Serodiagnosis of human cysticercosis using a chromatofocused antigenic preparation of Taenia solium cysticerci in an enzyme-linked immunosorbent assay (ELISA)

1984 ◽  
Vol 78 (4) ◽  
pp. 492-496 ◽  
Author(s):  
Millicent Coker-Vann ◽  
Paul Brown ◽  
D.Carleton Gajdusek
Keyword(s):  
Immunosorbent Assay ◽  
Taenia Solium ◽  
Enzyme Linked Immunosorbent Assay ◽  
Antigenic Preparation

scholarly journals Development of an Enzyme-Linked Immunosorbent Assay for Serotyping Ureaplasma urealyticum Strains Using Monoclonal Antibodies

2001 ◽  
Vol 8 (1) ◽  
pp. 52-57 ◽  
Author(s):  
Fedoua Echahidi ◽  
Gaëtan Muyldermans ◽  
Sabine Lauwers ◽  
Anne Naessens
Keyword(s):  
Monoclonal Antibodies ◽  
Immunosorbent Assay ◽  
Ureaplasma Urealyticum ◽  
Polyclonal Antibodies ◽  
Enzyme Linked Immunosorbent Assay ◽  
Elisa Method ◽  
Cross Reactions ◽  
Antigenic Preparation ◽  
Negative Controls ◽  
Reference Strains

ABSTRACT Ureaplasma urealyticum comprises 14 serotypes. The existing serotyping methods all use polyclonal antibodies. These methods are time-consuming and labor-intensive, and they cannot always be performed on primary isolates; in addition, the results are difficult to interpret. We developed a new enzyme-linked immunosorbent assay (ELISA) method using serotype-specific monoclonal antibodies (MAbs) to enable the serotyping of U. urealyticum isolates from primary broth cultures. Each of the 14 serotype reference strains was tested against 14 selected MAbs. Homologous reactions were very strong, while heterologous reactions were negligible. Three cross-reactions were observed: MAb 5 cross-reacted with serotype 2, MAb 14 cross-reacted with serotype 3, and MAb 8 cross-reacted with serotype 13. Despite the cross-reactions observed, all the serotype reference strains ofU. urealyticum could be identified and differentiated using a combination of MAbs. Reproducibility was analyzed with a fractionated antigenic preparation and with several freshly prepared antigens of the same strain. No significant interrun variation was found with the fractionated antigen, but significant variations in optical density (OD) values were found when freshly prepared antigens were tested. However, the variation in OD values did not influence the overall interpretation of the ELISA: reactions with homologous MAbs were always prominent compared to those of the negative controls. This newly developed ELISA using MAbs seems promising for serotyping of U. urealyticum clinical isolates.

scholarly journals Evidence for Transmission of Taenia solium Taeniasis/Cysticercosis in a Rural Area of Northern Rwanda

2021 ◽  
Vol 8 ◽  
Author(s):  
Lucrecia Acosta Soto ◽  
Lucy Anne Parker ◽  
María José Irisarri-Gutiérrez ◽  
Javier Arturo Bustos ◽  
Yesenia Castillo ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Low Income ◽  
Taenia Solium ◽  
Parasitic Infection ◽  
Developed Countries ◽  
Cross Sectional Study ◽  
Low Income Countries ◽  
Enzyme Linked Immunosorbent Assay ◽  
Cross Sectional ◽  
Hygiene Measures

Cysticercosis is a parasitic infection caused by the metacestode larval stage (cysticercus) of Taenia solium. In humans, cysticercosis may infect the central nervous system and cause neurocysticercosis, which is responsible for over 50,000 deaths per year worldwide and is the major cause of preventable epilepsy cases, especially in low-income countries. Cysticercosis infection is endemic in many less developed countries where poor hygiene conditions and free-range pig management favor their transmission. A cross-sectional study was conducted in 680 children from a rural primary school in Gakenke district (Northern province of Rwanda). Stool samples were collected from participants and analyzed using the Kato-Katz method (KK), formol-ether concentration (FEC), and/or copro-antigen enzyme-linked immunosorbent assay (CoAg-ELISA) to detect taeniasis. Blood samples were collected and analyzed using enzyme-linked immunoelectrotransfer blot (EITB) and antigen enzyme-linked immunosorbent assay (Ag-ELISA) to detect human cysticercosis. The overall proportion of taeniasis positivity was 0.3% (2/680), and both cases were also confirmed by CoAg-ELISA. A total of 13.3% (76/572) of the children studied were positive to cysticercosis (T. solium-specific serum antibodies detected by EITB), of whom 38.0% (27/71) had viable cysticercus (T. solium antigens by Ag-ELISA). This study provides evidence of the highest cysticercosis prevalence reported in Rwanda in children to date. Systematic investigations into porcine and human cysticercosis as well as health education and hygiene measures for T. solium control are needed in Gakenke district.

scholarly journals Frequency of serum anti-cysticercus antibodies in the population of a rural Brazilian community (Cássia dos Coqueiros, SP) determined by ELISA and immunoblotting using Taenia crassiceps antigens

2002 ◽  
Vol 44 (1) ◽  
pp. 7-12 ◽  
Author(s):  
Lúcia M. BRAGAZZA ◽  
Adelaide J. VAZ ◽  
Afonso D.C. PASSOS ◽  
Osvaldo M. TAKAYANAGUI ◽  
Paulo M. NAKAMURA ◽  
...  
Keyword(s):  
Public Health ◽  
Immunosorbent Assay ◽  
Taenia Solium ◽  
High Specificity ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Taenia Crassiceps ◽  
Municipal District ◽  
The Impact

Considering the impact of cysticercosis on public health, especially the neurologic form of the disease, neurocysticercosis (NC), we studied the frequency of positivity of anti-Taenia solium cysticercus antibodies in serum samples from 1,863 inhabitants of Cássia dos Coqueiros, SP, a municipal district located 80 km from Ribeirão Preto, an area considered endemic for cysticercosis. The 1,863 samples were tested by enzyme linked immunosorbent assay (ELISA) using an antigenic extract from Taenia crassiceps vesicular fluid (Tcra). The reactive and inconclusive ELISA samples were tested by immunoblotting. Of the 459 samples submitted to immunoblotting, 40 were strongly immunoreactive to the immunodominant 18 and 14 kD peptides. Considering the use of immunoblotting as confirmatory due to its high specificity, the anti-cysticercus serum prevalence in this population was 2.1%.

Enzyme-Linked Immunosorbent Assay (Elisa) for the Detection of Antibody to Cysticerci of Taenia Solium

1982 ◽  
Vol 31 (2) ◽  
pp. 364-369 ◽  
Author(s):  
Arwin R. Diwan ◽  
Millicent Coker-Vann ◽  
Paul Brown ◽  
D. B. Subianto ◽  
D. Carleton Gajdusek ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Taenia Solium ◽  
Enzyme Linked Immunosorbent Assay

Discrimination between active and inactive neurocysticercosis by metacestode excretory/secretory antigens of Taenia solium in an enzyme-linked immunosorbent assay.

2002 ◽  
Vol 66 (6) ◽  
pp. 777-781 ◽  
Author(s):  
José L Molinari ◽  
Julio Sotelo ◽  
Patricia Tato ◽  
Yazmin de la Garza ◽  
Esperanza García-Mendoza ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Taenia Solium ◽  
Enzyme Linked Immunosorbent Assay

scholarly journals Seroprevalensi Sistiserkosis pada Babi di Wilayah Wamena, Papua

2020 ◽  
pp. 67
Author(s):  
Alvionita Alexther Lingga ◽  
Ida Bagus Ngurah Swacita ◽  
I Ketut Suada
Keyword(s):  
Immunosorbent Assay ◽  
Taenia Solium ◽  
Enzyme Linked Immunosorbent Assay

Sistiserksosis merupakan penyakit zoonosis disebabkan oleh larva cacing Taenia solium yang berdampak serius pada kesehatan hewan maupun manusia. Papua merupakan salah satu daerah endemis sistiserkosis di Indonesia. Penelitian ini bertujuan untuk mengetahui seroprevalensi sistiserkosis pada babi di wilayah Wamena, Papua. Sebanyak seratus sebelas sampel serum babi yang disampling dari wilayah Wamena kemudian diuji dengan Enzyme Linked Immunosorbent Assay (ELISA). Hasil penelitian menunjukkan bahwa seroprevalensi sistiserkosis pada sampel babi tersebut sebanyak 39,64% (44/111). Dapat disimpulkan bahwa seroprevalensi sistiserkosis pada sampel babi dari Wilayah Wamena, Papua sangat tinggi, dengan demikian maka diperlukan program untuk memberantas penyakit ini oleh instansi terkait di Wilayah Wamena, Papua

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