treated axes occurs earlier than in nontreated axes, and there is a three-fold increase in the mitotic index at 24 h of germination. These results provide further corroboration for the suggestion that the stimulation of DNA synthesis observed relates to a nuclear replicative type of synthesis. Possible mechanisms of action of BA are discussed.

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