Detection of E. coli colonies expressing the v-sis oncogene product with monoclonal antibodies made against synthetic peptides

1985 ◽  
Vol 85 (1) ◽  
pp. 169-182 ◽  
Author(s):  
Roger H. Kennett ◽  
Robert Leunk ◽  
Barbara Meyer ◽  
Vincent Silenzio
Keyword(s):  
Monoclonal Antibodies ◽  
Synthetic Peptides ◽  
E Coli

scholarly journals Monoclonal antibodies specific to a Ca2+-bound form of lipocortin I distinguish its Ca2+-dependent phospholipid-binding ability from its ability to inhibit phospholipase A2

1990 ◽  
Vol 269 (3) ◽  
pp. 709-715 ◽  
Author(s):  
H Hayashi ◽  
M K Owada ◽  
S Sonobe ◽  
K Domae ◽  
T Yamanouchi ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Phospholipase A2 ◽  
Inhibitory Activity ◽  
Biological Effects ◽  
Free Form ◽  
E Coli ◽  
Phospholipid Binding ◽  
Ef Hand

Lipocortin I, a Ca2(+)-and phospholipid-binding protein without EF-hand structures, has many biological effects in vitro. Its actual role in vivo, however is unknown. We obtained and characterized five monoclonal antibodies to lipocortin I. Two of these monoclonal antibodies (L2 and L4-MAbs) reacted with the Ca(+)-bound form of lipocortin I, but not with the Ca2(+)-free form, both in vivo and in vitro. Lipocortin I required greater than or equal to 10 microM-Ca2+ to bind the two antibodies, and this Ca2+ requirement was not affected by phosphatidylserine. L2-MAb abolished the phospholipase A2 inhibitory activity of lipocortin I and inhibited its binding to Escherichia coli membranes and to phosphatidylserine in vitro. L4-MAb abolished the phospholipase A2 inhibitory activity of lipocortin I, but did not affect its binding to E. coli membranes or to phosphatidylserine. These findings indicated that the inhibition of phospholipase A2 by lipocortin I was not simply due to removal or capping of the substrates in E. coli membranes. Furthermore, an immunofluorescence study using L2-MAb showed the actual existence of Ca2(+)-bound form of lipocortin I in vivo.

New monoclonal antibodies as probes for human cardiac troponin I: Epitopic analysis with synthetic peptides

1992 ◽  
Vol 29 (2) ◽  
pp. 271-278 ◽  
Author(s):  
Catherine Larue ◽  
Hélène Defacque-Lacquement ◽  
Charles Calzolari ◽  
Dung Le Nguyen ◽  
Bernard Pau
Keyword(s):  
Monoclonal Antibodies ◽  
Cardiac Troponin ◽  
Synthetic Peptides ◽  
Troponin I ◽  
Cardiac Troponin I

Characterization of Murine Monoclonal Antibodies to HIV-1 Induced by Synthetic Peptides

1988 ◽  
Vol 4 (5) ◽  
pp. 331-342 ◽  
Author(s):  
PAUL J. DURDA ◽  
BARBARA LEECE ◽  
ANNA JENOSKI ◽  
HARVEY RABIN ◽  
AMANDA FISHER ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Synthetic Peptides ◽  
Murine Monoclonal Antibodies ◽  
Hiv 1

Epitope Mapping of Monoclonal Antibodies to Keratin 19 Using Keratin Fragments, Synthetic Peptides and Phage Peptide Libraries

1995 ◽  
Vol 231 (2) ◽  
pp. 475-485 ◽  
Author(s):  
Volker Bottger ◽  
Pauline C. Stasiak ◽  
Dolores L. Harrison ◽  
Dervla M. Mellerick ◽  
E. Birgitte Lane
Keyword(s):  
Monoclonal Antibodies ◽  
Synthetic Peptides ◽  
Epitope Mapping ◽  
Peptide Libraries ◽  
Keratin 19
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