The relationship between DNA damage and mutation frequency in mammalian cell lines treated with N-nitroso-N-2-fluorenylacetamide

1989 ◽  
Vol 212 (2) ◽  
pp. 231-239 ◽  
Author(s):  
Ming-Liang Kuo ◽  
Jen-Kun Lin
Keyword(s):  
Dna Damage ◽  
Cell Lines ◽  
Mammalian Cell ◽  
Mutation Frequency ◽  
Mammalian Cell Lines ◽  
The Relationship

Comparative analysis of micronuclei and DNA damage induced by Ochratoxin A in two mammalian cell lines

2011 ◽  
Vol 723 (1) ◽  
pp. 58-64 ◽  
Author(s):  
Rahat Ali ◽  
Roberta A. Mittelstaedt ◽  
Joseph G. Shaddock ◽  
Wei Ding ◽  
Javed A. Bhalli ◽  
...  
Keyword(s):  
Dna Damage ◽  
Comparative Analysis ◽  
Ochratoxin A ◽  
Cell Lines ◽  
Mammalian Cell ◽  
Mammalian Cell Lines

DNA damage in mammalian cell lines with different antioxidant levels and DNA repair capacities

1992 ◽  
pp. 247-250
Author(s):  
Bernadette M. Hannigan ◽  
Shirley-Ann M. Richardson ◽  
P. Gerald McKenna
Keyword(s):  
Dna Damage ◽  
Dna Repair ◽  
Cell Lines ◽  
Mammalian Cell ◽  
Mammalian Cell Lines

Ochratoxin A: induction of (oxidative) DNA damage, cytotoxicity and apoptosis in mammalian cell lines and primary cells

Toxicology ◽  
2005 ◽  
Vol 206 (3) ◽  
pp. 413-425 ◽  
Author(s):  
H KAMP ◽  
G EISENBRAND ◽  
J SCHLATTER ◽  
K WURTH ◽  
C JANZOWSKI
Keyword(s):  
Dna Damage ◽  
Ochratoxin A ◽  
Cell Lines ◽  
Mammalian Cell ◽  
Oxidative Dna Damage ◽  
Primary Cells ◽  
Mammalian Cell Lines

scholarly journals Structure-guided selection of puromycin N-acetyltransferase mutants with enhanced selection stringency for deriving mammalian cell lines expressing recombinant proteins

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Alessandro T. Caputo ◽  
Oliver M. Eder ◽  
Hana Bereznakova ◽  
Heleen Pothuis ◽  
Albert Ardevol ◽  
...  
Keyword(s):  
Cell Lines ◽  
Mammalian Cell ◽  
Recombinant Proteins ◽  
Cultured Cells ◽  
Hek293 Cells ◽  
Reaction Products ◽  
Enzyme Form ◽  
X Ray Crystallography ◽  
Mammalian Cell Lines ◽  
Apparent Loss

AbstractPuromycin and the Streptomyces alboniger-derived puromycin N-acetyltransferase (PAC) enzyme form a commonly used system for selecting stably transfected cultured cells. The crystal structure of PAC has been solved using X-ray crystallography, revealing it to be a member of the GCN5-related N-acetyltransferase (GNAT) family of acetyltransferases. Based on structures in complex with acetyl-CoA or the reaction products CoA and acetylated puromycin, four classes of mutations in and around the catalytic site were designed and tested for activity. Single-residue mutations were identified that displayed a range of enzymatic activities, from complete ablation to enhanced activity relative to wild-type (WT) PAC. Cell pools of stably transfected HEK293 cells derived using two PAC mutants with attenuated activity, Y30F and A142D, were found to secrete up to three-fold higher levels of a soluble, recombinant target protein than corresponding pools derived with the WT enzyme. A third mutant, Y171F, appeared to stabilise the intracellular turnover of PAC, resulting in an apparent loss of selection stringency. Our results indicate that the structure-guided manipulation of PAC function can be utilised to enhance selection stringency for the derivation of mammalian cell lines secreting elevated levels of recombinant proteins.

Sign in / Sign up

Export Citation Format

Share Document