Frameshift mutagenesis by ultraviolet light effects of broth and caffeine in the post-irradiation plating medium

Author(s):  
D.E. McPhee ◽  
Katharine L. Schoeffel
1972 ◽  
Vol 25 (11) ◽  
pp. 2337 ◽  
Author(s):  
JJ Batten

It is fist demonstrated that the occluded solvents and gases which are often present in RDX are expelled prior to zero reaction time of thermal decomposition. Thus they do not seriously interfere with the use of pressure increase as a measure of the extent of thermal decomposition. The rate of thermal decomposition of sublimed RDX at 195�C is then compared with the rate after the following treatments, (i) preliminary grinding of the crystals, (ii) preparation of the crystals by different techniques, (iii) mixing RDX with various solid additives, (iv) interruption of the reaction by cooling, and (v) pre-irradiation with ultraviolet light. The results indicated that treatments (i), (ii), and (iv) had little effect on the rate, whereas (iii) and (v) could markedly influence the rate. Their effects are discussed in terms of the mechanism of decomposition. The results also indicated that there was a post-irradiation reaction of RDX at ambient temperature.


1964 ◽  
Vol 10 (5) ◽  
pp. 717-725 ◽  
Author(s):  
Robert A. Altenbern ◽  
Harold B. Stull

After ultraviolet light irradiation, strain 6464 of Bacillus cereus lysed, resulting in the release of toxin, phospholipase, and mature phage particles. Small amounts of toxin and phospholipase produced by non-induced cultures were correlated with the infrequent spontaneous release of bacteriophage. Stationary incubation following ultraviolet induction results in a greater yield of toxin and phospholipase than post irradiation incubation on a shaker. Postirradiation incubation at temperatures below 37° either reduced (30°) or prevented (26°) toxin and phospholipase production. A clone was obtained which was sensitive to the phage from the parent strain and was presumably no longer lysogenic for it. This cured strain still exhibited ultraviolet-induced optical density decline accompanied by release of toxin and phospholipase. Mitomycin C would induce strain 6464 and the cured strain derived from it and both released toxin and phospholipase during mitomycin C induced lysis. The induced lysis of the cured strain could be prevented by postinduction treatment with inhibitors of synthesis of protein (chloramphenicol), RNA (actinomycin D), or DNA (5-fluorouracil deoxyriboside).


Transfusion ◽  
2009 ◽  
Vol 49 (10) ◽  
pp. 2167-2172 ◽  
Author(s):  
Valerie S. Hornsey ◽  
Olive Drummond ◽  
Alex Morrison ◽  
Loraine McMillan ◽  
Ian R. MacGregor ◽  
...  

1963 ◽  
Vol 61 (1) ◽  
pp. 77-82 ◽  
Author(s):  
Mitsuru Nakamura ◽  
Carol M. Ramage

More cells ofShigella sonneiwere recovered on a chemically defined medium than on complex media after the cells were irradiated with ultraviolet light. The temperature of incubation affected the numbers of colony-forming cells that could be recovered. A temperature of 37° C. yielded the largest number of colonies compared with temperature of 15, 27 and 44° C. Recovery of irradiated cells was favoured when the cells were held in buffer at 25° C. for 2½ hr. before plating on solid media.


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