Ribosomal precursor 3′ end formation requires a conserved element upstream of the promoter

Cell ◽  
1987 ◽  
Vol 50 (1) ◽  
pp. 51-57 ◽  
Author(s):  
Paul Labhart ◽  
Ronald H. Reeder
Keyword(s):  
Ribosomal Precursor

Control points in eucaryotic ribosome biogenesis

1991 ◽  
Vol 69 (1) ◽  
pp. 5-22 ◽  
Author(s):  
D. E. Larson ◽  
P. Zahradka ◽  
B. H. Sells
Keyword(s):  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Ribosomal Proteins ◽  
Ribosome Biogenesis ◽  
Rna Polymerase Iii ◽  
Ribosomal Subunits ◽  
Rrna Species ◽  
Polymerase I ◽  
Ribosomal Precursor ◽  
Precursor Molecules

Ribosome biogenesis in eucaryotic cells involves the coordinated synthesis of four rRNA species, transcribed by RNA polymerase I (18S, 28S, 5.8S) and RNA polymerase III (5S), and approximately 80 ribosomal proteins translated from mRNAs synthesized by RNA polymerase II. Assembly of the ribosomal subunits in the nucleolus, the site of 45S rRNA precursor gene transcription, requires the movement of 5S rRNA and ribosomal proteins from the nucleoplasm and cytoplasm, respectively, to this structure. To integrate these events and ensure the balanced production of individual ribosomal components, different strategies have been developed by eucaryotic organisms in response to a variety of physiological changes. This review presents an overview of the mechanisms modulating the production of ribosomal precursor molecules and the rate of ribosome biogenesis in various biological systems.Key words: rRNA, ribosomal proteins, nucleolus, ribosome.

Ribosomal precursor particles in ascites tumor cell nucleoli

1973 ◽  
Vol 81 (1) ◽  
pp. 1-7 ◽  
Author(s):  
R. Simard ◽  
Françoise Sakr ◽  
J.-P. Bachellerie
Keyword(s):  
Tumor Cell ◽  
Ascites Tumor ◽  
Ribosomal Precursor

Effect of Cordycepin on Ribosome Formation and Enzyme Induction in Rat Liver

1972 ◽  
Vol 50 (9) ◽  
pp. 1010-1015 ◽  
Author(s):  
Arthur J. Rizzo ◽  
Camille Kelly ◽  
Thomas E. Webb
Keyword(s):  
Rat Liver ◽  
Messenger Rna ◽  
Nucleocytoplasmic Transport ◽  
Current Theory ◽  
Polyadenylic Acid ◽  
General Protein Synthesis ◽  
Tyrosine Transaminase ◽  
General Protein ◽  
Nuclear Processing ◽  
Ribosomal Precursor

Cordycepin (3′-deoxyadenosine), at a dose of 10 mg/kg, inhibits the accumulation of ribosomes in rat liver cytoplasm within 2.5 h of administration. This effect is not due to an inhibition of RNA synthesis per se but rather to the premature termination of the transcription of the 45 S ribosomal precursor in the presence of the analogue. The nucleoside analogue also inhibits the corticosteroid-mediated induction of tyrosine transaminase and general protein synthesis. The latter results are consistent with the current theory that cordycepin inhibits the formation of polyadenylic acid which is involved in the nuclear processing and/or nucleocytoplasmic transport of messenger RNA.

scholarly journals Ribosomal ribonucleic acid and ribosomal precursor ribonucleic acid in Anacystis nidulans

1972 ◽  
Vol 129 (1) ◽  
pp. 135-140 ◽  
Author(s):  
A. Szalay ◽  
D. Munsche ◽  
R. Wollgiehn ◽  
B. Parthier
Keyword(s):  
Ribonucleic Acid ◽  
Extraction Procedure ◽  
Anacystis Nidulans ◽  
Pulse Labelling ◽  
Blue Green Algae ◽  
Molecular Weights ◽  
Close Relationship ◽  
Rrna Species ◽  
Precursor Rna ◽  
Ribosomal Precursor

The RNA of the blue–green alga Anacystis nidulans contains three ribosomal RNA species with molecular weights of 0.56×106, 0.9×106, and 1.1×106if the RNA is extracted in the absence of Mg2+. The 0.9×106mol.wt. rRNA is extremely slowly labelled in32P-incorporation experiments. This rRNA may be a cleavage product of the 1.1×106mol.wt. rRNA from the ribosomes of cells in certain physiological states (e.g. light-deficiency during growth). The cleavage of the 1.1×106mol.wt. rRNA during the extraction procedure can be prevented by the addition of 10mm-MgCl2.32P-pulse-labelling studies demonstrate the rapid synthesis of two ribosomal precursor RNA species. One precursor RNA migrating slightly slower than the 1.1×106mol.wt. rRNA appears much less stable than the other precursor RNA, which shows the electrophoretic behaviour of the 0.7×106mol.wt. rRNA. Our observations support the close relationship between bacteria and blue–green algae also with respect to rRNA maturation. The conversion of the ribosomal precursor RNA species into 0.56×106- and 1.1×106-mol.wt. rRNA species requires Mg2+in the incubation medium.

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