Effects of CZB versus medium 199 and of conditioning culture media with either bovine oviductal epithelial cells or buffalo rat liver cells on the development of bovine zygotes derived by in vitro maturation-in vitro fertilization procedures

1993 ◽  
Vol 39 (6) ◽  
pp. 1267-1277 ◽  
Author(s):  
J.J. Hernandez-Ledezma ◽  
C. Villanueva ◽  
J.D. Sikes ◽  
R.M. Roberts
Keyword(s):  
In Vitro Fertilization ◽  
Epithelial Cells ◽  
Rat Liver ◽  
In Vitro Maturation ◽  
Culture Media ◽  
Liver Cells ◽  
Vitro Fertilization ◽  
Maturation In Vitro ◽  
Rat Liver Cells

Coculture of human embryos with buffalo rat liver cells for women with decreased prognosis in in vitro fertilization

1997 ◽  
Vol 177 (2) ◽  
pp. 358-363 ◽  
Author(s):  
Yunxia Hu ◽  
Wayne S. Maxson ◽  
David I. Hoffman ◽  
Susan Eager ◽  
Julie Dupre
Keyword(s):  
In Vitro Fertilization ◽  
Rat Liver ◽  
Liver Cells ◽  
Human Embryos ◽  
Vitro Fertilization ◽  
Rat Liver Cells

scholarly journals The Place of In Vitro Maturation in PCO/PCOS

2018 ◽  
Vol 2018 ◽  
pp. 1-8 ◽  
Author(s):  
Shital Julania ◽  
Melanie L. Walls ◽  
Roger Hart
Keyword(s):  
In Vitro Fertilization ◽  
In Vitro Maturation ◽  
Culture Media ◽  
Polycystic Ovary ◽  
Pregnancy Rates ◽  
Ovary Syndrome ◽  
Successful Pregnancy ◽  
Vitro Fertilization ◽  
In Pregnancy

In vitro maturation (IVM) of human oocytes is an emerging treatment option for women with polycystic ovary/polycystic ovary syndrome (PCO/PCOS) in addition to the standard in vitro fertilization (IVF) treatment. There has been significant improvements in pregnancy rates with IVM over the last two decades. This article reviews the place of IVM for women with PCO/PCOS, placing an emphasis on the predictors of successful pregnancy, optimization of culture media, IVM protocols, pregnancy rates, and neonatal outcomes following IVM treatment.

scholarly journals Expression of the Antioxidant Enzyme and Apoptosis Genes in In vitro Maturation/In vitro Fertilization Porcine Embryos

2004 ◽  
Vol 17 (1) ◽  
pp. 33-38
Author(s):  
H. Y. Jang ◽  
H. S. Kong ◽  
S. S. Lee ◽  
K. D. Choi ◽  
G. J. Jeon ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Antioxidant Enzyme ◽  
In Vitro Maturation ◽  
Vitro Fertilization ◽  
Maturation In Vitro

ТРАНСВАГИНАЛЬНАЯ АСПИРАЦИЯ ФОЛЛИКУЛОВ И СПОСОБНОСТЬ OPU-ООЦИТОВ КОРОВ К ЭМБРИОНАЛЬНОМУ РАЗВИТИЮ В УСЛОВИЯХ IN VITRO

2019 ◽  
pp. 17-19
Author(s):  
G.N. SINGINA ◽  
V. HAVLICEK ◽  
N.P. TARADAYNIK ◽  
R.Y. CHINAROV ◽  
T.E. TARADAYNIK ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Embryo Development ◽  
In Vitro Maturation ◽  
Blastocyst Stage ◽  
Vitro Fertilization ◽  
Maturation In Vitro ◽  
Transvaginal Aspiration ◽  
Cattle Reproduction ◽  
Ovarian Follicular Growth

Представлены результаты трансвагинальной аспирации ооцитов коров, а также оценен их потенциал к эмбриональному развитию после оплодотворения в условиях in vitro. Донорами яйцеклеток являлись половозрелые телки симментальской породы в возрасте 1619 мес. Животныедоноры (n7) перед проведением процедуры Ovum Pickup (OPU) были гормонально обработаны с целью стимуляции роста фолликулов. Количество выделенных ооцитов от индивидуальных доноров составило в среднем 7,7 ооциткумулюсных комплексов (ОКК), что соответствовало степени извлечения 54,57,7. Доля ОКК хорошего качества, рассчитанная от общего числа извлеченных ОКК, между отдельными животными существенно не различалась (значения варьировали от 60,0 до 75,0) и в среднем составила 67,21,9. ОКК с признаками нормальной морфологии подвергали in vitro процедурам созревания, оплодотворения и последующего культивирования до стадии бластоцисты. Доля раздробившихся ооцитов и выход бластоцист после in vitro осеменения яйцеклеток коров равнялась 75,7 и 24,3, соответственно. В целом от одного донора за сессию OPU было получено 1,3 эмбриона на стадии бластоцисты, содержащих в среднем 89,8 ядра. Оцененный способ экстракорпорального оплодотворения OPUооцитов коров позволяет получать эмбрионы, пригодные для замораживания и трансплантации реципиентам и может быть использован в программах по воспроизводству желаемых генотипов у крупного рогатого скота.In the present work, we report the data on transvaginal aspiration of bovine ovarian follicles and estimation of in vitro embryo development competence of collected oocytes. The oocytes were collected by ovum pickup OPU from seven 1619 monthold Simmental heifers, previously hormonallytreated in order to stimulate ovarian follicular growth. In average, 7.7 oocytecumulus complexes (OCCs) per heifer per OPU session were collected that corresponded to 54.57.7 of recovery rate. Morphologically, 60.075.0 of OCCs were the good quality and this rate did not significantly differ between the animals. Good quality OCCs (total n37) were then subjected to in vitro maturation, in vitro fertilization and in vitro embryo development up to blastocyst stage. Cleavage and blastocyst rates were 75.7 и 24.3 , respectively. In total, 1.3 blastocysts were obtained per cow per OPU session in average these blastocysts contained 89.9 cells. In conclusion, we developed the methodology of in vitro fertilization of bovine OPUcollected oocytes that allowed obtaining the blastocysts potentially suitable for freezing and transplantation to recipients. This approach can be used to multiply desired genotypes in cattle reproduction.

A simple method for in vitro maturation, in vitro fertilization, and co-culture of bovine oocytes

1992 ◽  
Vol 14 (3) ◽  
pp. 107-112 ◽  
Author(s):  
L. Zhang ◽  
R. S. Denniston ◽  
R. A. Godke
Keyword(s):  
In Vitro Fertilization ◽  
In Vitro Maturation ◽  
Simple Method ◽  
Vitro Fertilization ◽  
Maturation In Vitro ◽  
Bovine Oocytes

Obstetric Outcomes and Congenital Abnormalities After In Vitro Maturation, In Vitro Fertilization, and Intracytoplasmic Sperm Injection

2007 ◽  
Vol 110 (4) ◽  
pp. 885-891 ◽  
Author(s):  
William M. Buckett ◽  
Ri-Cheng Chian ◽  
Hananel Holzer ◽  
Nicola Dean ◽  
Robert Usher ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Intracytoplasmic Sperm Injection ◽  
In Vitro Maturation ◽  
Congenital Abnormalities ◽  
Obstetric Outcomes ◽  
Vitro Fertilization ◽  
Maturation In Vitro

336 SHEEP OOCYTES MATURED IN A SIMPLEX MEDIUM (HECM-1), AN ANSWER TO IN VITRO FERTILIZATION

2006 ◽  
Vol 18 (2) ◽  
pp. 275
Author(s):  
C. Navarro-Maldonado ◽  
Y. Ducolomb-Ramirez ◽  
A. Galindo-Rodriguez ◽  
A. Rosado-Garcia
Keyword(s):  
In Vitro Fertilization ◽  
Embryo Development ◽  
Oocyte Maturation ◽  
In Vitro Maturation ◽  
Culture Media ◽  
Sperm Quality ◽  
Complete Control ◽  
Vitro Fertilization ◽  
Significant Difference

In vitro maturation and in vitro fertilization (IVM and IVF) of mammalian oocytes still show unsatisfactory results when applied to the study of embryo development. This is probably due to inadequate information about the use of media components and supplements for oocyte maturation and to a discrepancy between results obtained by focusing strictly on oocyte maturation and those that are focused on IVF. A conventional medium that provides adequate results in studies of oocyte maturation (TCM-199) contains hypoxanthine, phosphate ions, and glucose, all known to inhibit embryo development in vitro in some species. In contrast, it has been shown that a simpler medium (HECM-9) increases embryo development in bovine although its use for oocyte maturation has not been defined. This medium contains taurine (an amino acid that reduces intracellular peroxide content) and is supplemented with polyvinyl alcohol (PVA) instead of using protein components, making it a simple defined medium that reduces variability in embryo development. Adding sodium panthothenate to media also confers cell protection against reactive oxygen species. Finally, supplements such as epidermal growth factor (EGF) increase the number of oocytes that complete maturation (Metaphase II, MII) and facilitate embryo development. An adequate combination of our knowledge about in vitro maturation and fertilization of oocytes, together with the requirements for embryo development, is important for the preparation of culture media to study regulatory mechanisms for embryo development and to increase the number of viable and normal term individuals. In this study we compared the effects of HECM-9 (containing panthothenate) vs. TCM-199 (both media supplemented with PVA, EGF, and FSH/LH) on the integrated processes involving IVM and IVF. No significant differences were found between the results obtained with these media in relation to oocyte maturation (65% MII for HECM-9 vs. 71% for TCM-199); however, those oocytes matured in HECM-9 showed a highly significant difference in in vitro fertilization using a conventional IVF medium (SOFm) (25% in HECM-9 vs. 6% in TCM-199). Maturation results were relatively low but in accordance with those reported by other groups, whereas IVF results are lower than those reported in the literature, perhaps because we have been using frozen and thawed samples and do not have complete control over the sperm quality. At present, we are extending our investigation using fresh semen samples.

Sign in / Sign up

Export Citation Format

Share Document