Discrimination between active and inactive neurocysticercosis by metacestode excretory/secretory antigens of Taenia solium in an enzyme-linked immunosorbent assay.

2002 ◽  
Vol 66 (6) ◽  
pp. 777-781 ◽  
Author(s):  
José L Molinari ◽  
Julio Sotelo ◽  
Patricia Tato ◽  
Yazmin de la Garza ◽  
Esperanza García-Mendoza ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Taenia Solium ◽  
Enzyme Linked Immunosorbent Assay

scholarly journals Evidence for Transmission of Taenia solium Taeniasis/Cysticercosis in a Rural Area of Northern Rwanda

2021 ◽  
Vol 8 ◽  
Author(s):  
Lucrecia Acosta Soto ◽  
Lucy Anne Parker ◽  
María José Irisarri-Gutiérrez ◽  
Javier Arturo Bustos ◽  
Yesenia Castillo ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Low Income ◽  
Taenia Solium ◽  
Parasitic Infection ◽  
Developed Countries ◽  
Cross Sectional Study ◽  
Low Income Countries ◽  
Enzyme Linked Immunosorbent Assay ◽  
Cross Sectional ◽  
Hygiene Measures

Cysticercosis is a parasitic infection caused by the metacestode larval stage (cysticercus) of Taenia solium. In humans, cysticercosis may infect the central nervous system and cause neurocysticercosis, which is responsible for over 50,000 deaths per year worldwide and is the major cause of preventable epilepsy cases, especially in low-income countries. Cysticercosis infection is endemic in many less developed countries where poor hygiene conditions and free-range pig management favor their transmission. A cross-sectional study was conducted in 680 children from a rural primary school in Gakenke district (Northern province of Rwanda). Stool samples were collected from participants and analyzed using the Kato-Katz method (KK), formol-ether concentration (FEC), and/or copro-antigen enzyme-linked immunosorbent assay (CoAg-ELISA) to detect taeniasis. Blood samples were collected and analyzed using enzyme-linked immunoelectrotransfer blot (EITB) and antigen enzyme-linked immunosorbent assay (Ag-ELISA) to detect human cysticercosis. The overall proportion of taeniasis positivity was 0.3% (2/680), and both cases were also confirmed by CoAg-ELISA. A total of 13.3% (76/572) of the children studied were positive to cysticercosis (T. solium-specific serum antibodies detected by EITB), of whom 38.0% (27/71) had viable cysticercus (T. solium antigens by Ag-ELISA). This study provides evidence of the highest cysticercosis prevalence reported in Rwanda in children to date. Systematic investigations into porcine and human cysticercosis as well as health education and hygiene measures for T. solium control are needed in Gakenke district.

scholarly journals Frequency of serum anti-cysticercus antibodies in the population of a rural Brazilian community (Cássia dos Coqueiros, SP) determined by ELISA and immunoblotting using Taenia crassiceps antigens

2002 ◽  
Vol 44 (1) ◽  
pp. 7-12 ◽  
Author(s):  
Lúcia M. BRAGAZZA ◽  
Adelaide J. VAZ ◽  
Afonso D.C. PASSOS ◽  
Osvaldo M. TAKAYANAGUI ◽  
Paulo M. NAKAMURA ◽  
...  
Keyword(s):  
Public Health ◽  
Immunosorbent Assay ◽  
Taenia Solium ◽  
High Specificity ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Taenia Crassiceps ◽  
Municipal District ◽  
The Impact

Considering the impact of cysticercosis on public health, especially the neurologic form of the disease, neurocysticercosis (NC), we studied the frequency of positivity of anti-Taenia solium cysticercus antibodies in serum samples from 1,863 inhabitants of Cássia dos Coqueiros, SP, a municipal district located 80 km from Ribeirão Preto, an area considered endemic for cysticercosis. The 1,863 samples were tested by enzyme linked immunosorbent assay (ELISA) using an antigenic extract from Taenia crassiceps vesicular fluid (Tcra). The reactive and inconclusive ELISA samples were tested by immunoblotting. Of the 459 samples submitted to immunoblotting, 40 were strongly immunoreactive to the immunodominant 18 and 14 kD peptides. Considering the use of immunoblotting as confirmatory due to its high specificity, the anti-cysticercus serum prevalence in this population was 2.1%.

Enzyme-Linked Immunosorbent Assay (Elisa) for the Detection of Antibody to Cysticerci of Taenia Solium

1982 ◽  
Vol 31 (2) ◽  
pp. 364-369 ◽  
Author(s):  
Arwin R. Diwan ◽  
Millicent Coker-Vann ◽  
Paul Brown ◽  
D. B. Subianto ◽  
D. Carleton Gajdusek ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Taenia Solium ◽  
Enzyme Linked Immunosorbent Assay

scholarly journals Seroprevalensi Sistiserkosis pada Babi di Wilayah Wamena, Papua

2020 ◽  
pp. 67
Author(s):  
Alvionita Alexther Lingga ◽  
Ida Bagus Ngurah Swacita ◽  
I Ketut Suada
Keyword(s):  
Immunosorbent Assay ◽  
Taenia Solium ◽  
Enzyme Linked Immunosorbent Assay

Sistiserksosis merupakan penyakit zoonosis disebabkan oleh larva cacing Taenia solium yang berdampak serius pada kesehatan hewan maupun manusia. Papua merupakan salah satu daerah endemis sistiserkosis di Indonesia. Penelitian ini bertujuan untuk mengetahui seroprevalensi sistiserkosis pada babi di wilayah Wamena, Papua. Sebanyak seratus sebelas sampel serum babi yang disampling dari wilayah Wamena kemudian diuji dengan Enzyme Linked Immunosorbent Assay (ELISA). Hasil penelitian menunjukkan bahwa seroprevalensi sistiserkosis pada sampel babi tersebut sebanyak 39,64% (44/111). Dapat disimpulkan bahwa seroprevalensi sistiserkosis pada sampel babi dari Wilayah Wamena, Papua sangat tinggi, dengan demikian maka diperlukan program untuk memberantas penyakit ini oleh instansi terkait di Wilayah Wamena, Papua

scholarly journals Serological Diagnosis of Human Cysticercosis by Use of Recombinant Antigens from Taenia solium Cysticerci

1999 ◽  
Vol 6 (4) ◽  
pp. 479-482 ◽  
Author(s):  
Kerstin Hubert ◽  
Abel Andriantsimahavandy ◽  
Alain Michault ◽  
Matthias Frosch ◽  
Fritz A. Mühlschlegel
Keyword(s):  
Immunosorbent Assay ◽  
Fusion Proteins ◽  
Taenia Solium ◽  
Serological Diagnosis ◽  
Enzyme Linked Immunosorbent Assay ◽  
Cdna Expression Library ◽  
Serum Samples ◽  
Expression Library ◽  
Recombinant Antigens ◽  
Cdna Expression

ABSTRACT A Taenia solium metacestode cDNA expression library in the lambda ZAPII vector was screened with pooled sera from patients with neurocysticercosis. Sixty primary clones were identified and shown to belong to two classes. The clones NC-3 and NC-9 did not reveal any significant homologies to sequences deposited in the databases and were further characterized. Both recombinant antigens were expressed as glutathione S-transferase fusion proteins and applied for serological diagnosis of human cysticercosis. An enzyme-linked immunosorbent assay was established and evaluated with 27 serum samples of La Réunion and Madagascar patients with cysticercosis. Diagnosis in these patients was established with radiological and serological procedures. For antigen NC-3 a sensitivity of 96.3% and a specificity of 91.5% for the serodiagnosis were achieved. In contrast, the sensitivity of antigen NC-9 was only 33.3%.

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