VIL-A1, A monoclonal antibody reactive with common acute lymphatic leukemia cells

Abstract B- and T-cell markers were studied in a patient with chronic lymphocytic leukemia and erythroderma. The absence of immunoglobulin, complement receptor, and Fc receptor, and the presence of sheep erythrocyte receptor and T-cell antigen on the membrane of the leukemic cells classified them as thymus derived. Using quantitative microphotometric immunoautoradiography, surface antigen densities were measured at the cellular level with the following results: (1) The density of T-antigenic sites was less on leukemic cells compared to normal T lymphocytes. (2) The T-antigen densities of leukemic lymphocytes varied less from cell to cell forming a homogeneous peak in histograms. (3) An Ig density of normal B lymphocytes was demonstrated on the residual T-antigen-negative cells. The results were qualitatively confirmed by direct immunofluorescence and electron microscopy with peroxidase-labeled antibodies. Furthermore, the surface antigens were quantitative microcomplement fixation test which revealed reduced binding of anti-T-cell antibodies and complement, and no antiglobulin fixation on the leukemic lymphocytes. Since lymphocytes with normal T-antigen concentration could not be found among the leukemic T lymphocytes, a lack of normal T cells was assumed. The findings that there was a decrease and altered distribution of surface markers on chronic lymphatic leukemia cells of the B- and T-cell type are discussed as further arguments referring to their clonal origin.

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Comparative study of alkaline phosphatase activity in lymphocytes, mitogen-induced blasts, lymphoblastoid cell lines, acute myeloid leukemia, and chronic lymphatic leukemia cells

Proceedings of the National Academy of Sciences ◽

10.1073/pnas.73.5.1432 ◽

1976 ◽

Vol 73 (5) ◽

pp. 1432-1436 ◽

Cited By ~ 31

Author(s):

H Neumann ◽

E Klein ◽

R Hauck-Granoth ◽

S Yachnin ◽

H Ben-Bassat

Keyword(s):

Alkaline Phosphatase ◽

Acute Myeloid Leukemia ◽

Alkaline Phosphatase Activity ◽

Myeloid Leukemia ◽

Leukemia Cells ◽

Lymphoblastoid Cell Lines ◽

Chronic Lymphatic Leukemia ◽

Lymphatic Leukemia ◽

Lymphatic Leukemia Cells ◽

Acute Myeloid

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Combine use of Rituximab and Campath-1H increase complement-dependent killing of non-Hodgkin's lymphoma and chronic lymphatic leukemia cells

Molecular Immunology ◽

10.1016/j.molimm.2007.06.093 ◽

2007 ◽

Vol 44 (16) ◽

pp. 3948

Author(s):

P. Macor ◽

M. Caizzi ◽

E. Secco ◽

V. Gattei ◽

F. Tedesco

Keyword(s):

Hodgkin’S Lymphoma ◽

Hodgkin's Lymphoma ◽

Leukemia Cells ◽

Chronic Lymphatic Leukemia ◽

Lymphatic Leukemia ◽

Non Hodgkin’S Lymphoma ◽

Non Hodgkin's Lymphoma ◽

Lymphatic Leukemia Cells

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The Surface Phenotype of Lymphatic Leukemia, Cells: An Immunoscanning Electron Microscopy Study

Leukemia & Lymphoma ◽

10.3109/10428198909042455 ◽

1989 ◽

Vol 1 (1) ◽

pp. 29-34 ◽

Cited By ~ 3

Author(s):

Davide Soligo ◽

Giancarlo Balercia ◽

Francesco Osculati ◽

Nadia Quirici ◽

Andrea Sbarbati ◽

...

Keyword(s):

Electron Microscopy ◽

Microscopy Study ◽

Electron Microscopy Study ◽

Leukemia Cells ◽

Surface Phenotype ◽

Lymphatic Leukemia ◽

Lymphatic Leukemia Cells

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Decrease and altered distribution of human T antigen on chronic lymphatic leukemia cells of T type, suggesting a clonal origin

Blood ◽

10.1182/blood.v47.5.723.bloodjournal475723 ◽

1976 ◽

Vol 47 (5) ◽

pp. 723-736

Author(s):

E Thiel ◽

H Rodt ◽

D Huhn ◽

S Thierfelder

Keyword(s):

T Cell ◽

T Lymphocytes ◽

Sheep Erythrocyte ◽

T Antigen ◽

Leukemic Cells ◽

Leukemia Cells ◽

Chronic Lymphatic Leukemia ◽

Lymphatic Leukemia ◽

Clonal Origin ◽

Lymphatic Leukemia Cells

B- and T-cell markers were studied in a patient with chronic lymphocytic leukemia and erythroderma. The absence of immunoglobulin, complement receptor, and Fc receptor, and the presence of sheep erythrocyte receptor and T-cell antigen on the membrane of the leukemic cells classified them as thymus derived. Using quantitative microphotometric immunoautoradiography, surface antigen densities were measured at the cellular level with the following results: (1) The density of T-antigenic sites was less on leukemic cells compared to normal T lymphocytes. (2) The T-antigen densities of leukemic lymphocytes varied less from cell to cell forming a homogeneous peak in histograms. (3) An Ig density of normal B lymphocytes was demonstrated on the residual T-antigen-negative cells. The results were qualitatively confirmed by direct immunofluorescence and electron microscopy with peroxidase-labeled antibodies. Furthermore, the surface antigens were quantitative microcomplement fixation test which revealed reduced binding of anti-T-cell antibodies and complement, and no antiglobulin fixation on the leukemic lymphocytes. Since lymphocytes with normal T-antigen concentration could not be found among the leukemic T lymphocytes, a lack of normal T cells was assumed. The findings that there was a decrease and altered distribution of surface markers on chronic lymphatic leukemia cells of the B- and T-cell type are discussed as further arguments referring to their clonal origin.

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Cytotoxic Polyketide Metabolites from a Marine Mesophotic Zone Chalinidae Sponge-Associated Fungus Pleosporales sp. NBUF144

Marine Drugs ◽

10.3390/md19040186 ◽

2021 ◽

Vol 19 (4) ◽

pp. 186

Author(s):

Jing Zhou ◽

Hairong Zhang ◽

Jing Ye ◽

Xingxin Wu ◽

Weiyi Wang ◽

...

Keyword(s):

Mass Spectra ◽

Spectroscopic Analysis ◽

2D Nmr ◽

Leukemia Cells ◽

Ionization Mass ◽

X Ray Diffraction ◽

X Ray ◽

Lymphatic Leukemia ◽

Ic50 Value ◽

Lymphatic Leukemia Cells

Two new polyketide natural products, globosuxanthone F (1), and 2′-hydroxy bisdechlorogeodin (2), were isolated from the fungus Pleosporales sp. NBUF144, which was derived from a 62 m deep Chalinidae family sponge together with four known metabolites, 3,4-dihydroglobosuxanthone A (3), 8-hydroxy-3-methylxanthone-1-carboxylate (4), crosphaeropsone C (5), and 4-megastigmen-3,9-dione (6). The structures of these compounds were elucidated on the basis of extensive spectroscopic analysis, including 1D and 2D NMR and high-resolution electrospray ionization mass spectra (HRESIMS) data. The absolute configuration of 1 was further established by single-crystal X-ray diffraction studies. Compounds 1-5 were evaluated for cytotoxicity towards CCRF-CEM human acute lymphatic leukemia cells, and it was found that 1 had an IC50 value of 0.46 µM.

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