The chicken macrophage response to carbohydrate-based irritants: Temporal changes in peritoneal cell populations

1988 ◽  
Vol 12 (1) ◽  
pp. 109-119 ◽  
Author(s):  
Y. Chu ◽  
R.R. Dietert
2019 ◽  
Vol 50 (1) ◽  
Author(s):  
Min Feng ◽  
Tingting Xie ◽  
Yuanfang Li ◽  
Nan Zhang ◽  
Qiuyuan Lu ◽  
...  

2019 ◽  
Vol 41 (9) ◽  
Author(s):  
Roberta Reis Soares ◽  
Clarissa F. Cunha ◽  
Raquel Ferraz‐Nogueira ◽  
Alessandro Marins‐dos‐Santos ◽  
Rodrigo Nunes Rodrigues‐da‐Silva ◽  
...  

2020 ◽  
Vol 51 (1) ◽  
Author(s):  
Lianci Peng ◽  
Maaike R. Scheenstra ◽  
Roel M. van Harten ◽  
Henk P. Haagsman ◽  
Edwin J. A. Veldhuizen

Abstract Cathelicidins (CATHs) play an important role in the innate immune response against microbial infections. Among the four chicken cathelicidins, CATH-B1 is studied the least. In this study, the effect of CATH-B1 on the macrophage response towards avian pathogenic E. coli (APEC) and bacterial ligands was investigated. Our results show that APEC induced CATH-B1 gene expression in both a chicken macrophage cell line (HD11 cells) and primary macrophages, while expression of the other three CATHs was virtually unaffected. While the antimicrobial activity of CATH-B1 is very low under cell culture conditions, it enhanced bacterial phagocytosis by macrophages. Interestingly, CATH-B1 downregulated APEC-induced gene expression of pro-inflammatory cytokines (IFN-β, IL-1β, IL-6 and IL-8) in primary macrophages. In addition, CATH-B1 pre-incubated macrophages showed a significantly higher gene expression of IL-10 after APEC challenge, indicating an overall anti-inflammatory profile for CATH-B1. Using isothermal titration calorimetry (ITC), CATH-B1 was shown to bind LPS. This suggests that CATH-B1 reduces toll like receptor (TLR) 4 dependent activation by APEC which may partly explain the decreased production of pro-inflammatory cytokines by macrophages. On the contrary, direct binding of CATH-B1 to ODN-2006 enhanced the TLR21 dependent activation of macrophages as measured by nitric oxide production. In conclusion, our results show for the first time that CATH-B1 has several immunomodulatory activities and thereby could be an important factor in the chicken immune response.


1987 ◽  
Vol 21 (4) ◽  
pp. 419-428 ◽  
Author(s):  
A. Pizzoferrato ◽  
A. Vespucci ◽  
G. Ciapetti ◽  
S. Stea ◽  
C. Tarabusi

1989 ◽  
Vol 118 (1) ◽  
pp. 178-191 ◽  
Author(s):  
Meryle J. Melnicoff ◽  
Paul K. Horan ◽  
Page S. Morahan

1978 ◽  
Vol 16 (6) ◽  
pp. 901-910 ◽  
Author(s):  
D.A. Stringfellow ◽  
F.A. Fitzpatrick ◽  
F.F. Sun ◽  
J.C. McGuire

Biomaterials ◽  
2018 ◽  
Vol 182 ◽  
pp. 202-215 ◽  
Author(s):  
Kelly M. Hotchkiss ◽  
Nicholas M. Clark ◽  
Rene Olivares-Navarrete

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