Capillary electrophoresis: A highly sensitive technique for the determination of marine phycotoxins

A highly sensitive and stable solid-state electrochemiluminescence (ECL) sensor was developed based on tris(2,2’-bipyridyl)ruthenium(II) (Ru(bpy)32+) integrating with 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) functionalized graphene. Ru(bpy)32+ is incorporated with the ABTS functionalized graphene based on not only the π-π stacking but also electrostatic interactions. Coupled with capillary electrophoresis (CE), this ECL sensor was used to detect tyramine and 5-hydroxytryptamine (5-HT) based on their quenching effects for the Ru(bpy)32+/tripropylamine (TPA) system. The quenching mechanism was illustrated and the conditions for CE separation and ECL detection were optimized. Based on an S/N = 3, the limit of detection (LOD) for tyramine and 5-HT were 0.1 μM and 0.02 μM, respectively. The applicability of the proposed method was further illustrated in the determination of tyramine and 5-HT in human plasma samples from small intestine carcinoid patients.

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Highly Sensitive Determination of Total Dissolved Aluminum in Water Samples Using Capillary Electrophoresis with On-Line Preconcentration Method

BUNSEKI KAGAKU ◽

10.2116/bunsekikagaku.56.479 ◽

2007 ◽

Vol 56 (6) ◽

pp. 479-483 ◽

Cited By ~ 1

Author(s):

Young-Sik HAM ◽

Sayaka TAMIYA ◽

I-Song CHOI

Keyword(s):

Capillary Electrophoresis ◽

Water Samples ◽

Highly Sensitive ◽

Dissolved Aluminum ◽

On Line ◽

Sensitive Determination ◽

Preconcentration Method

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Highly sensitive determination of aminoglycoside residues in food by sheathless CE-ESI-MS/MS

Analytical Methods ◽

10.1039/c9ay01728c ◽

2019 ◽

Vol 11 (39) ◽

pp. 5064-5069 ◽

Cited By ~ 3

Author(s):

Yue Yu ◽

Ying Liu ◽

Wentao Wang ◽

Yanbo Jia ◽

Guiping Zhao ◽

...

Keyword(s):

Mass Spectrometry ◽

Capillary Electrophoresis ◽

Tandem Mass Spectrometry ◽

Electrospray Ionization ◽

Tandem Mass ◽

Spectrometry Method ◽

Highly Sensitive ◽

Sensitive Determination ◽

Ionization Tandem Mass Spectrometry

A highly sensitive sheathless capillary electrophoresis-electrospray ionization-tandem mass spectrometry method and transient isotachophoresis was established for the determination of aminoglycoside antibiotics in milk.

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Determination of Molecular Weight of Silk Fibroin by Non-Gel Sieving Capillary Electrophoresis

Journal of AOAC International ◽

10.1093/jaoac/93.4.1143 ◽

2010 ◽

Vol 93 (4) ◽

pp. 1143-1147 ◽

Cited By ~ 1

Author(s):

Wei Wei ◽

Yaopeng Zhang ◽

Huili Shao ◽

Xuechao Hu

Keyword(s):

Capillary Electrophoresis ◽

Silk Fibroin ◽

Gel Electrophoresis ◽

Polyacrylamide Gel Electrophoresis ◽

Background Electrolyte ◽

Sodium Dodecyl ◽

Absolute Value ◽

Regenerated Silk Fibroin ◽

Highly Sensitive

Abstract A simple non-gel sieving capillary electrophoresis (NGSCE) method was established to determine the MW of silk fibroin using CE. The background electrolyte with a pH of 8.8 was based on three components: polyethylene glycol, tris(hydroxymethyl)aminomethane, and sodium dodecyl sulfate (SDS). NGSCE showed a good linear relationship with satisfactory reproducibility between the migration time and the MW of standard proteins. It was found that the regenerated silk fibroin had an MW around 83 kDa with a wide MW distribution (MWD). This absolute value is lower than the result obtained from SDS-polyacrylamide gel electrophoresis due to the different principles of the methods, but their similar MWD shapes indicated that NGSCE could be a feasible, highly sensitive, rapid method for determination of the MW of silk fibroin.

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