Lesions of the nucleus basalis magnocellularis in immature rats: Short- and long-term biochemical and behavioral changes

Communication with bacteria deeply impacts the life history traits of their hosts. Through specific molecules and metabolites, bacteria can promote short- and long-term phenotypic and behavioral changes in the nematode Caenorhabditis elegans. The chronic exposure of C. elegans to pathogens promotes the adaptive behavior in the host’s progeny called pathogen-induced diapause formation (PIDF). PIDF is a pathogen avoidance strategy induced in the second generation of animals infected and can be recalled transgenerationally. This behavior requires the RNA interference machinery and specific nematode and bacteria small RNAs (sRNAs). In this work, we assume that RNAs from both species co-exist and can interact with each other. Under this principle, we explore the potential interspecies RNA interactions during PIDF-triggering conditions, using transcriptomic data from the holobiont. We study two transcriptomics datasets: first, the dual sRNA expression of Pseudomonas aeruginosa PAO1 and C. elegans in a transgenerational paradigm for six generations and second, the simultaneous expression of sRNAs and mRNA in intergenerational PIDF. We focus on those bacterial sRNAs that are systematically overexpressed in the intestines of animals compared with sRNAs expressed in host-naïve bacteria. We selected diverse in silico methods that represent putative mechanisms of RNA-mediated interspecies interaction. These interactions are as follows: heterologous perfect and incomplete pairing between bacterial RNA and host mRNA; sRNAs of similar sequence expressed in both species that could mimic each other; and known or predicted eukaryotic motifs present in bacterial transcripts. We conclude that a broad spectrum of tools can be applied for the identification of potential sRNA and mRNA targets of the interspecies RNA interaction that can be subsequently tested experimentally.

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Electrolytic and ibotenic acid lesions of the nucleus basalis magnocellularis interrupt long-term retention, but not acquisition of two-way active avoidance, in rats

Experimental Brain Research ◽

10.1007/s00221-001-0917-4 ◽

2002 ◽

Vol 142 (1) ◽

pp. 52-66 ◽

Cited By ~ 21

Author(s):

Anna Vale-Martínez ◽

Gemma Guillazo-Blanch ◽

Margarita Martí-Nicolovius ◽

Roser Nadal ◽

Rosa Arévalo-García ◽

...

Keyword(s):

Active Avoidance ◽

Ibotenic Acid ◽

Nucleus Basalis ◽

Nucleus Basalis Magnocellularis ◽

Term Retention ◽

Long Term Retention

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Effect of intra-hippocampal injection of human recombinant growth hormone on synaptic plasticity in the nucleus basalis magnocellularis-lesioned aged rats

Arquivos de Neuro-Psiquiatria ◽

10.1590/0004-282x20170074 ◽

2017 ◽

Vol 75 (7) ◽

pp. 477-483 ◽

Cited By ~ 2

Author(s):

Maryam Malek ◽

Alireza Sarkaki ◽

Saleh Zahedi-Asl ◽

Yaghoob Farbood ◽

Ziba Rajaei

Keyword(s):

Growth Hormone ◽

Synaptic Plasticity ◽

Long Term Potentiation ◽

Nucleus Basalis ◽

Vehicle Group ◽

High Frequency Stimulation ◽

Nucleus Basalis Magnocellularis ◽

Term Potentiation ◽

Intrahippocampal Injection

ABSTRACT In this study, we proposed that administration of hippocampal growth hormone in ageing animals with growth hormone deficiency can compensate long-term potentiation and synaptic plasticity in nucleus basalis magnocellularis (NBM)-lesioned rats. Aged male Wistar rats were randomly divided into six groups (seven in each) of sham-operated healthy rats (Cont); NBM-lesioned rats (L); NBM-lesioned rats and intrahippocampal injection of growth hormone vehicle (L + Veh); NBM-lesioned and intrahippocampal injection of growth hormone (10, 20 and 40 µg.2 µl-1) (L + GH). In vivo electrophysiological recording techniques were used to characterize maintenance of long-term potentiation at distinct times (1, 2, 3, 24 and 48 hours) after high-frequency stimulation. The population spike was enhanced significantly for about 48 hours following tetanic stimulation in rats treated with a dose-dependent growth hormone compared to the vehicle group (p < 0.05), possibly through neuronal plasticity and neurogenesis in affected areas.

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Short- and long-term effects of an LHRH antagonist given during the prepubertal period on follicle dynamics in the rat

Journal of Endocrinology ◽

10.1677/joe.0.1240247 ◽

1990 ◽

Vol 124 (2) ◽

pp. 247-253 ◽

Cited By ~ 8

Author(s):

H. M. A. Meijs-Roelofs ◽

W. A. van Cappellen ◽

E. C. M. van Leeuwen ◽

P. Kramer

Keyword(s):

Ovarian Follicle ◽

Corpora Lutea ◽

Long Term Effects ◽

Primordial Follicles ◽

Immature Rats ◽

Lhrh Antagonist ◽

Early Peak ◽

Short And Long Term ◽

And Control

ABSTRACT The effects of the suppression of the high gonadotrophin concentrations normally present by the end of the second week of life on ovarian follicle dynamics were studied in immature rats. Gonadotrophins were suppressed by treatment with an LHRH antagonist (LHRH-A; Org. 30276) on days 6, 9, 12 and 15, and the total population of ovarian follicles was studied at 15 and 28 days, on the day of first oestrus and on the day of oestrus at or following 90 and 300 days of age. Primordial follicles were counted and growing follicles were counted and measured. In rats treated with LHRH-A, follicle recruitment into the growing pool was clearly diminished; the number of growing follicles was significantly (P<0·01) lower up to the day of first oestrus and the pool of primordial follicles was significantly (P<0·05) larger at 15 and 28 days. Ovarian weights were significantly lower in rats treated with LHRH-A until at least 90 days of age. However, on the day of oestrus at or after 90 and 300 days of age, there were no differences in either the pool of primordial follicles or the pool of growing follicles between rats treated with LHRH-A and control rats. There was also no difference between groups in the number of fresh corpora lutea at these ages. It was concluded that the early peak in gonadotrophin concentrations in immature rats causes substantial recruitment of follicles into the growing pool. Thus, the number of follicles entering the growing pool is not solely dependent upon the size of the pool of primordial follicles but is clearly influenced by the level of circulating gonadotrophins. In contrast, the large gonadotrophic stimulation that normally takes place during the second and third week of life is neither a prerequisite for functional sexual maturation nor for later cyclic function. Shortly before the time of first ovulation a tight control of follicle dynamics is established which is largely independent of previous gonadotrophin concentrations and follicle dynamics. Journal of Endocrinology (1990) 124, 247–253

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