Biochemical, functional, and morphological characterization of a primary culture of rabbit proximal tubule cells

1991 ◽  
Vol 194 (1) ◽  
pp. 9-18 ◽  
Author(s):  
Hervé Toutain ◽  
Nicole Vauclin-Jacques ◽  
Jean-Paul Fillastre ◽  
Jean-Paul Morin
Keyword(s):  
Proximal Tubule ◽  
Primary Culture ◽  
Morphological Characterization ◽  
Proximal Tubule Cells ◽  
Tubule Cells

Cytotoxicity, metabolism and cellular uptake of the mycotoxin deoxynivalenol in human proximal tubule cells and lung fibroblasts in primary culture

Toxicology ◽  
2007 ◽  
Vol 240 (1-2) ◽  
pp. 48-59 ◽  
Author(s):  
Maika Königs ◽  
Marlies Lenczyk ◽  
Gerald Schwerdt ◽  
Hildegard Holzinger ◽  
Michael Gekle ◽  
...  
Keyword(s):  
Proximal Tubule ◽  
Primary Culture ◽  
Cellular Uptake ◽  
Lung Fibroblasts ◽  
Proximal Tubule Cells ◽  
Tubule Cells ◽  
Human Proximal Tubule

Characterization of the beta 2-microglobulin endocytic pathway in rat proximal tubule cells

1994 ◽  
Vol 267 (3) ◽  
pp. F380-F389 ◽  
Author(s):  
D. P. Sundin ◽  
M. Cohen ◽  
R. Dahl ◽  
S. Falk ◽  
B. A. Molitoris
Keyword(s):  
Proximal Tubule ◽  
Endocytic Pathway ◽  
Apical Surface ◽  
Uptake Mechanism ◽  
Proximal Tubule Cells ◽  
Tubule Cells ◽  
Beta 2 ◽  
Beta 2 Microglobulin ◽  
Rat Proximal Tubule

The uptake mechanism(s) of low-molecular-weight proteins by proximal tubule cells remains incompletely characterized. We utilized a biochemical and semiquantitative morphological approach to better characterize the endocytic pathway of an anionic protein, beta 2-microglobulin (beta 2M), in the rat proximal tubule. Indirect immunogold techniques revealed beta 2M was taken up via a classic receptor-mediated endocytic pathway. In vitro biochemical and morphological characterization of iodinated beta 2M and gold-conjugated beta 2M (gold-beta 2M) binding to isolated brush-border membrane vesicles (BBMV) documented specific and quantitatively similar binding interactions of the modified beta 2M with BBMV. Kinetic characterization of the in vivo endocytic pathway of gold-beta 2M was undertaken using microinfusion of individual tubules. beta 2M initially bound at the apical surface, was internalized into subapical coated vesicles and delivered to endosomal-like structures within 5 min, and, finally, was concentrated in lysosomal-like structures within 15 min. This uptake was inhibited by excess unconjugated beta 2M. In addition, we directly showed that uptake did not occur across the basolateral surface. Finally, by passing solubilized BBMV over beta 2M affinity columns we were able to isolate binding activity.

scholarly journals Transcriptomic characterization of short duration endoplasmic reticulum stress on cultured human proximal tubule cells

2014 ◽  
Vol 15 (Suppl 10) ◽  
pp. P5 ◽  
Author(s):  
Yan Zhang ◽  
Michelle Barati ◽  
Ignacio Munoz ◽  
Ming Li ◽  
Danny Wilkey ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Endoplasmic Reticulum Stress ◽  
Proximal Tubule ◽  
Short Duration ◽  
Proximal Tubule Cells ◽  
Tubule Cells ◽  
Human Proximal Tubule

scholarly journals Immortalization and characterization of proximal tubule cells derived from kidneys of spontaneously hypertensive and normotensive rats

1996 ◽  
Vol 50 (1) ◽  
pp. 125-134 ◽  
Author(s):  
Philip G. Woost ◽  
David E. Orosz ◽  
Wenwu Jin ◽  
Phyllis S. Frisa ◽  
James W. Jacobberger ◽  
...  
Keyword(s):  
Proximal Tubule ◽  
Proximal Tubule Cells ◽  
Spontaneously Hypertensive ◽  
Tubule Cells
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