In vitro incubation paralleling changes occurring during mouse cataract formation

1982 ◽  
Vol 35 (5) ◽  
pp. 521-533 ◽  
Author(s):  
Tian-Sheng Hu ◽  
Paul Russell ◽  
Jin H. Kinoshita
Keyword(s):  
Cataract Formation ◽  
In Vitro Incubation

scholarly journals In vitro incubation and study of kidney glomerular epithelial cells

1979 ◽  
Vol 15 (1) ◽  
pp. 80-87 ◽  
Author(s):  
Peter M. Andrews ◽  
Marguerite Stauver
Keyword(s):  
Epithelial Cells ◽  
In Vitro Incubation ◽  
Glomerular Epithelial Cells

scholarly journals Effect of taurine on turkey (Meleagris gallopavo) spermatozoa viability and motility

2018 ◽  
Vol 63 (No. 4) ◽  
pp. 127-135 ◽  
Author(s):  
T. Slanina ◽  
M. Miškeje ◽  
F. Tirpák ◽  
M. Błaszczyk ◽  
R. Stawarz ◽  
...  
Keyword(s):  
Meleagris Gallopavo ◽  
Lower Percentage ◽  
Computer Assisted ◽  
Viability Assessment ◽  
Spermatozoa Motility ◽  
In Vitro Incubation ◽  
Time Periods ◽  
Casa System ◽  
Motility Parameters

The effect of taurine on the turkey spermatozoa motility and viability during the in vitro incubation was assessed. Experimental samples were prepared by diluting the raw semen in nine different concentrations of taurine – from 10 mg/ml to 0.078125 mg/ml. The motility parameters were evaluated by the CASA system (Computer Assisted Semen Analyser) using the program Sperm Vision<sup>®</sup> and for spermatozoa viability assessment the eosin-nigrosin staining was performed. Selected parameters were evaluated at six time periods: 0, 1, 2, 3, 4, and 5 h at 5°C and 41°C. At 5°C, a significantly lower percentage of motility and progressive motility was detected only in the samples with the highest concentration of taurine (10 mg/ml) at time 0 and 1. After 2 h of incubation a significant preventive effect of taurine on spermatozoa parameters was observed. The tendency of the taurine effect on motility parameters was different during the in vitro incubation at 41°C. Significantly lower values of motility parameters were detected in all experimental samples in comparison to the control after 5 h. The analysed concentrations of taurine did not significantly affect viability of turkey spermatozoa during all time periods. A higher percentage of dead spermatozoa were observed at 41°C (4.87–9.90%) if compared to 5°C (2.12–4.88%). The results indicated that the addition of taurine (from 2.5 to 7.5 mg/ml) to turkey spermatozoa positively affected the monitored spermatozoa parameters incubated at 5°C.

An in vitro incubation study of amine-precursor uptake and decarboxylation in the cytoplasm of argyrophil cell adenocarcinoma of the endometrium

1982 ◽  
Vol 14 (1) ◽  
pp. 75-79 ◽  
Author(s):  
Masaki Inoue ◽  
Gaiko Ueda ◽  
Masato Yamasaki ◽  
Yoshiaki Tanaka ◽  
Keizo Hiramatsu ◽  
...  
Keyword(s):  
Argyrophil Cell ◽  
In Vitro Incubation ◽  
Incubation Study
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