scholarly journals Decrease and altered distribution of human T antigen on chronic lymphatic leukemia cells of T type, suggesting a clonal origin

Blood ◽  
1976 ◽  
Vol 47 (5) ◽  
pp. 723-736
Author(s):  
E Thiel ◽  
H Rodt ◽  
D Huhn ◽  
S Thierfelder
Keyword(s):  
T Cell ◽  
T Lymphocytes ◽  
Sheep Erythrocyte ◽  
T Antigen ◽  
Leukemic Cells ◽  
Leukemia Cells ◽  
Chronic Lymphatic Leukemia ◽  
Lymphatic Leukemia ◽  
Clonal Origin ◽  
Lymphatic Leukemia Cells

B- and T-cell markers were studied in a patient with chronic lymphocytic leukemia and erythroderma. The absence of immunoglobulin, complement receptor, and Fc receptor, and the presence of sheep erythrocyte receptor and T-cell antigen on the membrane of the leukemic cells classified them as thymus derived. Using quantitative microphotometric immunoautoradiography, surface antigen densities were measured at the cellular level with the following results: (1) The density of T-antigenic sites was less on leukemic cells compared to normal T lymphocytes. (2) The T-antigen densities of leukemic lymphocytes varied less from cell to cell forming a homogeneous peak in histograms. (3) An Ig density of normal B lymphocytes was demonstrated on the residual T-antigen-negative cells. The results were qualitatively confirmed by direct immunofluorescence and electron microscopy with peroxidase-labeled antibodies. Furthermore, the surface antigens were quantitative microcomplement fixation test which revealed reduced binding of anti-T-cell antibodies and complement, and no antiglobulin fixation on the leukemic lymphocytes. Since lymphocytes with normal T-antigen concentration could not be found among the leukemic T lymphocytes, a lack of normal T cells was assumed. The findings that there was a decrease and altered distribution of surface markers on chronic lymphatic leukemia cells of the B- and T-cell type are discussed as further arguments referring to their clonal origin.

scholarly journals Decrease and altered distribution of human T antigen on chronic lymphatic leukemia cells of T type, suggesting a clonal origin

Blood ◽  
1976 ◽  
Vol 47 (5) ◽  
pp. 723-736 ◽  
Author(s):  
E Thiel ◽  
H Rodt ◽  
D Huhn ◽  
S Thierfelder
Keyword(s):  
T Cell ◽  
T Lymphocytes ◽  
Sheep Erythrocyte ◽  
T Antigen ◽  
Leukemic Cells ◽  
Leukemia Cells ◽  
Chronic Lymphatic Leukemia ◽  
Lymphatic Leukemia ◽  
Clonal Origin ◽  
Lymphatic Leukemia Cells

Abstract B- and T-cell markers were studied in a patient with chronic lymphocytic leukemia and erythroderma. The absence of immunoglobulin, complement receptor, and Fc receptor, and the presence of sheep erythrocyte receptor and T-cell antigen on the membrane of the leukemic cells classified them as thymus derived. Using quantitative microphotometric immunoautoradiography, surface antigen densities were measured at the cellular level with the following results: (1) The density of T-antigenic sites was less on leukemic cells compared to normal T lymphocytes. (2) The T-antigen densities of leukemic lymphocytes varied less from cell to cell forming a homogeneous peak in histograms. (3) An Ig density of normal B lymphocytes was demonstrated on the residual T-antigen-negative cells. The results were qualitatively confirmed by direct immunofluorescence and electron microscopy with peroxidase-labeled antibodies. Furthermore, the surface antigens were quantitative microcomplement fixation test which revealed reduced binding of anti-T-cell antibodies and complement, and no antiglobulin fixation on the leukemic lymphocytes. Since lymphocytes with normal T-antigen concentration could not be found among the leukemic T lymphocytes, a lack of normal T cells was assumed. The findings that there was a decrease and altered distribution of surface markers on chronic lymphatic leukemia cells of the B- and T-cell type are discussed as further arguments referring to their clonal origin.

Appearance of the T-cell marker CD8 on B chronic lymphatic leukemia cells in long-term cultures

1991 ◽  
Vol 34 (3) ◽  
pp. 181-185 ◽  
Author(s):  
Ruth Farkas ◽  
Shlomo Ben-Efraim ◽  
Yosef Manor ◽  
Israel Zan-Bar ◽  
Abraham Klajman
Keyword(s):  
T Cell ◽  
Leukemia Cells ◽  
Chronic Lymphatic Leukemia ◽  
Cell Marker ◽  
Lymphatic Leukemia ◽  
Lymphatic Leukemia Cells

scholarly journals Identification of a p69,71 complex expressed on human T cells sharing determinants with B-type chronic lymphatic leukemic cells.

1980 ◽  
Vol 151 (6) ◽  
pp. 1539-1544 ◽  
Author(s):  
C Y Wang ◽  
R A Good ◽  
P Ammirati ◽  
G Dymbort ◽  
R L Evans
Keyword(s):  
T Cells ◽  
B Cells ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sheep Erythrocyte ◽  
Sodium Dodecyl ◽  
Peripheral Blood Lymphocytes ◽  
Leukemic Cells ◽  
Target Antigen ◽  
Chronic Lymphatic Leukemia ◽  
Lymphatic Leukemia

In the course of generating monoclonal antibodies to human thymus-dependent differentiation antigens, we were able to define specificities shared by T cells and by cells from patients with chronic lymphatic leukemia that were not detectable on normal B cells. In particular, one of these antibodies was reactive by indirect immunofluorescence with greater than 95% of the thymocytes and 80--95% of nonadherent sheep erythrocyte-rosetting peripheral blood lymphocytes (PBL), but was unreactive with normal B cells or cell lines derived from PBL by Epstein-Barr virus transformation. However, the leukemic cells from 11 of 14 patients with B-type chronic lymphatic leukemia were found to express detectable concentrations of this surface determinant. The target antigen recognized by this monoclonal antibody was shown by immunoprecipitation and sodium dodecyl sulfate-polyacrylamide gel electrophoresis to be a p69,71 complex. Our findings suggest a possible relationship between this antigen and the previously described GIX system in the mouse.

scholarly journals Differential Effects of Tea Extracts on Growth and Cytokine Production by Normal and Leukemic Human Leukocytes

2012 ◽  
Vol 2 (4) ◽  
pp. 72 ◽  
Author(s):  
Diana Bayer ◽  
Jonathon Jansen ◽  
Lisa A. Beltz
Keyword(s):  
T Lymphocytes ◽  
T Lymphocyte ◽  
Mononuclear Cells ◽  
Cytokine Production ◽  
Lymphoblastic Leukemia ◽  
Interleukin 2 ◽  
Leukemic Cells ◽  
Leukemia Cells ◽  
Peripheral Blood Mononuclear ◽  
Normal Cells

Background: Tea is one of the world’s most highly consumed beverages, second only to water. It is affordable and abundant and thus has great potential for improving health of those in both developed and developing areas. Green, oolong, and black teas differ in the extent of fermentation and types of bioactive polyphenols produced. Green tea and its major polyphenol decrease growth of some cancer cells and effect production of immune system cytokines. This study compares the effects of different types of tea extracts on viability and cytokine production by normal and leukemic human T lymphocytes. Generation of the toxic reactive oxygen species H2O2 by extracts was also examined.Methods: The Jurkat T lymphoblastic leukemia cells and mitogen-stimulated normal human peripheral blood mononuclear cells were used in this study. Cell viability was determined by (3-4,5-dimethylthiamizol-2-yl)-diphenyltetrazolium bromide) assay and production of interleukin-2 by Enzyme-Linked ImmunoSorbent Assay. Levels of H2O2 generated by tea extracts were determined using the xylenol-orange method.Results: We found that green, oolong, and black tea extracts differentially effect the growth and viability of T lymphoblastic leukemia cells and normal peripheral blood mononuclear cells, substantially decreasing both growth and viability of leukemic T lymphocytes and having much lesser effects on their normal counterparts. Tea extracts also had differential effects on the production of the T lymphocyte growth factor interleukin-2, significantly decreasing production by leukemic cells while having only minor effects on normal cells. All three extracts induced H2O2 generation, with green and oolong tea extracts having the greatest effect. Leukemic cells were much more susceptible to growth inhibition and killing by H2O2 than normal lymphocytes. Conclusions: The three tea extracts studied altered leukemic T lymphocyte functions, decreasing cell viability, growth, and production of a major cell growth factor and the H2O2 generated by solutions of extracts may be partially responsible. Normal cells were affected to a far lesser degree by tea extracts and are also more resistant to killing by H2O2 than leukemic cells. This study has implications for using tea extracts for chemotherapeutic and immunomodulatory purposes.Key Words: Tea extracts, interleukin-2, hydrogen peroxide, leukemia, T lymphocytes

scholarly journals PARA-AMINOBENZOIC ACID IN LEUKEMIA

Blood ◽  
1948 ◽  
Vol 3 (7) ◽  
pp. 780-792 ◽  
Author(s):  
C. J. D. ZARAFONETIS ◽  
G. A. ANDREWS ◽  
M. C. MEYERS ◽  
F. H. BETHELL
Keyword(s):  
Inhibitory Action ◽  
White Cell Count ◽  
Myelogenous Leukemia ◽  
Leukemic Cells ◽  
Spleen Size ◽  
Chronic Lymphatic Leukemia ◽  
Aminobenzoic Acid ◽  
Lymphatic Leukemia ◽  
Yield Information ◽  
Leukocyte Counts

Abstract Para-aminobenzoic acid, administered in large doses as sodium para-aminobenzoate caused a striking lowering of the leukocyte counts in five patients with chronic myelogenous leukemia and in one patient with subacute myelogenous leukemia. NaPAB caused less definite decreases of the white cell count in two patients with chronic lymphatic leukemia, but the periods of administration may have been too short to obtain maximal effects. In every instance, there was a prompt rise in the number of leukocytes when the administration of NaPAB was stopped. Although there was decrease in spleen size in some of the patients, the objective clinical improvement was but slight and temporary. All patients receiving NaPAB in large doses had concomitant glycosuria, apparently on a renal basis. It is to be emphasized that NaPAB is not considered a practical adjunct to the therapy of leukemia at this time. Rather, it is hoped that studies of the cellular chemistry involved in the apparent inhibitory action of NaPAB may yield information concerning the disordered metabolism of leukemic cells.

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